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All Journal Journal of Food and Pharmaceutical Science Journal of Food and Pharmaceutical Sciences Jurnal Karya Generasi Sehat RESLAJ: Religion Education Social Laa Roiba Journal
Rumiyati
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Religion Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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Application of Real-time PCR with Mitochondrial D-Loop Specific Primers for Halal Authentication: Identifying Bovine Adulteration in Meatball Products Arini, Rien Larasati; Rohman, Abdul; Rumiyati
Journal of Food and Pharmaceutical Sciences Vol 11, No 3 (2023): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.8930

Abstract

This study aimed to develop an analytical method using Real-time PCR and a specific primer to analyze bovine DNA in meatballs, addressing the issue of non-halal meat being used in halal meatballs. Real-time PCR enables rapid, specific, and sensitive detection, allowing for qualitative and quantitative identification of species in processed products. The research involved designing specific primers for bovine DNA using IDT software, followed by DNA isolation and testing for various parameters such as specifications, linearity, limit of detection, efficiency, and repeatability. The results demonstrated that the primer D-Loop 922 (forward: 5-ATTACCATGCCGCGTGAA-3', Reverse: 5'-GATGAGATGGCCCTGAAGAAA-3'), designed and tested in silico using Primer-BLAST software from NCBI, effectively identified bovine DNA in both fresh meat and meatballs at an optimum annealing temperature of 59.5°C. The real-time PCR method utilizing the D-loop 922 primer successfully amplified bovine DNA in both bovine samples and bovine meatballs at a minimum concentration of 1 ng, with coefficients of variation (CV) of 0.20% for bovine DNA and 0.22% for bovine meatballs. Consequently, the D-loop 922 primer met the testing criteria and can be utilized to authenticate the halal status of meatball products, supporting the implementation of Law No. 33 of 2014 regarding halal certification.
HUBUNGAN KADAR HEMOGLOBIN DENGAN INDEKS MASSA TUBUH PADA PASIEN JIWA DI RUMAH SAKIT JIWA SAMBANG LIHUM Rumiyati; Ahmad Muhlisin; Akhmad Muntaha; Aima Insana
Jurnal Karya Generasi Sehat Vol. 2 No. 1 (2024): Edisi Juni 2024
Publisher : Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jkgs.v2i1.119

Abstract

Gangguan jiwa adalah sekelompok gejala yang ditandai dengan perubahan pikiran, perasaan, dan perilaku seseorang yang menimbulkan disfungsi dalam menjalankan aktivitas sehari-hari. Hemoglobin merupakan salah satu senyawa dalam sel darah merah yang berfungsi mengangkut zat oksigen ke dalam sel-sel tubuh. Kadar hemoglobin dalam darah merupakan indikator laboratorium yang digunakan untuk menetapkan prevalensi anemia. Penelitian ini bertujuan untuk mengetahui hubungan kadar Hemoglobin dengan Indeks Massa Tubuh pada pasien jiwa. Metode yang digunakan Cross Sectional dengan tehnik sampling Accidental. Penelitian dilaksanakan tanggal 08 sampai 31 Agustus 2023. Populasi penelitian adalah pasien jiwa yang di rawat di Rumah Sakit Jiwa Sambang Lihum. Responden sebanyak 30 orang dengan Teknik pengambilan Total Sampling. Hasil penelitian kadar Hemoglobin pada pasien jiwa didapat nilai dibawah normal 15 orang (50 %), nilai normal 13 orang (43,3%), nilai diatas normal sebanyak 2 orang (6,7 %). Hasil pengukuran Indeks Massa Tubuh didapat hasil kurus sebanyak 7 orang (23,3 %), Normal sebanyak 18 orang (60,0 %), gemuk sebanyak 5 orang (16,7 %). Hasil analisa uji statistik Pearson didapatkan nilai pearson correlation 0.396 korelasi itu menunjukkan korelasi rendah. Nilai p value 0.030 pada Sig.2 tailed disimpulkan ada hubungan antara kadar Hemoglobin dengan Indeks Massa Tubuh pada pasien jiwa. Penelitian ini dapat memberikan informasi tambahan bagi tenaga medis dalam mengelola pasien jiwa yang memiliki masalah Kesehatan terkait kadar Hemoglobin dan Indeks Massa Tubuh. Kata kunci : Hemoglobin, Indeks Massa Tubuh, Pasien Jiwa
Application of Real-time PCR with Mitochondrial D-Loop Specific Primers for Halal Authentication: Identifying Bovine Adulteration in Meatball Products Arini, Rien Larasati; Rohman, Abdul; Rumiyati
Journal of Food and Pharmaceutical Sciences Vol 11, No 3 (2023): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.8930

Abstract

This study aimed to develop an analytical method using Real-time PCR and a specific primer to analyze bovine DNA in meatballs, addressing the issue of non-halal meat being used in halal meatballs. Real-time PCR enables rapid, specific, and sensitive detection, allowing for qualitative and quantitative identification of species in processed products. The research involved designing specific primers for bovine DNA using IDT software, followed by DNA isolation and testing for various parameters such as specifications, linearity, limit of detection, efficiency, and repeatability. The results demonstrated that the primer D-Loop 922 (forward: 5-ATTACCATGCCGCGTGAA-3', Reverse: 5'-GATGAGATGGCCCTGAAGAAA-3'), designed and tested in silico using Primer-BLAST software from NCBI, effectively identified bovine DNA in both fresh meat and meatballs at an optimum annealing temperature of 59.5°C. The real-time PCR method utilizing the D-loop 922 primer successfully amplified bovine DNA in both bovine samples and bovine meatballs at a minimum concentration of 1 ng, with coefficients of variation (CV) of 0.20% for bovine DNA and 0.22% for bovine meatballs. Consequently, the D-loop 922 primer met the testing criteria and can be utilized to authenticate the halal status of meatball products, supporting the implementation of Law No. 33 of 2014 regarding halal certification.
Implementasi Pembelajaran TIK di Kelas V MIs Sirojul Falah 01 Kec. Parung Bogor Agus Suryana; Dede Hendro Gunawan; Eka purwaningsih; Rumiyati
Reslaj: Religion Education Social Laa Roiba Journal Vol. 7 No. 2 (2025): RESLAJ: Religion Education Social Laa Roiba Journal
Publisher : Intitut Agama Islam Nasional Laa Roiba Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47467/reslaj.v7i2.5267

Abstract

The study aims to analyze and describe the implementation of ICT learning for grade V students at MIS Sirojul Falah Parung, Bogor. The qualitative descriptive approac used with interviews, and observations to collect the data. Then in analyzing using the Milles and Huberman model, namely data reduction, data presentation, data analysis, and drawing conclusions. The results of the study show that the implementation of ICT learning for grade V students at MIS SIROJUL FALAH Parung, Bogor Regency has three stages in learning, namely planning, implementation, evaluation and follow-up. The implementation of ICT learning at MIS SIROJUL FALAH Parung Bogor still has many shortcomings. First, in terms of subject names, it still uses the name of the ICT subject which should have been changed to Informatics. The two curriculums used still use KTSP which should be the Independent Curriculum and the RPP used is not in line with the curriculum used. Third, the scope of the material taught still has many shortcomings where the material taught still contains old material that has not changed and is still around ICT which should be ICT material in MI or elementary school that is taught must contain Science, technology and mathematics. Fourth, computer facilities are still insufficient to meet the learning needs of students.
Co-Authors Abdul Rohman Agus Suryana Ahmad Muhlisin Aima Insana akhmad muntaha Arini, Rien Larasati Dede Hendro Gunawan Eka Purwaningsih