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Parlindo, Fitra
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Medicine & Pharmacology

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Penurunan Risiko Perkembangan Cendawan Terbawa Benih Hotong asal Pulau Buru melalui Perlakuan Air Panas PARLINDO, FITRA; Khairani, Hagia Sophia; Ardie, Sintho Wahyuning
Jurnal Fitopatologi Indonesia Vol. 18 No. 6 (2022): November 2022
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.18.6.264-268

Abstract

Penurunan Risiko Perkembangan Cendawan Terbawa Benih Hotong (Setaria italica) Asal Pulau Buru Melalui Perlakuan Air Panas Penggunaan benih sehat yang bebas cendawan terbawa benih merupakan faktor penting untuk meningkatkan produksi hotong (Setaria italica). Penelitian ini bertujuan untuk memperkaya teknik eliminasi cendawan terbawa benih melalui perlakuan air panas terhadap benih hotong asal Pulau Buru. Identifikasi cendawan terbawa benih menunjukkan bahwa Boeremia merupakan cendawan dominan pada benih hotong asal Pulau Buru. Perlakuan air panas dengan kombinasi suhu (50 ℃, 52 ℃, 54 ℃) dan lama perlakuan (15, 20, 25 menit) diujikan untuk mencari perlakuan yang paling efektif menekan pertumbuhan cendawan tetapi tidak mengganggu perkecambahan benih. Penelitian ini menunjukkan bahwa perlakuan air panas pada suhu 50 ℃ selama 25 menit mampu menurunkan infestasi cendawan pada perlakuan in vitro dan ad planta berturut-turut sebesar 57% dan 55%. Hal ini mengindikasikan bahwa metode ini dapat diandalkan untuk menyelamatkan kerugian produksi benih hotong akibat perkembangan cendawan patogen.
Direct Isolation Method for Tomato-Infecting Phytophthora infestans : Metode Isolasi Langsung untuk Phytophthora infestans yang Menginfeksi Tanaman Tomat Parlindo, Fitra; Khairani, Hagia Sophia; Papona, Alisya Talita; Tondok, Efi Toding
Jurnal Fitopatologi Indonesia Vol. 20 No. 2 (2024): Maret 2024
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.20.2.101-107

Abstract

Infection of Phytophthora infestans at high altitudes in tropical regions causes symptoms of tomato late blight throughout the year. Even though it is very easy to find in the field, P. infestans is often very difficult to isolate aseptically in the laboratory. This study aims to evaluate direct isolation techniques that can increase the success of isolating P. infestans. Isolation was carried out on a non-specific medium, consisting of potato dextrose agar (PDA), corn meal agar (CMA), oatmeal agar (OMA), and water agar (WA) with three alternative types of antibiotics, i.e. chloramphenicol, amoxicillin, and rifampicin. Observations were carried out to determine the effect of the medium on sporangia induction and the age of the original colony’s growth in CMA and OMA media. The results showed that the isolation of P. infestans using the direct method was successfully carried out on non-specific PDA, CMA, OMA and WA medium. The highest isolation success rate was obtained on CMA medium with the addition of 50 mg L-1 rifampicin. The fastest sporangia induction (8 days) was shown by colonies grown on OMA medium with the addition of 50 mg L-1 rifampicin based on the category of abundant sporangia after 20 days of incubation. Rejuvenation of P. infestans colonies for research purposes in the laboratory is recommended to be carried out routinely twice a month. This research provides practical guidance for understanding the bioecology of P. infestans infecting tomato plants, especially for further study on oomycetes fungi.
Co-Authors Efi Toding Tondok Hagia Sophia Khairani Papona, Alisya Talita Sintho Wahyuning Ardie