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All Journal Jurnal Ilmu Kimia Universitas Brawijaya
Roosdiana, Anna
Brawijaya University
Chemical Engineering, Chemistry & Bioengineering Chemistry

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OPTIMASI AMOBILISASI PEKTINASE DARI Bacillus subtilis MENGGUNAKAN Ca-ALGINAT-KITOSAN Siswanti, Chandra Ayu; Roosdiana, Anna; Sutrisno, Sutrisno
Jurnal Ilmu Kimia Universitas Brawijaya Vol 1, No 2 (2013)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Abstract

Pektinase bebas tidak dapat digunakan lebih dari satu kali pemakaian, sehingga perlu dilakukan teknik amobilisasi agar dapat digunakan secara berulang. Penelitian ini mempelajari tentang kondisi optimum proses amobilisasi pektinase pada matriks Ca-alginat-kitosan. Pektinase diisolasi dari Bacillus subtilis, dimurnikan dengan metode pemurnian bertingkat menggunakan amonium sulfat fraksi 20–60% dan dilanjutkan dengan dialisis. Mikroenkapsulasi Ca-alginat-kitosan dibuat dengan mencampurkan pektinase pada variasi 0,943; 1,886; 2,83; 3,774; 4,717 mg/mL dengan Na-alginat pada variasi 1,5; 2; 2,5; 3; 3,5% (w/v) dan meneteskannya pada larutan CaCl2 0,15 M. Manik Ca-alginat selanjutnya disuspensikan pada kitosan 1,5% dan diteteskan pada larutan Na5P3O10 3%, sehingga terbentuk hibrid mikrokapsul Ca-alginat-kitosan. Hasil penelitian menunjukkan bahwa pada konsentrasi optimum Na-alginat 3% (w/v) dan konsentrasi pektinase optimum 2,83 mg/mL dapat menjebak pektinase sebanyak 2,83 mg/gram Ca-alginat-kitosan dengan aktivitas sebesar 193,3 unit. Kata kunci: aktivitas, Bacillus subtilis, Ca-alginat-kitosan, pektinase
STUDI PEMBERIAN EKSTRAK RUMPUT LAUT COKLAT (Sargassum prismaticum) TERHADAP KADAR MDA DAN HISTOLOGI JARINGAN PANKREAS PADA TIKUS Rattus norvegicus DIABETES MELITUS TIPE 1 HASIL INDUKSI MLD-STZ (MULTIPLE LOW DOSE - STREPTOZOTOCIN) Dewi, Devis Resita; Aulanni'am, Aulanni'am; Roosdiana, Anna
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 1 (2013)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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The aim of this research was to investigate the effect of brown seaweed (Sargassum prismaticum) extract to decreased MDA levels and improvement of pancreatic tissue histology on rats Rattus norvegicus type 1 diabetes mellitus (DM). Type 1 diabetic rats induced by intraperitonial (i.p) injection of Multiple Low Dose-Streptozotocin (MLD-STZ) with 20 mg/kg (bw) dose for 5 consecutive days. MDA was measured by UV-Vis spectrophotometry method at a wavelength of 540 nm using TBA (Thiobarbituric acid) reagent. Histology of pancreatic tissue performed using Hematoxylen-Eosin staining method. In this research, rats were grouped into 6 treatment groups: the group of control rats, type 1 diabetic rats exposed to MLD-STZ, and type 1 diabetic rats exposed to MLD-STZ than treated by brown seaweed extract orally with 0.8 g/tail dose by 2 mL per day for 1, 3, 5, and 7 days. The results of this research obtained average MDA levels of each group of consecutive treatments were 2.507; 6.770; 5.543; 4.444; 3.734 and 2.980 mg/mL. Therapy with Sargassum prismaticum extract for 7 days can reduce MDA levels of type 1 diabetic rats by 55.98% indicating level of (6.770 ± 0.027) mg/dL to be (2.980 ± 0.017) mg/dL and able to improved pancreatic tissue histology type 1 diabetic rats. Key words: diabetes mellitus, histology, MDA, pancreas, Sargassum prismaticum
OPTIMASI AMOBILISASI UREASE DARI Schizzosaccharomyces pombe MENGGUNAKAN MATRIK KITOSAN-NATRIUM TRIPOLIFOSFAT Fatmawati, Ivone; Prasetyawan, Sasangka; Roosdiana, Anna
Jurnal Ilmu Kimia Universitas Brawijaya Vol 2, No 1 (2013)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Determination of optimum condition of immobilized urease using chitosan-sodium tripolyphosphate has been done. Urease was isolated from Schizzosaccharomyces pombe and was purified through fractional purification method using ammonium sulphate saturation of 30– 45%. Urease is an enzyme that has the ability to hydrolyze of urea into carbon dioxide and ammonia. The activity of urease was defined as the amount of micromol ammonia per mL enzyme per minute and resulted ammonia was analyzed by spectrofotometric method using Nessler reagen. The optimum condition of immobilized urease was observed by determining the highest activity in various chitosan concentration (1.5; 2.0; 2.5; 3.0; 3.5) % w/v and urease concentration (0.840; 1.680; 2.520; 3.360; 4.200) mg/mL. The result of this experiment showed that the optimum chitosan concentration was 2.5% w/v while the optimum urease concentration was 2.520 mg/mL with the highest activity of  0.57 Units. Key words: activity, chitosan-sodium tripolyphosphate, Schizzosaccharomyces pombe, urease.  
SINTESIS KERAMIK BERPORI BERBAHAN DASAR LUMPUR LAPINDO MENGGUNAKAN CETAKAN NATA DE COCO Afida, Laely Nur; Tjahjanto, Rachmat Triandi; Roosdiana, Anna
Jurnal Ilmu Kimia Universitas Brawijaya Vol 1, No 1 (2014)
Publisher : Jurusan Kimia, FMIPA Universitas Brawijaya

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Lumpur Lapindo mengandung Fe2O3, SiO2, dan Al2O3 yang berpotensi untuk dijadikan bahan dasar keramik. Tujuan dari penelitian ini adalah untuk mengetahui karakter fisik keramik berpori yang dibuat menggunakan cetakan nata de coco serta mengetahui pengaruh variasi kadar gula dalam sintesis. Sintesis keramik diawali dengan preparasi sampel dengan proses refluks menggunakan HCl untuk mengurangi kadar Fe2O3 serta penambahan larutan Mg(II) 1% untuk meningkatkan kekerasan keramik. Terdapat dua kelompok sampel, A dan B. Keramik A disiapkan dengan mencampurkan lumpur Lapindo dan larutan gula 10%, keramik B menggunakan larutan gula 12%. Karakterisasi keramik berpori meliputi densitas dan luas permukaan yang menggunakan metilen biru. Hasil penelitian menunjukkan sampel A1 memiliki luas permukaan maksimum (8,57 m2/g ), sedangkan densitas terendah (0,99 g/cm3) pada sampel B4. Perbedaan konsentrasi gula tidak berpengaruh secara signifikan terhadap sifat keramik. Kata kunci: densitas, kadar gula, keramik, luas permukaan, lumpur Lapindo
Co-Authors Afida, Laely Nur Aulanni'am, Aulanni'am Dewi, Devis Resita Fatmawati, Ivone Prasetyawan, Sasangka Rachmat Triandi Tjahjanto Siswanti, Chandra Ayu Sutrisno, Sutrisno