Corona virus disease 2019 is still developing with various new variants. Protection of health workers from the risk of infection is very important. Preventing the transmission of Covid-19 to health workers while carrying out their duties starts from themselves through a good work culture, self-efficacy and commitment to health protocols that have been established by the government. The research objective is to identify the relationship between self-efficacy and self-intention of health workers in sustainable prevention of Covid-19 in NTT. The quantitative research method uses a cross sectional study design, the population of health center health workers is 2233 people with a sample of 300 people. The research results showed that there was an influence of work culture on Covid 19 prevention behavior (p=0.001). Self-efficacy influences Covid-19 prevention behavior and self-intention influences Covid-19 prevention behavior in health workers. The conclusion is that work culture, self-efficacy and self-intention (intention) influence the Covid 19 prevention behavior of health workers. Suggestions for variables that influence Covid 19 prevention behavior to receive attention from the government