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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology BERITA BIOLOGI
Sri Widawati
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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PHYTASE PRODUCTION BY Enterobacter cloacae Suliasih Suliasih; Sri Widawati
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.0.0.0.1233

Abstract

 This study aims to isolate the phytase producing bacteria (PPB), a plant growth promoting rhizobacteria (PGPR), from 1Cigna sinensis rhizosphere and to optimize its physicochemical conditioning. Phytase is an enzyme that can hydrolyze the phosphoester bond in organic phosphorus (phytic acid) to form ester phosphate and inorganic phosphate, the available forms of phosphorus. To test its ability to hydrolyze organic phosphates (calcium phytate), the phytase was screened in solid and liquid phytase screening medium (PSM). After isolation, a total of 13 bacteria were positive for this enzyme’s production as indicated by the clear zones of hydrolysis observed around the colony. Enterobacter cloacae strain B1 had the largest hydrolysis efficient (3.43) on solid medium. The phytase-production of the Enterobacter cloacae strain grown in liquid PSM, showed 0.92 U/mL after 48 hours of incubation. This strain produced optimum levels of phytase in the presence of lactose and monoammonium phosphate (NH4H2PO4), as carbon and nitrogen sources, respectively, at 30 °C and pH 5.0. The PPB obtained in this study are recommended for further research as to their use as plant biological fertilizers.  
THE POPULATION OF NITROGEN FIXING BACTERIA AND PHOSPHATE SOLUBILIZING BACTERIA IN THE RHIZOSPHERE FROM GUNUNG HALIMUN NATIONAL PARK Sri Widawati; Suliasih Suliasih
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i6.1075

Abstract

The biodiversity of nitrogen fixing bacteria (Rhizobium, Azotobacter and Azospirillum) and phosphate solubilizing bacteria in rhizosphere collected from Gunung Halimun National Park were studied.Bacterial population was counted from soil rhizosphere collected from various ecosystem types (determined based on elevation) namely 600 m, 1000 m, 1100 m, 1500 m and 1800 m above sea level.The results showed that the highest population of N-fixing and P solubilizing bacteria at Cikaniki (1100 asl) are founded in the plant rhizosphere of Schima wallichii in plot Suzuki A I and Altingia exelsa in Suzuki A III,at Gunung Botol area is in 1000 m asl,and Ciptarasa area is in 600 m asl.The population of N-fixing and P solubilizing bacteria at Gunung Halimun was influenced by the vegetation type, soil pH,and the elevation of area. Rhizobium, Azotobacter, Azospirillum (N-fixing bacteria); Pseudomonas sp, and Bacillus megaterium (P solubilizing bacteria), are most dominant at 600 m, 1000 m and 1100 m asl.
Co-Authors Suliasih Suliasih Suliasih Suliasih