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All Journal Jurnal Pembelajaran dan Biologi Nukleus Makara Journal of Science
Utomo, Doddy Irawan Setyo
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media

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Enhancement of Omega-3 DHA content by Feeding Hermetia illucens and Microalgae Aurantiochytrium sp. on Catfish Yarza, Husnin Nahry; Hutari, Andri; Meitiyani, Meitiyani; Nisaa, Ranti An; Rhidailahi, Putri Ayu; Prameswari, Dhanti Cyntia; Utomo, Doddy Irawan Setyo
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i1.5725

Abstract

The growing demand for sustainable and high-quality animal feed has led to exploring alternative sources of protein and other essential nutrients. The black soldier fly (BSF) maggots Hermetia illucens and microalgae Aurantiochytrium sp. are promising candidates. Both have shown significant potential due to their nutritional profiles and environmental benefits. This study aimed to compare the fatty acid content of two groups of Sangkuriang catfish. One group was given commercial animal feed, while the other group was fed with a combination of Aurantiochytrium sp. microalga biomass feed, Hermetia illucens maggot, rice bran, and corn flour. The catfish fed with Aurantiochytrium sp. microalga biomass, Hermetia illucens maggot, rice bran, and corn flour had a different fatty acid composition than the control group. This alternative diet led to an increase in the omega-3 docosahexaenoic fatty acid (DHA) content in the catfish. The study results suggest that fish feed containing Hermetia illucens maggots and microalgae Aurantiochytrium sp. can enhance fish's beneficial nutrition after being fed this particular feed mixture. 
Cloning and Expression of SCAMP3 in Escherichia coli BL21(DE3) with In Silico Sequence-Based Cancer Epitopes Prediction Rajagukguk, Selly Setiati; Pambudi, Sabar; Dwiranti, Astari; Utomo, Doddy Irawan Setyo; Bowolaksono, Anom
Makara Journal of Science Vol. 29, No. 1
Publisher : UI Scholars Hub

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Abstract

Secretory carrier membrane protein 3 (SCAMP3) is a crucial membrane protein involved in intracellular vesicle traffick-ing and exocytosis. The SCAMP3 expression has been observed in diverse cancer types, such as melanoma, glioma, hepatocellular and breast cancer. Increased SCAMP3 expression has been reported in certain cancer cells relative to that in normal cells, suggesting the potential role of SCAMP3 in cancer development or progression. In this study, we successfully cloned and expressed SCAMP3 in Escherichia coli strain BL21(DE3). SCAMP3 was amplified and insert-ed directionally into the prokaryotic expression vector pET21d(+). The transformation of recombinant plasmid into E. coli BL21(DE3) cells were performed for the protein expression. SDS–PAGE and Western blotting were performed to detect the expression product induced by IPTG, which confirmed the presence of a recombinant pET21d(+)-SCAMP3 at 38-kDa protein weight. Bioinformatics analyses helped discover several possible epitopes distributed throughout the SCAMP3 protein sequence. These findings together serve as a basis for future biochemical and functional studies on this important membrane protein alongside immunotherapy research related to SCAMP3 as a cancer biomarker.
Co-Authors ANOM BOWOLAKSONO Dwiranti, Astari Hutari, Andri Meitiyani, Meitiyani Nisaa, Ranti An Pambudi, Sabar Prameswari, Dhanti Cyntia Rajagukguk, Selly Setiati Rhidailahi, Putri Ayu Yarza, Husnin Nahry