In this study, the presence of Pseudomonasaeruginosawas investigated from many clinical samples, andthen about 54 isolates were obtained, diagnosed with all different diagnostic methods, and then the bacterialisolates produced for ExotoxinA were detected by the ELISA method and then the most productive isolatewas chosen for purification by means ofPrecipitation with ammonium sulphate and ion-exchange columnand detection of molecular weight was (M.W 65.03KD).The competent isolation tested its toxicity againsttwo cancer cell linesHeLa and PC3 which were an inhibition ratio 69.1% and 61.6% respectively in highconcentration.
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