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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Biotechnology Biology, Medicine, & Natural Product Chemistry Majalah Obat Tradisional The Indonesian Biomedical Journal Majalah Farmaseutik Indonesian Journal of Chemistry Indonesian Contemporary Nursing Journal (ICON Journal) Indonesian Journal of Cardiology Journal of Community Empowerment for Health Devotion: Journal of Research and Community Service The Avicenna Medical Journal Indonesian Journal of Pharmacology and Therapy
Dwi Aris Agung Nugrahaningsih
*) Department Of Pharmacology And Therapy, Faculty Of Medicine, Public Health And Nursing, Universitas Gadjah Mada; *) Herbal Medical Center, Faculty Of Medicine, Public Health, And Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Insecticide resistance and mechanisms of aedes aegypti (Diptera: Culicidae) in Yogyakarta, Indonesia Budi Mulyaningsih; Sitti Rahmah Umniyati; Tri Baskoro Tunggul Satoto; Ajib Diptyanusa; Dwi Aris Agung Nugrahaningsih; Yahiddin Selian
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 1 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.745 KB) | DOI: 10.19106/JMedSci005001201803

Abstract

For several decades, applications of organophosphates and pyrethroids insecticides have been extensively used to control Aedes aegypti as the primary dengue vector. Hence it is important to study dengue vector resistance status and its mechanisms in relation to long term use of insecticides. This study aimed to determine the resistance status and to characterize mechanisms of Ae. aegypti to organophosphates and pyrethroids using biological, biochemical and molecular assays. Larvae and puppae of Ae. aegypti were collected in the field of Plosokuning, Minomartani, Sleman, Yogyakarta, Indonesia. The biological assay was carried out using CDC Bottle Bioassay to test the resistant status to malation and cypermetrin. The biochemical assay was conducted using microplate assay with substrate α-naphthyl acetate to test the presence of esterase elevated activity, and the molecular assay was done using PCR with AaSCF7 and AaSCR7 primer to detect of point mutation at 1534 site, that located in the area of segment 6 of domain III. The biological assay showed Ae. aegypti suggests the possibility of resistance to malathion (mortality 82%) that needs to be confirmed further and already resistant to cypermetrin (mortality 76%). The biochemical assay of Ae. aegypti showed the presence of non-specific esterase elevated activity. The PCR method showed specific DNA bands were formed with the size of 748bp, and with sequencing showed there has been F1354C point mutation of voltage gated sodium chanel gene in the area of segment 6 of domain III. Long term use of insecticides did not successfully eliminate the targeted dengue vector, because Ae. aegypti mosquitoes were resistant to both insecticides. The results demonstrate the importance of designing better health policies regarding insecticide usage
Chloroquine and hydroxychloroquine for COVID-19 treatment Dwi Aris Agung Nugrahaningsih; Eko Purnomo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 52, No 3 (2020): Special Issue: COVID-19
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.349 KB) | DOI: 10.19106/JMedSciSI005203202002

Abstract

Coronavirus disease 2019 (COVID-19) is an emerging disease caused bysevere acute respiratory syndrome coronavirus-2 (SARS-CoV-2) that has been causing many people around the world affected. There is no approved treatment for COVID-19. Meanwhile, vaccine development still needs a long time before it becomes available to protect people from contracting COVID-19. Repurposing the available drugs is one of the fastest ways to get COVID-19 treatment. Studies have been conducted to discover for COVID-19 treatment that results in the finding of potential medication for COVID-19. Chloroquine and hydroxychloroquine are some of the available medication that shows potential for COVID-19 treatment. Preclinical study showed that the both drugs are active against SARS-CoV-2 in vitro. A pilot clinical study also showed their efficacy in COVID-19 treatment. Many clinical trials are now being conducted to prove their safety and efficacy for the prevention and treatment of COVID-19. However, until now there are not enough data to support the use of these drugs in COVID-19 management. Under the pressure to treat COVID-19 patients with chloroquine or hydroxychloroquine, clinicians shouldnot use these drugs for COVID-19 without considering the available information regarding theiruse for COVID-19. This review summarized the evidence regarding the potential of chloroquine and hydroxychloroquine in COVID-19 management.
Effects of Poly-herbal Tablet as Herbal Medicine on Lipid Level Woro Rukmi Pratiwi; Eti Nurwening Sholikhah; Dwi Aris Agung Nugrahaningsih; Mia Munawaroh Yuniyanti; Mustofa Mustofa; Ngatidjan Ngatidjan
Majalah Obat Tradisional Vol 24, No 1 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.299 KB) | DOI: 10.22146/mot.43108

Abstract

Dyslipidemia is one of the high-risk factors that cause cardiovascular disease and the prevalence is increasing all the time. Garlic (Alii sativi) has been suggested to reduce serum lipid level. Poly-herbal is herbal medicine that has been widely used in Indonesia, and it contains Alii sativi, Belericae fructus, Curcumae aeroginosae, and Amomi fructus extract. This study aimed to evaluate the effects of poly-herbal tablet on lipid levels in dylipidemia subjects. The study was conducted for 6 weeks on 58 dyslipidemic subjects. The subject was divided into 2 groups. Group 1 was given Poly-herbal with the dose 2x1200 mg, and group 2 was not given anti-dyslipidemic drugs (control group). Serum lipid level (total cholesterol, LDL, HDL, and triglycerides) were checked before the treatment (H0) and after the treatment (M6). There was a significant difference on the reduction of serum lipids level between the group that given Poly-herbal vs control group, respectively total cholesterol (-12.04±3.2 vs 8.38±4.3; p=.000),  LDL (-4.42±2.8 vs 6.93±4.7; p=.049) and HDL (-2.62+1.0 vs 3.31+1.4; p=.005). Triglyceride level on Poly-herbal group is not significantly decreased. In Poly-herbal group there was also a decrease in HDL levels which was not statistically significant (-2.62±1, p=.015). This is probably due to the HDL baseline in the control group included in the normal HDL category (57.38 ± 14.9) so that the HDL levels after being given treatment did not increase. This study suggests that using Poly-herbal in dyslipidemia subjects can reduce the level of total cholesterol and LDL.
Effects of Polyherbal Tablet for Hypertensive Patients Woro Rukmi Pratiwi; Eti Nurwening Sholikhah; Dwi Aris Agung Nugrahaningsih; Mia Munawaroh Yuniyanti; Mustofa Mustofa; Setyo Purwono
Majalah Obat Tradisional Vol 25, No 3 (2020)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.62363

Abstract

Hypertension is a degenerative disease whose prevalence is high and continues to increase over time. Polyherbal tablet have long been used by Indonesian people for hypertension drugs containing garlic (Alium sativum), jelawe (Belericae fructus), temu ireng rhizomes (Curcumae aeruginosae) and kapulaga (Amomi fructus) extracts. This study aims to determine the effect of polyherbal tablet on hypertensive patients. The subjects of 29 hypertensive patients participated in a open-randomized-without comparison-study for 6 weeks. Subjects were divided into 3 groups, each subject received polyherbal tablet with a dose of 2x600 mg (group 1), 2x1200 mg (group 2) and 2x2400 mg (group 3). Blood pressure was monitored on D-0, D-3, D-5, D-7, W-2, W-3, W-4, W-5 and W-6. There was a significant decrease in systolic and diastolic blood pressure at the sixth week in all three treatment groups. The highest decrease in systolic blood pressure was in group 1 while diastolic blood pressure was in group 2 (-22.94 ± 5.871 and 13.13 ± 4.324 respectively). The lowest reduction in systolic and diastolic blood pressure occurred in the third group (-15.07 ± 3,519 and 8.98 ± 3,394 respectively). This study suggests that polyherbal tablet contains garlic (Alii sativi), jelawe (Belericae Fructus), temu ireng rhizome (Curcumae aeruginosae) and cardamom (Amomi Fructus) can reduce blood pressure in patients with hypertension. 
Phytochemical Screening and Antidengue Activity of Jatropha Multifida Extract against DENV-2 Mia Munawaroh Yuniyanti; Dwi Aris Agung Nugrahaningsih; Mae Sri Hartati Wahyuningsih
Majalah Obat Tradisional Vol 27, No 1 (2022)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.73368

Abstract

Cases of dengue infection are still high in various parts of the world and no antiviral has been found to treat dengue infection. Jatropha multifida is one of the herbs used by the community to treat dengue infection, but pre-clinical and clinical scientific evidence has not been carried out. Phytochemical screening of ethanol extract of Jatropha multifida leaves was carried out using thin layer chromatography. Cytotoxic assay was performed on Vero cells using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide). The cytotoxic concentration 50 (CC50) was determined using probit analysis. Anti-dengue test on dengue  virus serotype 2 (DENV-2) was performed on Vero cells and the RNA (ribonucleic acid) copy number was quantified using quantitative polymerase chain reactions ((qPCR). Phytochemical screening results showed that the ethanol extract of Jatropha multifida leaves contains flavonoid and terpenoid compounds. CC50 of the extract was found to be 651.8 µg/mL. The RNA copy number of the treated group was lower than the control group and the difference was significant. The ethanol extract of Jatropha multifida leaves has anti-dengue activity against DENV-2.
Detection of Polymorphism on Voltage-gated Sodium Channel Gene of Indonesian Aedes aegypti Associated with Resistance to Pyrethroids Budi Mulyaningsih; Sitti Rahmah Umniyati; Tri Baskoro Tunggul Satoto; Ernaningsih Ernaningsih; Dwi Aris Agung Nugrahaningsih
The Indonesian Biomedical Journal Vol 10, No 3 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i3.425

Abstract

BACKGROUND: Aedes aegypti is a vector of several pathogens including dengue virus. Vector control is an effective way to break the transmission but unfortunately constant use of insecticides ultimately causes vector resistance. Pyrethroids have been used for about 15 years to combat Ae. aegypti in Yogyakarta Province, Indonesia. Single amino acid substitutions in the voltagegated sodium channel associated with pyrethroid resistance constitute one of the main causative factors of knockdown resistance (kdr). The aim of this study is to detect resistant status to cypermetrine and polymorphism on the voltagegated sodium channel gene of Ae. aegypti from 2 dengue endemic areas in Yogyakarta Province (Yogyakarta city and Sleman district).METHODS: Pyrethroid resistance in Ae. aegypti mosquitoes was detected by using CDC Bottle Bioassay. To detect the polymorphism on the voltage-gated sodium channel gene of Ae. aegypti analyses were conducted by using PCR and direct DNA squencing with primers AaSCF1 and AaSCR4 for S989P, I1011M (or V), L1014F sites, and AaSCF7 and AaSCR7 for the F1534C site.RESULTS: According to bioassay, the results for Ae. aegypti from Yogyakarta city (93% mortality) and Sleman district (88% mortality) suggest the possibility of resistance to cypermethrin. We observed polymorphism on voltagegated sodium channel gene on site F1534C (heterozygous).CONCLUSION: The findings provide early evidence that the use of cypermethrin (pyrethroids) in Yogyakarta city and Sleman district, Yogyakarta Province, Indonesia is reducing its effectiveness to control Ae. aegypti. Recommendations include additional tests for confirmation.KEYWORDS: Aedes aegypti, dengue virus, cypermethrin, Bioassay, Yogyakarta
BMPR2 Editing in Fibroblast NIH3T3 using CRISPR/Cas9 Affecting BMPR2 mRNA Expression and Proliferation Dwi Aris Agung Nugrahaningsih; Eko Purnomo; Widya Wasityastuti; Ronny Martien; Nur Arfian; Tety Hartatik
The Indonesian Biomedical Journal Vol 14, No 1 (2022)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v14i1.1724

Abstract

BACKGROUND: Bone Morphogenetic Protein Receptor II (BMPR2) deficiency is associated with the pathologic development of pulmonary vascular changes in Pulmonary Arterial Hypertension (PAH). Fibroblast is the most abundant cell in vascular. However, there is only a little information regarding the effect of BMPR2 deficiency in fibroblast. This study aims to understand the effect of BMPR2 deficiency in fibroblasts.METHODS: This study applied the CRISPR/Cas9 technique to edit BMPPR2 in NIH-3T3 cells. The transfection of CRISPR/Cas9 for BMPR2 editing into NIH-3T3 cells was done by using chitosan nanoparticles. The evaluation of BMPR2 and Transforming Growth Factor (TGF)-β mRNA expression was done using Quantitative real-time polymerase chain reaction. The assessment of edited NIH-3T3 cells proliferation was done using a scratch test assay.RESULTS: The BMPR2 mRNA expression of CRISPR/Cas9-edited group was lower than the untreated group. The proliferation of the CRISPR/Cas9-edited group was higher than the untreated group. The TGF-β mRNA expression of CRISPR/Cas9-edited and untreated groups was similar.CONCLUSION: BMPR2 deficiency in fibroblast increase the fibroblast ability to proliferate.KEYWORDS: BMPR2, PAH, fibroblast NIH-3T3, CRISPR/Cas9, proliferation 
The effect of chronic usage of Renin-Angiotensin-Aldosterone System Blocking Agents on Incidence of Contrast-Induced Nephropathy in patients with Diabetes Mellitus and Renal Insufficiency. A Restropesctive Cohort Study astika widy utomo; dwi aris agung nugrahaningsih; iwan dwiprahasto
Jurnal Kardiologi Indonesia Vol 40 No 1 (2019): Indonesian Journal of Cardiology: January-March 2019
Publisher : The Indonesian Heart Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30701/ijc.v40i1.920

Abstract

Background: This study was to investigate the effect of long term use of Renin-Angiotensin-Aldosterone System(RAAS) blocking agents on the incidence of Contrast-Induced Nephropathy(CIN) on patients with Diabetes Mellitus(DM) and renal insufficiency underwent Percutaneous Coronary Intervention (PCI). Methods:A total 281 of subjects were included in this study and divided into two groups based on prior use of RAAS blocking agents (RAAS +, n = 146; RAAS -, n = 135). CIN was defined as an increase of ≥25% in creatinin over baseline value 48-72 hours after PCI. Result: Total incidence of CIN was 14,95%. There was no difference in the incidence of CIN between 2 study groups (p = 0,952) and relatif risk for CIN was 1,02. Left Ventricular ejection Fraction (LVEF) ≤ 40 % (OR 2,300; 95% CI 1,028 – 5,143; p = 0,043), anemia (OR 2,628; 95% CI 1,274 – 5,422; p = 0,009) and Glomerular Filtration rate (GFR) pre PCI ≤ 60 mL/menit (OR 2,782; 95% CI 1,293 – 5,987; p = 0,009) were important predictors of CIN. Conclusion: Long term use of RAAS blocking agents do not increase the incidence of CIN on patients with DM and renal insufficiency underwent PCI.
Optimasi Carbomer, Propilen Glikol, dan Trietanolamin Dalam Formulasi Sediaan Gel Ekstrak Etanol Daun Kembang Bulan (Tithonia diversifolia) Amira Fawwaz Tsabitah; Abdul Karim Zulkarnain; Mae Sri Hartanti Wahyuningsih; Dwi Aris Agung Nugrahaningsih
Majalah Farmaseutik Vol 16, No 2 (2020)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v16i2.45666

Abstract

Ekstrak etanol daun kembang bulan atau Tithonia diversifolia telah diteliti memiliki kemampuan sebagai anti keloid yang baik. Tujuan penelitian ini adalah untuk mendapatkan formula gel dengan sifat fisik dan stabilitas yang baik. Metode yang digunakan dalam optimasi ini adalah Simplex Lattice Design. Formula optimum gel dibuat dari bahan carbomer, propilen glikol, dan trietanolamin. Parameter yang diukur adalah viskositas, pH, daya sebar, freeze and thaw cycles, dan sineresis. Hasil uji kemudian dianalisis menggunakan paired t-test.Hasil penelitian menunjukan bahwa formula optimum gel terdiri dari 0,81% carbomer, 5,9% propilen glikol, dan 0,58% trietanolamin dengan viskositas 155,96 d.Pas, daya sebar 4,06 cm, pH 5,50, dan sineresis 0,64%. Basis gel yang dihasilkan bening, memiliki viskositas 156,10 ± 2,87 d.Pas, daya sebar 3,95 ± 0,07 cm, pH 5,50 ± 0,00, dan sineresis 0,46 ± 0,00 %. Sediaan basis gel stabil dalam uji stabilitas fisik. Gel ekstrak etanol daun kembang bulan yang dihasilkan memiliki viskositas 77,70 ± 2,30 d.Pas, daya sebar 4,55 ± 0,21 cm, pH 5,32 ± 0,01, dan sineresis 1,42 ± 0,28 %. Sediaan gel ekstrak etanol daun kembang bulan kurang stabil dalam uji stabilitas fisik pada viskositas yang mengalami penurunan, daya sebar yang mengalami kenaikan, pH yang mengalami kenaikan, dan sineresis yang meningkat.
Increasing knowledge about nutrition and health in the elderly and cadres in Banguntapan Village, Bantul Mae Sri Hartati Wahyuningsih; Dwi Aris Agung Nugrahaningsih; Probosuseno Probosuseno
Journal of Community Empowerment for Health Vol 3, No 1 (2020)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jcoemph.44880

Abstract

Increase the number of elderly is one of the social problems that require the attention of all involved parties. Health care efforts are now aimed at keeping the elderly healthy, active, independent, and productive socially and economically for themselves, their families and the community. Elderly is an age group where a person has experienced various decreases in body functions that can affect appetite, which ultimately leads to malnutrition. We designed community service-based study to increase the elderly health in the Banguntapan Village through increasing the awareness of the elderly about managing their health, the knowledge and skill to take care elderly of the families who have elderly on their care in Banguntapan Village. The method applied in this activity is counseling with pre-test and the first post-test and distribution pocket of about nutrition and elderly health. To be able to measure the retained knowledge after the activities, we also carried out the second post-test four weeks after the first meeting. The data were analyzed by descriptive analysis, and a paired t-test was performed to test the pre-test and post-test data. The total participants who participated in this activity were 144 people consisting of all cadres in Banguntapan Village, plus the elderly and families who have elderly in Karangbendo Hamlet. This research showed that there was an increase in knowledge about health and nutrition between the 1st pretest-posttest; between the 1st post-test and 2nd post-test; and between the second pretest and posttest (11.14; 14.86; 28.03)%. The counseling, discussion, and distribution of pocketbooks can increase the knowledge of the elderly, family and volunteer in Banguntapan and Karang Bendo Village.
Co-Authors Abdul Karim Zulkarnain Ade Saputri Aditya Nugraha Ahmad Hamim Sadewa Ajib Diptyanusa Akhmad Kharis Nugroho Amira Fawwaz Tsabitah Andina Setyawati Andrew Limavanady Ani Kristiyani Ari Wibowo Astika Widy Utomo, Astika Widy Atmaja, Sarah Aurelia Priscilla Regita Putri Budi Mulyaningsih Budi Mulyaningsih Buftheim, Sonia Caroline Caroline Christantie Effendy Christine Patramurti Denny Agustiningsih Dimas Adhi Pradana Dwi Liliek Kusindarta Eko Purnomo Ellsya Angeline Rawar Emilia Vivi Arsita Erna Kristin Ernaningsih Ernaningsih Eti Nurwening Sholikhah Eti Nurwening Sholikhah Foe, Kuncoro Gloria Evita Thalia Hendy Wijaya Hera Nirwati Herzan Marjawan Hevi Wihadmadyatami I Made Andika Bara Kusuma Ikhsan Nur Salim Ismawati, Yuyun Iwan Dwiprahasto Januar Rizqi Jonathan J Jumina Jusak Nugraha Katharina Oginawati Ketut Shri Satya Wiwekananda Mae Sri Hartati Wahyuningsih Mahatma Sotya Bawono Mia Munawarroh Yuniyanti Mosa Rini Nurul Hidayati Mubarika Haryana, Sofia Mustofa Mustofa Mustofa Mustofa Mustofa Mustofa Ngatidjan Ngatidjan Nico Jafet Nilasari, Fita Nur Arfian Oryza Chrisantia Pamungkas Bagus Satriyo Prasetyastuti Prasetyastuti Pratiwi, Woro Rukmi Probosuseno Probosuseno Raden Sumiharto Rahman, Intan Nur’azizah Rahmi Ayu Wijayaningsih Rahmi Ayu Wijayaningsih Rasya Mayora Ratna Sari Rizki Awaluddin Rocca, Matteo Ronny Martien Rul Afiyah Syarif Sam Ibeneme Satriyo, Pamungkas Bagus Senny Yesery Esar Setyawan Bekti Wibowo Setyo Purwono Siswanto Siswanto Sitti Rahmah Umniyati Sitti Rahmah Umniyati Sofia Mubarika Haryana Srikanth Karnati Süleyman Ergün Tety Hartatik Titien Budhiaty Tri Baskoro Tunggul Satoto Widya Wasityastuti Wuryanto Hadinugroho Yahiddin Selian Yasmeen Kwok Yudy Tjahjono Yufita Ratnasari Wilianto Yuliani, Fara Silvia Yuniyanti, Mia Munawaroh Zafrullah, Umar Farooq