Muthanna Hamid Hassan
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Determination of Probiotic Potential and Anticancer Activity of Lactobacillus Isolated from Cheese and Yogurt Muthanna Hamid Hassan
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12120

Abstract

In this study, microorganism was isolated from cheese and yogurt, cultured on specific culture media, thenrecognized molecularly through 16S rDNA f quality sequencing. The supernatant of Lactobacillus strainscontained probiotic activity such as anticancer effects against cancer cell lines such as HeLa, RD and normalcell line (REF )cells and antibacterial. The cytotoxicity effects were assessed by MTT stain and apoptosis inmitochondria. The supernatant showed had anticancer activity against the tested cell lines (P< 0.05) with nosignificant cytotoxic effects against normal cell line .
Effect of Exotoxin a isolated from Pseudomonas Aeruginosaagainsthuman Cancer Cell Lines Muthanna Hamid Hassan
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16972

Abstract

In this study, the presence of Pseudomonasaeruginosawas investigated from many clinical samples, andthen about 54 isolates were obtained, diagnosed with all different diagnostic methods, and then the bacterialisolates produced for ExotoxinA were detected by the ELISA method and then the most productive isolatewas chosen for purification by means ofPrecipitation with ammonium sulphate and ion-exchange columnand detection of molecular weight was (M.W 65.03KD).The competent isolation tested its toxicity againsttwo cancer cell linesHeLa and PC3 which were an inhibition ratio 69.1% and 61.6% respectively in highconcentration.
Effect of Exotoxin a Isolated from Pseudomonas Aeruginosa Against Human Cancer Cell Lines Muthanna Hamid Hassan
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15448

Abstract

In This study, the presence of Pseudomonas aeruginosa was investigated from many clinical samples, and then about 54 isolates were obtained, diagnosed with all different diagnostic methods, and then the bacterial isolates produced for Exotoxin A were detected by the ELISA method and then the most productive isolate was chosen for purification by means of Precipitation with ammonium sulphate and ion-exchange column and detection of molecular weight was (M.W 65.03KD).The competent isolation tested its toxicity against two cancer cell lines HeLa and PC3 which were an inhibition ratio 69.1% and 61.6% respectively in high concentration