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All Journal HAYATI Journal of Biosciences Microbiology Indonesia Media Penelitian dan Pengembangan Kesehatan Jurnal Sain Peternakan Indonesia Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Current Research on Biosciences and Biotechnology Igya ser hanjop: Jurnal Pembangunan Berkelanjutan Makara Journal of Science Journal of Global Pharma Technology Unnes Journal of Public Health

ANOM BOWOLAKSONO

Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Indonesia, Kampus Depok, Depok 16424

Author-ID : 1142347

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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TELAAH ANTI KLASTOGENIK EKSTRAK KASAR RIMPANG KENCUR (KAEMPFERIA galanga L.) TERHADAP AKTIVITAS KLASTOGENIK MITOMISIN C PADA ERITROSIT POLIKROMATIK MENCIT Setiorini, Setiorini; Bowolaksono, Anom
Media Penelitian dan Pengembangan Kesehatan Vol 11, No 3 Sept (2001)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mpk.v11i3 Sept.684.

Penelitian ini dilakukan untuk mengetahui potensi antiklastogenik rimpang kencur terhadap aktivitas klastogenik mitomicin C eritrosit polikromatik mencit. Penelitian ini menggunakan uji mikronukleus 30 ekor mencit (Mus musculus L.) jantan galur Swiss yang dibagi menjadi 6 kelompok perlakuan, masing-masing 5 ulangan. Perlakuan I adalah kontrol, yaitu mencit yang diberi dengan akuades murni. Perlakuan II, III, IV, V, dan VI yaitu mencit yang diberi ekstrak kencur dengan dosis berturut-turut 6,25; 12,5; 25; 50; dan 100 mg/kg berat badan (bb). Mulut mencit diberi ini selama 7 hari berturut-turut. Pada hari ke-8 mencit-mencit tersebut disuntik dengan mitomicin C (MMC) secara intra peritoneal sebanyak 1,5 mg/kg bb dan dibiarkan selama 30 jam. Setelah itu dibuat sediaan sumsum tulang. Hasil penelitian ini menyimpulkan bahwa ekstrak rimpang kencur dengan dosis 6,25; 12,5; 25; dan 50 mg/kg BB dapat menghambat kerusakan sitogenetik yang diinduksi oleh MMC pada eritrosit polikromatik sumsum tulang mencit. Sedangkan, dosis 100 mg/kg bb tidak menyebabkan penurunan mikronukleus.Kata Kunci: Rimpang Kencur (Kaempferia galanga L), Antiklastogenik.

Molecular Regulation of Noradrenaline in Bovine Corpus Luteum ANOM BOWOLAKSONO
HAYATI Journal of Biosciences Vol. 19 No. 2 (2012): June 2012
Publisher : Bogor Agricultural University, Indonesia

Noradrenergic stimulation increases progesterone, oxytocin and prostaglandins in the bovine luteal tissue. Better understanding of noradrenaline (NA) role in bovine the corpus luteum (CL) can advance our current knowledge on the regulatory mechanism of CL function. The present study was conducted to explore the source of noradrenaline and further to investigate whether nerve growth factor (NGF), insulin like growth factor 1 (IGF1) and transforming growth factor b1 (TGFb1) influence the expression of dopamine-b-hydroxylase (DBH), biosynthetic enzyme of NA in cultured bovine luteal cells. Corpora lutea were collected and classified into stages of early, developing, mid, late, and regressed. Messenger RNA (mRNA) and protein expression of DBH were studied throughout the estrous cycle. Additionally, DBH protein expression was examined in cultured mid luteal cells after tumour necrosis factor alpha/interferon gamma (TNFa/IFNg)-induced apoptosis or after treatment with NGF, IGF1, and TGFb1. DBH mRNA and protein expressions were detected throughout the cycle without significant changes in the protein level while mRNA showed a decrease at the developing stage (P < 0.05). Interestingly, NGF, IGF1, and TGFb1 increased DBH expression in cultured luteal cells (P < 0.05). The overall findings suggest non-neural source of noradrenaline in the bovine CL which appears to be regulated by NGF, IGF1, and TGFb1 indicating intraluteal molecules play an important and unrecognized role in the CL function.

Effect of Ethylene Glycol on Structural Integrity at Each Stage of Preantral Follicle Development Post Vitrification of Rat Ovary-Histological Analysis Nova Anita; Abinawanto Abinawanto; Ahmad Aulia Jusuf; Anom Bowolaksono; Huriyah Adani Saoemi
HAYATI Journal of Biosciences Vol. 28 No. 4 (2021): October 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.4.304-311

The structure of follicular tissue affects the ability to maintain the structural integrity of follicles against cryoinjury post-vitrification. Histological analysis was conducted on the structural integrity of each stage of preantral follicles post-vitrification using 7.5% and 15.0% doses of ethylene glycol (EG), and ovarian sections with HE staining were observed using an Olympus CX21 microscope connected to Optilab 3.0 lens and Image Raster software. Analysis was conducted on the ovarian cortex in the tracing line area using polygon measure tools to obtain follicle density (follicles/mm2) and follicle index (%) data. The result showed that the EG group 7.5% (KP1) increased follicle density compared to the vitrified group (KKV) in primordial (15.83±1.77) and primary (22.94±8.51) stages. Meanwhile, KP2 (EG 15%) was in primordial (41.92±6.45), primary (11.69±1.95), secondary (33.48±3.63), and tertiary (5.93±0.69) stages. KP1 increased grade 3 follicle index compared to KKV in primary (27.66±2.34), secondary (32.41±6.99), and tertiary (25.00±5.00) stages. Meanwhile, KP2 was in primary (26.87±6.68) and tertiary (25.00±5.00) stages. Both doses of 7.5% and 15.0% EG were able to maintain structural integrity at certain stages of preantral follicles. Secondary and tertiary follicles are the best stages in maintaining grade 3 follicular integrity with the addition of 7.5% EG.

The honey solution effect as a natural cryoprotectant on sperm quality of botia, Chromobotia macracanthus Bleeker 1852 Abinawanto Abinawanto; Siti Z Musthofa; Retno Lestari; Anom Bowolaksono
Jurnal Iktiologi Indonesia Vol 20 No 3 (2020): October 2020
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v20i3.528

The purpose of study was to obtain the best combination of natural cryoprotectant (honey solution) and synthetic cryoprotectant (methanol) on the quality of spermatozoa Chromobotia macracanthus Bleeker 1852, after freezing at -80 ℃ for 48 hours. The combination of 10% methanol with various concentrations of honey solution (0%, 0.1%, 0.3%, 0.5%, 0.7% and 0.9%) was tested in this study. Ringer's solution was used as an extender. The diluted sperm was then equilibrated for 25 minutes at 4 ℃, then frozen at -80 ℃ for 48 hours. The sperm was then thawed at 40 ℃ for 13 seconds. Viability, motility and percentage of fertilization were evaluated. The results of the analysis of variance showed that the combination of 10% methanol with several concentrations of honey solution had a significant effect (P <0.05) on the viability of spermatozoa and the percentage of fertilization, but had no effect on sperm motility (P> 0.05). The combination of 0.1% honey solution and 10% methanol showed the highest percentage of both motility (89.4 ± 5.45%), viability (85.75 ± 4.79%), and the percentage of fertilization (98.55 ± 1.69%). %). The conclusion of this study is that 0.1% honey solution combined with 10% methanol in Ringer's solution is the best cryoprotectant for C. macracanthus spermatozoa stored at -80 ℃ for 48 hours. Abstrak Penelitian ini bertujuan untuk mendapatkan kombinasi terbaik antara krioprotektan alami (larutan madu) dan krioprotektan sintetik (metanol) terhadap kualitas spermatozoa Chromobotia macracanthus Bleeker 1852, pasca penyimpanan pada suhu -80 ℃ selama 48 jam. Kombinasi metanol 10% dengan berbagai konsentrasi larutan madu (0%, 0,1%, 0,3%, 0,5%, 0,7% dan 0,9%) diuji dalam penelitian ini. Larutan Ringer digunakan sebagai ekstender. Sperma yang telah diencerkan, kemudian diekuiliberasi selama 25 menit pada suhu 4 ℃, kemudian disimpan beku pada suhu -80 ℃ selama 48 jam. Sperma kemudian dicairkan pada suhu 40 ℃ selama 13 detik. Viabilitas, motilitas dan persentase fertilisasi dievaluasi. Hasil uji analisis sidik ragam menunjukkan bahwa kombinasi metanol 10% dengan beberapa konsentrasi larutan madu berpengaruh secara signifikan (P<0,05) terhadap viabilitas spermatozoa dan persentase fertilisasi, tapi tidak berpengaruh terhadap motilitas spermatozoa (P>0,05). Kombinasi larutan madu 0,1% dan metanol 10% menunjukkan persentase tertinggi baik motilitas (89,4 ± 5,45%), viabilitas (85,75 ± 4,79%), maupun persentase fertilisasi (98,55 ± 1,69%). Kesimpulan penelitian ini adalah bahwa larutan madu 0,1% yang dikombinasikan dengan metanol 10% dalam larutan Ringer merupakan krioprotektan terbaik bagi spermatozoa C. macracanthus yang disimpan pada suhu -80 ℃ selama 48 jam.

Sperm DNA Fragmentation and Chromatin Maturation of Prepared Sperm with ALA Supplementation: The Effect of Embryo Development Quality silvia werdhy lestari; aucky hinting; Petrus Supardi; anom Bowolaksono; asmarinah asmarinah
Journal of Global Pharma Technology Volume 11 Issue 09: (2019) September 2019
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract: This study was aimed to determine the differences in embryo quality between spermatozoa with good or poor of DNA fragmentation index and chromatin maturation, through spermatozoa preparation with the addition of ALA. This experimental study compared the quality of embryos that fertilized by spermatozoa with DNA fragmentation and chromatin maturation, with and without ALA supplementation. In this study semen samples from 20 patients were collected, which were then prepared and examined for DNA fragmentation index (DF) and chromatin maturation (CM). Then the sample was divided into 4 groups, namely the good DF - good CM, good DF - poor CM, poor DF - good CM and poor CM - poor CM, further divided again with and without treatment (ALA supplementation). After injection of spermatozoa into the oocyte, the development of embryo was observed in each group. In the group without treatment, significant differences in embryo quality were obtained between groups of good DF - good CM with poor DF - good CM, good DF - good CM with poor DF - poor CM, good DF - poor CM with poor DF - good CM and good DF - poor CM with poor DF - poor CM (p <0.05). In the treatment group, there were not significant difference in the quality of the embryo between treatment groups, namely with or without ALA, in the group with good or poor DF, and with good or poor CM (p> 0.05). Embryo fertilized by spermatozoa with good or poor DF and with good or poor CM, with the supplementation of ALA, the quality was not significantly better than without the supplementation of ALA. Keywords: spermatozoa DNA fragmentation, spermatozoa chromatin maturation, ALA, in vitro fertilization, embryo quality

Growth Characteristics of Chikungunya Virus Isolate from Indonesia in Various Human Cell Lines in vitro Oktaviani Naulita Turnip; OKTAVIANI N. TURNIP; RAHMA F. HAYATI; RIZKA ALAWIYAH; BENEDIKTUS YOHAN; DIONISIUS DENIS; ANOM BOWOLAKSONO; AMIN SOEBANDRIO; R. TEDJO SASMONO
Microbiology Indonesia Vol. 13 No. 1 (2019): March 2019
Publisher : Indonesian Society for microbiology

Chikungunya (CHIK) fever, a febrile illness caused by Chikungunya virus (CHIKV) infection, is one of mosquito-borne viral diseases affecting people living in the tropical and subtropical regions in the world. The pathogenesis of the disease is yet to be completely unraveled, and research on CHIK has been conducted by employing various methods, including using cell lines to investigate the biological characteristics of CHIKV in vitro. To assess the suitability of human cell line model for CHIK study, various human cell lines including A549, Huh7, and HepG2 were infected with CHIKV and assayed for their susceptibility to infection. The MTT and plaque assay methods were performed to measure cell viability and virus growth kinetics, respectively. Fluorescence-activated Cell Sorting (FACS) and immunofluorescence assay were performed to measure the proportion of infected cells in the system and their morphological visualization. Both A549 and Huh7 human cell lines showed stable high cell viability upon infection while CHIKV growth kinetics were significantly lower in these cells compared to Vero-CCL81, a monkey cell line that is routinely used in other arboviruses research. Interestingly, we observed significantly different results in HepG2 human cell line, in which cell viability and CHIKV growth kinetics were significantly higher. FACS and immunofluorescence assay confirm the higher infection rate of CHIKV in HepG2 than A549 human cell line. We concluded herethat human hepatocytes HepG2 cell line was susceptible to Asian Genotype of CHIKV and proposed as an alternative cell for the in vitro CHIKV studies to the commonly used A549 and Vero cells.

Î±-Mangostin Effectively Inhibits Chikungunya Virus Replication in HepG2 Cells Moudy Soraya; Justus Sievers; Dionisius Denis; Anom Bowolaksono; R. Tedjo Sasmono
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1187-1193

Chikungunya virus (CHIKV) is an arthropod-transmitted Alphavirus endemic to countries in Africa and Asia, including Indonesia, which causes debilitating arthralgia which can last several years. The rapid spread of CHIKV to new areas makes the discovery of antiviral agents a high priority. Î±-mangostin is a xanthone from mangosteen (Garcinia mangostana) pericarp and has antiviral activity against Hepatitis C and Dengue viruses. We investigated the antiviral activity of Î±-mangostin against CHIKV in HepG2 cells in pre-, post- and combination treatments compared to the common antiviral medicine ribavirin, as well their cytotoxicity. Our results show dose-responsive reductions in viral titer in all treatment regimes, with post- and combination treatments being more effective than pre-treatment only (IC50 = 7.79, 5.99 and 6.39 ÂµM, respectively), but with poor specificity (SI = 1.39, 1.81 and 1.70, respectively) compared to ribavirin. Neither compound showed a direct virucidal effect. These results suggest Î±-mangostin effectively inhibits CHIKV replication in this cell line.

Hormonal and Laboratory Profile of Polycystic Ovarian Syndrome (Pcos) Women Visiting Fertility Center in Indonesia Sundari, Ayu Mulia; Wulandari, Tri Aprilliana; Prakoso, Nurul Muhammad; Dwiranti, Astari; Abinawanto; Boediono, Arief; Funahashi, Hiroaki; Bowolaksono, Anom
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 18 No 1 (2024): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jpm.v18i1.7546

Our study aims to scrutinize the hormonal and laboratory profile of women with PCOS visiting a fertility center in Indonesia. A computerized database of 120 PCOS women and 120 controls was retrospectively reviewed and analyzed. According to analysis, the concentration of AMH and basal LH were significantly more elevated in the PCOS group than in the control group (6.77 vs. 2.05, P<0.001; and 6.8 vs. 5.3, P<0.001, respectively). The level of basal FSH was significantly lower in the PCOS group (5.83 vs. 7.86; P<0.001). After an IVF cycle, PCOS women had significantly higher retrieved oocytes and mature oocytes compared to controls (18 vs. 8, P<0.001; 12 vs. 6, P<0.001). The number of fertilizations, cleavages, and top-quality cleavages were also higher in the PCOS group than in the control group (10 vs. 4, P<0.001; 10 vs. 4, P<0.001; 4 vs. 2, P<0.001). At the blastocyst stage, patients with PCOS had a higher number of blastocysts and top-quality blastocysts than control (9 vs. 4, P<0.001); 4 vs. 1, P<0.01). However, the clinical pregnancy was significantly higher in the control group than in the PCOS group (52.6% vs. 16.7%; P = 0.045).

Effect of Oral Administration of Dehydroepiandrosterone on PCOS-Like Phenotype of Female C57BL/6 Mice Sundari, Ayu Mulia; Prakoso, Nurul Muhammad; Dwiranti, Astari; Abinawanto, Abinawanto; Boediono, Arief; Funahashi, Hiroaki; Bowolaksono, Anom
Jurnal Sain Peternakan Indonesia Vol 19 No 2 (2024)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.19.2.111-116

We aim to evaluate the efficacy and optimal dose of orally administered DHEA in the PCOS mice model by assessing their ovarian morphology and serum FSH, LH, and testosterone levels. Female C57BL/6 mice were divided into a control group (n=5, received daily injections of 0.2 ml sesame oil) and an experimental group, which was further classified into 1) D-45 group (n=5), 2) D-60 (n=5), and 3) D-90 group (n=5) that were treated with 45, 60, and 90 mg/Kg body weight of oral DHEA. After modelling, mice in the control group had a regular estrous cycle, while mice in all experimental groups had a disturbed estrous cycle. Ovarian histology showed several growing follicles and some corpora lutea (CL) in the control, D-60, and D-90 groups. However, some large antral follicles and decreased CL were observed in the D-45 group. Serum FSH was significantly lower in the D-45 group than in the control group (3.73 ± 0.12 vs. 5.28 ± 0.31 mIU/mL, P<0.01), but D-60 and D-90 groups had a similar FSH level to the control group (P>0.05). The serum level of LH and testosterone were significantly elevated in the D-45 group than in the control group (2.52 ± 0.43 vs. 1.30 ± 0.33 mIU/mL, P<0.01 and 1.80 ± 0.32 vs. 1.24 ± 0.23 ng/mL, P<0.01, respectively). Still, the level of LH and testosterone in the D-60 and the D-90 groups was comparable to the control group (P>0.05). Our study suggests that oral administration of DHEA is efficacious in establishing PCOS-like phenotype in mice with the suggested optimal dosage of 45 mg/Kg body weight.

Assessment of potential cellular toxicity of methylglyoxal on primary human epidermal keratinocytes Prakoso, Nurul Muhammad; Sundari, Ayu Mulia; Fadhilah; Abinawanto; Dwiranti, Astari; Bowolaksono, Anom
Current Research on Biosciences and Biotechnology Vol. 6 No. 2 (2025)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2025.6.2/JYICDMXO

Skin is the outermost layer of the human body, which is important to prevent damage by infection, ultraviolet radiation, and toxic materials. The impermeable barrier of the skin has to be maintained via skin regeneration to preserve its essential function. However, skin regeneration declines due to decreased cell proliferation during skin aging. In this study, we investigated the role of methylglyoxal (MGO) as a precursor of advanced glycation end-products (AGE), which have been reported as an emerging threat of early aging in the skin. Previous studies reported a reduction in survival of the HaCaT cell line exposed to 500 µM MGO. However, studies on primary keratinocytes are still limited. We evaluated the viability and proliferation of primary human epidermal keratinocytes (HEKa) under the exposure of MGO. We also employed H2DCFDA assay to quantify the generation of intracellular ROS due to MGO. Our study revealed a biphasic response in which lower doses of MGO (25 and 50 µM) increased viability, while the cellular toxicity began at higher concentrations of 100, 200, and 300 µM. The proliferation assay also suggests an overall reduction of primary epidermal keratinocytes exposed to 100 and 200 µM MGO after 24 and 48 hours of culture. Increasing ROS concentrations were also observed in cells treated with 100 and 200 µM MGO. Our data suggested that a lower concentration of MGO was required to induce cellular toxicity in primary human epidermal keratinocytes compared to the HaCaT cell line reported by another study, indicating different sensitivity to MGO.

Co-Authors Abinawanto Abinawanto Abinawanto Ahmad Aulia Jusuf Amin Soebandrio Amira, Qanita Hana Arief Boediono Asmarinah Asmarinah Aucky Hinting BENEDIKTUS YOHAN David Kusmawan Dionisius Denis DIONISIUS DENIS Dwiranti, Astari Fadhilah Fadilah Firmansyah, Ardi Funahashi, Hiroaki Gustina, Ira Heatubun, Charlie D. Huriyah Adani Saoemi Ismail Jatna Supriatna Justus Sievers Liling, Irman Moudy Soraya Mufti Petala Patria Nova Anita Oktaviani Naulita Turnip Pambudi, Sabar Perdana, Silvia M. Petrus Supardi Prakoso, Nurul Muhammad R. Tedjo Sasmono R. TEDJO SASMONO RAHMA F. HAYATI Rajagukguk, Selly Setiati Retno Lestari RIZKA ALAWIYAH S., Putri Irwanti Setiorini, Setiorini silvia werdhy lestari Siti Z Musthofa Sundari, Ayu Mulia Utomo, Doddy Irawan Setyo Wulandari, Tri Aprilliana

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