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All Journal EduChemia: Jurnal Kimia dan Pendidikan Chimica et Natura Acta Indonesian Journal of Chemistry Molekul: Jurnal Ilmiah Kimia Bulletin of Chemical Reaction Engineering & Catalysis

Mulyani, Irma

Institut Teknologi Bandung

Author-ID : 1792762

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Materials Science & Nanotechnology

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Articles

Metode Non-Enzimatik Riboflavin-Nitrobluetetrazolium (Rb-NBT) Sebagai Teknik Penentuan Aktivitas Penangkal Radikal Bebas Anion Superoksida pada Kompleks Mangan(III)-Salen Yusi Deawati; Djulia Onggo; Irma Mulyani; Yuli Ainun Rosidah; Iwan Hastiawan; Ratna Annisa Utami; Dikdik Kurnia
Chimica et Natura Acta Vol 6, No 1 (2018)
Publisher : Departemen Kimia

Pada penelitian ini dilakukan pengujian aktivitas penangkal radikal bebas superoksida secara in vitro terhadap senyawa kompleks polimerik [Mn(salen)N(CN)2]n, baik dengan metode non-enzimatik maupun enzimatik. Aktivitas penangkal radikal bebas superoksida yang didapatkan dari kedua metode ini dinyatakan dengan IC50. Melalui metode non-enzimatik riboflavin-nitrobluetetrazolium (Rb-NBT), radikal superoksida yang dihasilkan dari penyinaran riboflavin, bereaksi dengan nitrobluetetrazolium sebagai indikator dan senyawa kompleks, lalu serapan NBT tereduksi diukur pada panjang gelombang 560 nm. Dengan metode non-enzimatik ini, dihasilkan nilai IC50 yang tinggi, konsisten, dan standar deviasinya sangat kecil, yaitu 3,4 ± 0,4 µM. Sedangkan secara enzimatik, radikal superoksida yang dihasilkan dari reaksi antara xantin dan xantin oksidase ditangkap oleh sitokrom C (sebagai indikator) dan senyawa kompleks, kemudian sitokrom C tereduksi diukur serapannya pada panjang gelombang 450 nm. Melalui metode enzimatik McCord-Fridovich (McCF) ini, dihasilkan beberapa nilai IC50 kompleks [Mn(salen)N(CN)2]n yang tidak konsisten, yang disebabkan oleh tidak berfungsinya enzim. Dengan demikian, metode non-enzimatik Rb-NBT lebih mudah dan lebih akurat untuk digunakan sebagai teknik penentuan aktivitas penangkal radikal anion superoksida dibandingkan dengan metode enzimatik.

Synthesis, Characterization and Antioxidant Activity of Kobalt (II)-Hydrazone Complex Yulyani Nur Azizah; Irma Mulyani; Deana Wahyuningrum; Damar Nurwahyu Bima
EduChemia (Jurnal Kimia dan Pendidikan) Vol 5, No 2 (2020)
Publisher : Department of Chemical Education Faculty of Teacher Training and Education Universitas Su

The potency of the first-row transition metal complexes with Schiff's base ligand as an antioxidant has been widely studied by researchers. In this study, hydrazone-type Schiff base compounds, salicylaldehyde-2,4-dinitrophenylhydrazone (SDPH), resulted from the condensation of salicylaldehyde (1.5 mmol) and 2,4-dinitrophenylhydrazine (1.5 mmol) were synthesized and characterized with 1H-NMR. The synthesized SDPH was used as ligands in the formation of Co(II) metal complexes. The Co(II)-SDPH complexes were synthesized with reflux reaction at 60oC for 6 hours in molar ratio 1:3. Triethylamine is used to synthesis Co(II)-SDPH, which serves to deprotonate SDPH ligands. ESI-MS analysis showed that the complex is a neutral dimeric complex.The complex has the chemical formula [Co2(SDPH)2[(Et)3N]2(acn)2(OH)2} ([M+Na]+ = 1061 g/ mol), shown on the ESI-MS spectrum. Coordinate bonds between Co (II) metal ions with ligands are observed in the presence of shifting of the C=N wave number, as well as the loss of the vibrating peaks of the O-H groups to the infrared spectra of complexes. The IR spectrum of complexes was suggested that SDPH behave as a bidentate ligand through the azomethine nitrogen atom and phenolic oxygen atom. The ligand and its Co(II) complex have been screened for antioxidant activity against DPPH (2,2-diphenyl-1-picrylhydrazine). Based on the activity test results, the IC50 value of Co(II)-SDPH complex was lower than ligand. These results show that Co(II)-SDPH are potentials as antioxidants.

Activity of Superoxide Dismutase Mimic of [Mn(salen)OAc] Complex Compound Non-enzymatically in Vitro Through Riboflavin Photoreduction Yusi Deawati; Djulia Onggo; Irma Mulyani; Iwan Hastiawan; Dikdik Kurnia
Molekul Vol 12, No 1 (2017)
Publisher : Universitas Jenderal Soedirman

The complex compound of [Mn(salen)OAc] can serve as mSOD and its activity has been determined non-enzymatically in vitro through riboflavin photoreduction. The complex was synthesized from Mn(OAc)2.4H2O and H2salen. Based on the elemental analysis, the C=56.69%; H=4.21%; and N=7.52% content are corresponding to the chemical formula of MnC18H17N2O4. The functional groups and ionic species in the complex have been analyzed by infrared spectroscopy and ESI-MS. SOD activity was determined by mixing complex at various concentrations with riboflavin and nitroblue tetrazolium (NBT), then the mixture was lighted with 20 Watt tungsten lamp for 15 minutes in a closed box. Then the reduced NBT absorptions were measured at Î» 560 nm. The difference of absorbance between standard and sample solutions (without and with riboflavin, respectively) was multiplied by 100% to obtain %inhibition of each various sample concentration against NBT. SOD activity was obtained from IC50 data defined as a 50% inhibition of the plot curve of % inhibition to the concentration of the complex. The result obtained for this compound is IC50 = 2.7 Â± 0.05 ÂµM as well as enzymatic method. Therefore, this method can be used to determine the SOD activity by giving more stability and accuracy of IC50 value.

Synthesis, Characterization, and Magnetic Properties of Iron(II) Complex with 2,6-Bis(pyrazol-3-yl)pyridine Ligand and Tetracyanonickelate Anion Fitriani Fitriani; Irma Mulyani; Djulia Onggo; Kristian Handoyo Sugiyarto; Ashis Bhattacharjee; Hiroki Akutsu; Anas Santria
Indonesian Journal of Chemistry Vol 23, No 4 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.81625

The complex containing iron(II), 2,6-bis(pyrazol-3-yl)pyridine (3-bpp) as ligand, and tetracyanonickelate as counter anion has been synthesized and characterized. The characterization data suggest the corresponding formula of [Fe(3-bpp)2][Ni(CN)4]·4H2O. Meanwhile, the SEM–EDX analysis image confirms the existence of all elements contained in the complex except the hydrogen atom. The infrared spectra exhibit vibration bands of the functional groups of 3-bpp ligand and [Ni(CN)4]−1 anion. From magnetic property measurement, the complex's molar magnetic susceptibility (XMT) value is 2.65 emu mol−1 K at 300 K, which contains about 75% high-spin state of the Fe(II) complex. Upon lowering the temperature, the XMT value gradually decreases around 1.37 emu mol−1 K at 13 K. It decreases sharply to about 0.73 emu mol−1 K at 2 K. These values reveal that Fe(II) complex is in the low-spin (LS) state. As a result, the complex exhibited spin-crossover characteristics of gradual transition without thermal hysteresis, and the transition temperature occurred below room temperature with a transition temperature (T1/2) close to 140 K. The spin crossover property of the complex is supported by a thermochromic reversible color change from red-brown at room temperature to dark brown on cooling in liquid nitrogen associated with the high-spin to low-spin transition.

Investigation of Chlorophyl-a Derived Compounds as Photosensitizer for Photodynamic Inactivation Listiana Oktavia; Irma Mulyani; Veinardi Suendo
Bulletin of Chemical Reaction Engineering & Catalysis 2021: BCREC Volume 16 Issue 1 Year 2021 (March 2021)
Publisher : Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.9767/bcrec.16.1.10314.161-169

Chlorophyll has unique physicochemical properties which makes them good as photosensitizer of Photodynamic Inactivation (PDI). The physicochemical properties of chlorophyll as photosensitizer can be optimized through several routes. One of the possible route is by replacing the metal ion center of chlorophyll with other ions. In this research, the effect of coordinated metal ion in the natural chlorophyll-a was studied for bacterial growth (S. aureus) inhibition. The replacement of metal in the center of chlorophyll hopefully can improve the intensity of Intersystem Crossing Mechanism (ISC) lead to the formation of singlet oxygen species. The chlorophyll a and b were isolated from spinach via precipitation technique using 1,4 dioxane and water. The chlorophyll a and b were separated using sucrose column chromatography. The thin layer chromatography result showed that chlorophyll a (Rf: 0.57) had been well separated with chlorophyll b (Rf: 0.408). The absorption spectra of chlorophyll a and b showed that the Soret band was observed at 411 and 425 nm, while the Q band appeared at 663 and 659 nm. Replacement of metal ion center shifted the Soret band of chlorophyll- a derivatives to lower energy region, while Q-band was slightly shifted to the higher energy region. The absorption and the fluorescence intensity were also observed decreasing after ion replacement. The Inhibition activity investigation over S. aureus showed the highest inhibition activity was exhibited by Zn-pheophytin-a (66.8%) followed by chlorophyll a (30.1 %) and Cu-pheophytin-a (0%). The inhibition activity is correlated with decreasing fluorescence intensity. The formation of singlet oxygen by ISC mechanism is hypothesized to deactivate the excitation state of Cu-pheophytin-a. Copyright © 2021 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).

Co-Authors Anas Santria Ashis Bhattacharjee Damar Nurwahyu Bima Deana Wahyuningrum Dikdik Kurnia Djulia Onggo Djulia Onggo Djulia Onggo Fitriani Fitriani Hiroki Akutsu Iwan Hastiawan Iwan Hastiawan Kristian Handoyo Sugiyarto Listiana Oktavia Ratna Annisa Utami Veinardi Suendo Yuli Ainun Rosidah Yulyani Nur Azizah Yusi Deawati Yusi Deawati

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