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All Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Asian Journal of Health and Applied Sciences
Lutfi, Nadira Nur Hajah
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences

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Analysis of The Heavy Metal Content of Zinc (Zn) In Tofu Using Atomic Absorption Spectrophotometer (AAS) Lutfi, Nadira Nur Hajah; Rizal, Fahmi
Asian Journal of Health and Applied Sciences Vol. 1 No. 3 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53402/ajhas.v1i3.191

Abstract

Tofu, which is rich in protein, has long been consumed by the people of Indonesia as a side dish. Tofu is a food made from fermented soybeans and extracts from it. One of the metals that is dangerous and should not be eaten is zinc (Zn). Zinc is a type of heavy metal that is soft and easy to purify. This study aims to determine the content of heavy metal zinc (Zn) contained in tofu by using Atomic Absorption Spectrophotometry (AAS). The advantages of an Atomic Absorption Spectrophotometer (AAS) are specific, low detection limit, from the same solution, several different elements can be measured, can be applied to many types of elements in many types of samples, limit levels that can be determined are very area (mg/L to percent). This research uses a descriptive-analytical method to describe or provide an overview of the object under study through the data. And this research uses the wet destruction method, namely the destruction of the sample with the addition of a solution of nitric acid (HNO3) followed by heating and filtering. The results showed that the concentration of heavy metal Zn in tofu was 0.161 ppm, 0.139 ppm, 0.161 ppm, 0.152 ppm, 0.153 ppm, 0.154 ppm, 0.159 ppm, 0.174 ppm, 0.185 ppm, 0.141 ppm, 0.159 ppm and 0.164 ppm. The conclusion is that the levels of tofu Zn contain zinc metal Zn with levels of 10.3325 mg/kg. The levels of zinc metal Zn are still below the threshold level, so tofu is still safe for consumption.
Chemometric Analysis of Serum Magnesium Calculations Using Mg-Xylidyl Blue-I Method Based on Molar Absorptivity Kafesa, Ally; Lutfi, Nadira Nur Hajah; Wahyu, Cep
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.1876

Abstract

The concentration of magnesium is determined based on the absorbance of the Mg-Xylydil Blue-I complex solution use spectrophotometer. Based on the Lambert-Beer rule, the calculation of sample concentration is based on the formula A = Ԑ. b. C. Generally, the thickness of the cuvette (b) and the molar absorptivity (Ԑ) factor will be ignored because it is considered to have a fixed value, therefore the sample concentration is measured based on the ratio of the absorbance of the sample against the standard solution. However, the standard solution contains pure magnesium and has a different matrix than the sample matrix, so this condition can give analytical errors and lead to misinterpretation of the results. The purpose of this study was to determine the accuracy and the precision of serum magnesium calculation by the principle of the Mg- Xylydil Blue-I complex reaction based on molar absorptivity compared to the general method. This research uses comparative study design methods. The serum sample used was the patient's serum specimen who has a normal magnesium level. The results showed that the significance value of the paired t-test statistical was 0.000 (p < 0.05). The accuracy value (d%) of the calculation formula uses Ɛ is 0.00 and the precision value (CV%) is 0.53. While the accuracy value (d%) of the calculation formula without Ɛ is 0.00 and the precision value (CV%) is 0.38. Calculations based on molar absorptivity (Ɛ) can measure more significant serum magnesium than those calculated based on standard magnesium solutions.
Co-Authors Fahmi Rizal Kafesa, Ally Wahyu, Cep