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All Journal Journal of Degraded and Mining Lands Management Bioma : Jurnal Biologi Indonesia
Zulkifliani, Zulkifliani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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SELEKSI SENYAWA PENGHIDROLISIS UNTUK MENGHASILKAN GULA REDUKSI DARI LIMBAH KULIT ARI KEDELAI SEBAGAI BAHAN FERMENTASI BIOETANOL Zulkifliani, Zulkifliani; Handayani, Siska; Adisyahputra, Adisyahputra; Sakarani, Devitra
Bioma Vol. 13 No. 1 (2017): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (657.44 KB) | DOI: 10.21009/Bioma13(1).1

Abstract

This study aims to determine the most effective compound used to hydrolyze soy husk waste to produce reducing sugar as raw material for bioethanol fermentation. The study was conducted at the Laboratory of Bioprocess PPPTMGB "LEMIGAS" in April-September 2015. The method used is experiment using a randomized block design consisting of two factors. The first factor is the type of compounds used in the process of hydrolysis, namely H2SO4, HCl, NaOH, and NH3. The second factor is the concentration of hydrolyze compound 0.2%, 0.4%. 0.6%, 0.8%, and 1% (v/v) and every treatment repeated 4 times. Parameters measured were content of reduced sugar hydrolysis product, and secondary data that content of cellulose and hemicellulose also the density of ethanol. Concentration of reducing sugar from hydrolysis of soybean husk is analyzed by two-way ANOVA test. ANOVA analysis result indicate that the best hydrolysis compounds in hydrolizing soybean husk is HCl with the optimum concentration is 0,4%. And there are interactions between treatment of compound used to hydrolyze as well as concentration on reducing sugar concentration (mg/mL) as product from soybean husk waste hydrolysis. Post-hoc test showed that HCl 0,4% produce the highest concentration of reducing sugar at 31.23 mg/mL.
Synergistic effect of Azotobacter sp. and bulking agents on hydrocarbon bioremediation performance in petroleum-contaminated soil Suryatmana, Pujawati; Hindersah, Reginawanti; Setiawati, Mieke Rochimi; Parnadi, Wahyudi Widyatmoko; Zulkifliani, Zulkifliani; Syafrizal, Syafrizal
Journal of Degraded and Mining Lands Management Vol. 13 No. 3 (2026)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2026.133.10369

Abstract

Petroleum hydrocarbon contamination is a critical environmental issue in tropical soils. This study evaluated the effects of Azotobacter sp. and two bulking agents: rice husk biochar (RHB) and spent oyster mushroom substrate (SOMS),  on a microcosm scale of petroleum-contaminated Ultisols. The experiment design used a randomized block design (RBD). Treatments included single and combined applications of Azotobacter sp. and bulking agents. Results showed that SOMS  amendment yielded significantly (p< 0.05) the highest microbial populations,  fungi (43.23 × 104 CFU g?¹) and bacteria (42.633 x 106 CFU g?¹), the highest degradation rate (0.040 mg L?¹ cell?¹ day?¹) and efficiency (81.62%) compared with the control (0.012 mg L?¹ cell?¹ day?¹; 39.55%). Azotobacter sp. inoculation combined with SOMS increased Azotobacter sp. density (59.167 × 104 CFU g?¹). Azotobacter sp. produced a biosurfactant that was dominated by Oleic acid. The hydrocarbon biodegradation rate improvements were linked to increased soil pH, nutrient availability, and biosurfactant production by Azotobacter sp. Meanwhile, RHB alone showed lower performance, but when combined with Azotobacter sp. improved degradation relative to the control. Integrating Azotobacter sp. inoculation with nutrient-rich bulking agents, particularly SOMS, significantly improves petroleum hydrocarbon biodegradation in Ultisols by optimizing physicochemical and biological conditions for indigenous microbial consortia.
Co-Authors Adisyahputra Adisyahputra, Adisyahputra Dwi Atmanto, Murachmad Masykur Lubad, Aziz Mieke Rochimi Setiawati Musu, Junita Trivianty Parnadi, Wahyudi Widyatmoko Pratama, Fami Adetyas Maulana Pujawati Suryatmana Reginawanti Hindersah Sakarani, Devitra Setijo Bismo Siska Handayani Sunarjanto, Djoko Syafrizal Syafrizal Usman Usman Yuliarita, Emi