Hawaiian papaya fruit has nutritional content that can nourish the human body. One of the nutrients contained in this fruit is vitamin C. The benefit of vitamin C is to help the absorption of iron, thus avoiding anemia. The purpose of the study was to determine and analyze the levels of vitamin C in Hawaiian papaya fruit. The method used in the study was to use qualitative and quantitative analysis tests with UV-Vis spectrophotometric devices conducted at the Sucofindo Laboratory in Surabaya in September 2022-January 2023. The results of the qualitative analysis test state that Hawaiian papaya fruit has vitamin C content, while the results of the quantitative analysis test state that the level of vitamin C content in papaya is 726 mg/100 g. The conclusion of this study is that Hawaiian papaya fruit can be consumed by the community because the level of vitamin C content in this fruit can meet nutritional needs in the body, especially can increase hemoglobin levels in the blood.