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Pamungkas, D.
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Motility characterization of albumin sexed spermatozoa in two different diluents and additional antioxidant Ratnawati, D.; Luthfi, M.; Pamungkas, D.; Affandhy, L.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 4 (2020): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.4.277-286

Abstract

The purpose of this study was to characterize spermatozoa motility of sexed semen (X and Y) using albumin by diluents and antioxidant treatments. The material used in this study was fresh semen of Ongole Crossbreed (OC) bull with progressive motility >70%. The sperm sexing methodology used albumin gradient of 5%, 10%, and 15%. The diluents used were CEP-2 and andromed with or without the addition of 1mM Glutathione as an antioxidant. The sexed semen was made into liquid semen and stored at 3-5°C. The motility was observed at day 0 (H0) and day 5 (H5). Motility was analyzed using SCA v.2.1. The parameters measured were total motility, progressive motility, VCL, VSL, VAP, LIN, STR, WOB, ALH, BCF and sperm hyperactive. The experimental applied was the factorial pattern 2 x 2. The first factor was the type of diluents (CEP-2 and Andromed), and the second factor was additional glutathione (with or without). Data was analyzed by general linear model of SPSS-IBM 24 program. Sexed semen with CEP-2 diluents showed better motility of spermatozoa than andromed in the upper layers (spermatozoa X) and lower layers (spermatozoa Y). The CEP-2 diluent had a big role to maintain motility, progressive motility and velocity spermatozoa during cold storage. The addition of Glutathione 1mM could support the motility of sexed spermatozoa at cold storage, especially for LIN, STR, and WOB values. 
Association of GHR|AluI gene polymorphism with body weight parameters in Grati-PO cattle Hartati, H.; Hapsari, A.A.R.; Soewandi, B. D. P.; Anwar, S.; Rahmadani, S. P.; Aryogi, A.; Pamungkas, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 46, No 4 (2021): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.46.4.272-281

Abstract

The growth hormone receptor (GHR) gene is member of the class I cytokine receptor family that important in growth regulation including in cattle. This study aimed to analyze the association between GHR|AluI gene with body weight, parameters in Grati-PO cattle population. A total of 304 blood samples were collected from the experimental station at the Beef Cattle Research Station, which were also equipped with 304 phenotypic data. DNA was extracted using a zymo extraction kit. Detection of of the polymorphism of GHR gene using PCR-RFLP method with AluI restriction enzymes. polymorphism indicators include genotype frequency, allele frequency, Hardy-Weinberg Equilibrium, observed heterozygosity (Ho), expected heterozygosity (He), polymorphism information content (PIC) and number of effective alleles (Ne). Association analysis was carried out using a linear mixed model. The performance of birth weight, weaning weight, yearling weight and 18 months weight were 25.4 + 0.2 kg; 113.8 + 2.3 kg; 159.9 + 2.7 kg and 213.7 + 3.6 kg, respectively. The result of association analysis was significant (P<0.05) on weaning weight, yearling weight and 18 months weight. It could be concluded that the GHR gene has potential to be a candidate genetic marker for growth traits in Grati-PO cattle.
Co-Authors Affandhy, L. Aryogi, A. Hapsari, A.A.R. Hartati, H. Luthfi, M. Rahmadani, S. P. Ratnawati, D. S. Anwar Soewandi, B. D. P.