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All Journal Indonesian Journal of Biotechnology Majalah Farmaseutik The Avicenna Medical Journal Indonesian Journal of Pharmacology and Therapy
Pamungkas Bagus Satriyo
Department Of Pharmacology And Therapy, Faculty Of Medicine, Public Health, And Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Acute Oral Toxicity Test of Antihypertensive Polyherbal Preparations Containing Allium sativum Curcuma aeruginosa & Amomi fructus Herzan Marjawan; Woro Rukmi Pratiwi; Dwi Aris Agung Nugrahaningsih; Eti Nurwening Sholikhah; Pamungkas Bagus Satriyo
Majalah Farmaseutik Vol 18, No 4 (2022): in press
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v18i4.79299

Abstract

Hypertension is a major factor causing atherosclerotic cardiovascular disease, heart failure, stroke, and kidney failure. Polyherbal preparations containing garlic (Aliium sativum), temu ireng (Curcumae aeruginosae) and cardamom (Amomi fructus) have been widely used to treat hypertension. Despite widely used in community, its safety has not been evaluated. This study aimed to evaluate the single dose oral safety of the polyherbal. The acute oral toxicity test was done using fixed dose methods. Single dose of the polyherbal was administered to female Wistar rats. The clinical examination was done after administration of the polyherbal and continued until the 14th day to check for symptoms of toxicity, changes in body weight. On day 15, the animal was sacrificed and histopathological examination was conducted. The body weight did not differ between animal that received polyherbal and not received polyherbal. However, there was an increase in body weight in a group that received polyherbal at a dose of 2000 mg/kg. The absolute and relative organs weight was also similar among groups. There were no macroscopic and histopathological changes in kidney, liver, heart, spleen, aorta, and lungs. The polyherbal preparations containing Aliium sativum, Curcumae aeruginosae and Amomi fructus is safe with LD50 >2000-5000 mg/kg in Wistar rats.
Development of nanocomplex mimic‐hsa‐miR‐143‐3p loaded exosome (exo‐miR) to inhibit viability, migration and proliferation of triple‐negative breast cancer Nilasari, Fita; Haryana, Sofia Mubarika; Nugrahaningsih, Dwi Aris Agung; Satriyo, Pamungkas Bagus
Indonesian Journal of Biotechnology Vol 29, No 4 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.92817

Abstract

Breast cancer represents the highest number of cancer cases in Indonesia, with triple‐negative breast cancer (TNBC) being a common subtype (10–15%). MicroRNAs play a role in cancer epigenetics and contributing as core factors to the disease. The expression of miR‐143‐3p have been found to be lower in breast cancer samples from Yogyakarta and Central Java. It is known that miR‐143‐3p functions as a tumor suppressor in breast cancer, and its overexpression corresponds with an increased survival rate. The structure of miRNA is quickly degraded, an enhanced delivery system for miRNA is required. Exosomes are indeed emerging as natural delivery agent. A new approach represents that exosomes will be transfected with mimic‐hsa‐miR‐143‐3p yield an exo‐miR. The research aimed to examine how exo‐miR affects viability, migration, and proliferation using 4T1 cell line. The Exo‐Fect‐based method was used to transfect mimic‐hsa‐miR‐143‐3p into exosomes. The MTT assay, wound healing assay, and colony formation assay were used as functional assay. The MTT assay revealed that 7.5 µL/ 250,000 particles exo‐miR obtained a lower percentage of cell viability (58%) than the control (99.7%). The wound healing assay showed that transfection of 37.5 µL/ 1,250,000 particles exo‐miR was able to suppress migration by the percentage of wound closure (67%) compared to the control (100%). Exo‐miR also had a significant (p < 0.001) effect on colony‐forming abilities, as shown by fewer colonies (32) compared to the control (132). This findings demonstrated that exo‐miR represents a promising targeted approach in cancer therapy.
Balb/C Mice Optimization as Triple Negative Breast Cancer ModelWith 4T1 Cells and 4T1 Cells 3D Culture Inoculation Methods Zafrullah, Umar Farooq; Mubarika Haryana, Sofia; Nugrahaningsih, Dwi Aris Agung; Satriyo, Pamungkas Bagus; Rocca, Matteo
The Avicenna Medical Journal Vol. 6 No. 1 (2025): The Avicenna Medical Journal
Publisher : Faculty of Medicine, UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/avicenna.v6i1.46877

Abstract

Background: Triple Negative breast cancer (TNBC) is a subtype of breast cancer with negative expression of Progesterone Receptor (PR), Estrogen Receptor (ER), and Human Epidermal growth factor Receptor 2 (HER-2). Among other types of breast cancer, TNBC has a high rate of malignancy and recurrence, invasive, distant metastases, and resistance to conventional therapy. An optimal TNBC model is needed for drug discovery research in targeted therapy. However, optimizing the TNBC animal model by inoculating the 4T1 tumor cell line has a number of challenges and it is quite hard to be developed, especially in Indonesia. Methods: This quasi-experiment study used 28 Balb/c mice divided into four groups (7 mice respectively), group A (injected with basal medium), group B (injected with 4T1 cell), group C (injected with 4T1 cell + Geltrex), and group D (injected with 3D Culture 4T1 cell + Geltrex). After being injected, mice were followed up for 35 days. Once a week they had to be observed by measuring body weight and tumor volume. After 35 days, mice were sacrificed. Liver, lung, brain, and mammary gland were analyzed histopathologically with Hematoxylin-eosin (HE). Chi-square statistical analysis was done to compare intergroups. Results: Histopathologically, there was a group that found positive samples of Invasive Ductal Carcinoma (IDC)-like with a percentage of 28. 57% (2 out of 7), namely group C. Meanwhile, group B and D did not find any positive samples of TNBC, but in the mammary tissue, there was inflammation and epithelial hyperplasia. No distant metastases were found in the liver, lungs, and brain, however, there was inflammation in these secondary organs. Statistically, there was no significant difference between treatment groups with p-value = 0.41. Conclusion: The 4T1 cell inoculation method can produce IDC-like on HE with an incidence of 28.75% (2 of 7 individuals) in group C.
Potential targeted therapy: The role of MicroRNAs in breast cancer metastasis via epithelial-mesenchymal transition and cancer stem cell regulation Satriyo, Pamungkas Bagus
Indonesian Journal of Pharmacology and Therapy Vol 2 No 1 (2021)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.1184

Abstract

Abstract In the advancement of breast cancer treatment, metastatic breast cancer is remaining as an incurable disease. It contributes to almost 90% of cancer-related death in breast cancer cases. Epithelial to Mesenchymal Transition (EMT) is a serial change of the epithelial cell to gain the mesenchymal-like phenotype. In cancer, the cells that undergo the EMT lose the adherent junction protein, cell polarity, and gain the invasive phenotype. Recent studies showed that the EMT induces the cancer stem cell-like phenotypes in cancer cells. These cells possess self-renewal ability, and multi-lineage differentiation capacity to generate the new bulk of tumor during cancer distant metastasis. Both EMT and cancer stem cells take responsibility in drug-resistant, and relapse cases in breast cancer. In the last decades, a new type of non-coding RNA, microRNA (miR) shows have an important role in the normal physiological and pathophysiological condition such as cancer. Recent studies revealed that the EMT is regulated by microRNAs. In this review, we discussed the microRNAs regulation on the EMT process through TGF-β, and Wnt signaling pathways in breast cancer. Understanding of microRNA regulation in EMT in breast cancer metastasis gives a chance to explore a new therapy approach to improve the prognosis of breast cancer patients. In addition, we also explored several potential approaches targeting microRNA as a new approach of cancer treatment. Keywords: breast cancer, microRNA, EMT, metastasis, targeted therapy.
Chitosan nanoparticle‐mediated delivery of anti‐miR‐203a‐3p for 4T1 triple‐negative breast cancer Temartenan, Jecklyn Shindy; Fiqri, Hairil; Satriyo, Pamungkas Bagus; Haryana, Sofia Mubarika
Indonesian Journal of Biotechnology Vol 31, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.110535

Abstract

Anti‐miR molecules can suppress specific microRNA (miRNA) functions within critical signaling pathways. Chitosan acts as a delivery system for miRNAs; therefore, encapsulating miR‐203a‐3p is essential for targeted delivery and biological activity. This study investigates the impact of chitosan‐encapsulated anti‐miR‐203a‐3p nanoparticles (CS‐NPs) on the viability, proliferation, and migration of triple‐negative breast cancer (TNBC) 4T1 cells. The nanoparticles were synthe‐ sized using the ionic gelation method in a 5:1 ratio of chitosan to anti‐miR‐203a‐3p, incorporating sodium tripolyphosphate (STPP) as a crosslinker. Characterization was conducted using gel electrophoresis and particle size analysis. Cytotoxicity and cell viability were assessed through the MTT assay, while colony formation and wound healing assays evaluated cell proliferation and migration. The nanoparticles demonstrated an encapsulation efficiency of 89.47% and showed significant inhibitory effects on 4T1 cell proliferation and migration. The MTT results indicate an IC50 value of 2.454 µM, while colony formation analysis revealed that both ½ and IC50 doses significantly reduced colony numbers compared to the control. Similarly, wound healing assays showed notable inhibition of cell migration at ¼, ½, and IC50 concentrations. These findings suggest that anti‐miR‐203a‐3p‐loaded CS‐NPs may offer a promising therapeutic approach to managing aggressive breast cancer subtypes, particularly TNBC.
Co-Authors Dwi Aris Agung Nugrahaningsih Eti Nurwening Sholikhah Fiqri, Hairil Herzan Marjawan Mubarika Haryana, Sofia Nilasari, Fita Pratiwi, Woro Rukmi Rocca, Matteo Sofia Mubarika Haryana Temartenan, Jecklyn Shindy Zafrullah, Umar Farooq