Article Per Year (5 Year)
p-Index From 2021 - 2026
0.562
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Jurnal Teknosains Indonesian Journal of Cancer Chemoprevention
Kastian, Ria Fajarwati
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Anti-SARS-CoV-2 Activity of Andrographis paniculata (Burm.f.) Nees Extract via Inhibition of Spike-mediated Syncytia Formation in HEK293T Cell Model Prasetyaningrum, Pekik Wiji; Kastian, Ria Fajarwati; Novianti, Metta; Santoso, Adi; Septisetyani, Endah Puji
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.996-1006

Abstract

The resurgence of COVID-19 endemic cases at the end of 2023 has underscored the need for effective treatments. Some severe cases of COVID-19 are often characterized by the formation of multinucleated syncytial pneumocytes in the lungs. Therefore, our study aimed to explore the potential of Andrographis paniculata (Burm. f) Nees as an antivirus against SARS-CoV-2, which involves syncitia formation. We utilized the non-toxic concentrations of A. paniculata extract on HEK293T cells determined by MTT assay, which were 1 μg/ml (cell viability 97.96%) and 10 μg/ml (cell viability 95.24%) for further assays. First, we conducted a pseudovirus cellular entry assay as a model of SARS-CoV-2 infection in HEK293T cells expressing hACE2/TMPRSS2. The HEK293T cells were co-transfected with plasmids expressing hACE2 and TMPRSS2, then infected with pseudotyped spike*∆G-GFP rVSV with or without A. paniculata extract. The internalized pseudovirus would trigger GFP expression as a reporter of the infected cells. Next, we performed a syncytia assay by transfecting HEK293T cells with hACE2, TMPRSS2, and SARS-CoV-2 spike expression vectors to induce syncytia formation as a model of intercellular viral transmission. As the results, 10 μg/mL of the extract significantly lowered the number of SARS-CoV-2 pseudovirus-infected cells by 54.69% (P = 0.02) and spike-mediated syncytia formation by 42.39% (P<0.001). In conclusion, our results suggested that A. paniculata has a potential antiviral activity against SARS-CoV-2 by hindering virus infection and cell-to-cell transmission.
Viability of bhk-21 fibroblast cells toward acrylic denture bases after reinforced by natural fibers Prawesthi, Endang; Tetelepta, Marzia Magdalena; Heldayani, Heldayani; Kastian, Ria Fajarwati; Septisetyani, Endah Puji
Jurnal Teknosains Vol 14, No 1 (2024): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.90278

Abstract

The use of acrylic denture bases in the oral cavity requires biocompatibility. This study investigated the viability of BHK-21 fibroblast cells after treatment with an acrylic denture base modified using natural fibers. Ramie and banana stem fibers were used as cost-effective alternatives to synthetic fibers. The study involved 42 acrylic resin specimens (10 mm diameter, 2 mm thickness) divided into groups: resin without fibers, 0.5%, 1.5%, and 2.5% ramie fibers, and 0.5%, 1.5%, and 2.5% banana stem fibers. The resin was incubated with cell culture media at 37°C for 7 days. Cytotoxicity testing using the MTT method revealed that all treatment groups had cell viability exceeding 70%, meeting ISO 10993-5 standards. No significant differences in cell viability were observed between the treatment groups and the control (media without specimens). Additionally, adding 0.5%, 1.5%, and 2.5% ramie fibers did not affect BHK-21 cell viability compared to the resin-only control, while adding banana stem fibers increased cell viability compared to the control (P = 0.035; P = 0.021; and P = 0.011). In conclusion, increasing the concentration of natural fibers in acrylic denture bases did not negatively impact fibroblast cell growth.
Analysis of SARS-CoV-2 spike-Induced Syncytia with Lifeact-GFP as Biosensor Using High-Content Screening Instrument for Automated Syncytia Counting Fauziah, Dita; Septisetyani, Endah Puji; Yerizel, Eti; Kastian, Ria Fajarwati
Indonesian Journal of Cancer Chemoprevention Vol 15, No 2 (2024)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev15iss2pp127-136

Abstract

SARS-CoV-2 is believed to cause cytopathic effects in forming multinucleated cells, known as syncytia. Syncytia due to SARS-CoV-2 infection found in lung tissue samples of COVID-19 patients represents a case of COVID-19 with a poor prognosis. Therefore, it is very important to study the mechanism of syncytia formation and to test candidate materials that can inhibit the occurrence of syncytia and potentially be applied in the treatment or prevention of COVID-19. Since syncytia counting and analysis are time-consuming, we utilized a high-content screening (HCS) instrument in this study to automate syncytia analysis. We used 293T cells transfected with plasmids to express the SARS-CoV-2 spike, human angiotensin-converting enzyme-2 (hACE-2), and a plasmid encoding lifeact-GFP as an F-actin biosensor to facilitate syncytia analysis using the HCS instrument. In this study, syncytia analysis was carried out using HCS software. The HCS application categorizes cells as multi-nuclei by counting the number of cell nuclei stained with DAPI in cells that emitted green fluorescence due to lifeact-GFP expression. Syncytia analysis is time-consuming because of the calculation of the number of syncytia formed in a confluent cell monolayer culture. Hopefully, utilizing the HCS platform can accelerate the test of syncytia inhibition after various treatments using test compounds.Keywords: 293T cells, high-content analysis, SARS-CoV-2, spike, syncytia.
Co-Authors Adi Santoso Endah Puji Septisetyani, Endah Puji Eti Yerizel Fauziah, Dita Heldayani Heldayani, Heldayani Marzia Magdalena Tetelepta Novianti, Metta Prasetyaningrum, Pekik Wiji Prawesthi, Endang