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All Journal Biosaintifika: Journal of Biology & Biology Education Jurnal Ners
Latifah, Emi
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Agriculture, Biological Sciences & Forestry Health Professions Medicine & Pharmacology Nursing

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Lignolytic Enzyme Activity of Isolated Bacteria from Termite (Coptotermes Sp.) and Milkfish (Chanos chanos Forsskal, 1775) Guts Latifah, Emi; Mulyani, Putri Dwi; Purwestri, Yekti Asih
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.19333

Abstract

Bacteria BSR 2, Pseudomonas alcaligenes (BSR 3), Brevibacillus parabrevis (BSR 8), Brevibacillus sp. (BSR 9), isolated from termite gut and Bacillus licheniformis (BSA B1) isolated from milkfish gut have been known to possess celluloytic activity. However, their lignolytic ability has not been known. This study aimed to determine the lignolytic ability of bacteria isolated from termit (Coptotermes sp.) and milkfish (Chanos chanos Forsskal, 1775) guts and their enzymes characterization. The qualitative test was done through the spot test method, while quantitative assay was performed spectrophotometrically at 335 nm to calculate vanillin concentration. The isolates were grown in Lignin Mineral Medium, then the optical density (OD620) were measured every 24 hours for 5 days using spectrophotometer to determine their growth profile and the best isolation time of the lignolytic enzyme. Based on results, the best lignolytic enzyme isolation time for strains Bacillus licheniformis (BSA B1) and BSR 2 were 5 days, yielding lignolytic enzyme activity of 0.961 ± 0.168 U/mg and 2.176 ± 0.088 U/mg respectively,  while strains Pseudomonas alcaligenes (BSR 3), Brevibacillus parabrevis (BSR 8), and Brevibacillus sp. (BSR 9) were 4 days, yielding of 1.206 ± 0.045 U/mg, 1.162 ± 0.191 U/mg, and 0.896 ± 0.108 U/mg, respectively. The strain BSR 2 showed the highest lignolytic activity compared to other strains. The optimum temperature for lignolytic enzyme activity of BSR 2 was 30 ℃ and the optimum pH was 7. The lignolytic enzyme activity showed that these bacterial isolates can be a chance to be used as new alternative lignolytic enzyme source in commercial bioconversion process.
Hubungan Tuntutan Kerja dan Sumber Daya terhadap Stres Kerja di Sektor Pelayanan Kesehatan : Systematic Review Latifah, Emi; Shahuliyah, Zahroh; Purnami, Cahya Tri
Jurnal Ners Vol. 10 No. 2 (2026)
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v10i2.55988

Abstract

Background Healthcare workers are required to provide fast, accurate, and empathetic services, often under limited resources. The imbalance between high job demands and insufficient resources increases the risk of stress, burnout, and reduced service quality. Objective To examine the relationship between job demands and resources on work stress among healthcare workers. Method A systematic review using the PRISMA Framework was conducted on 13 national and international articles published between 2021–2025 that focused on job demands and resources in relation to work stress in the healthcare sector. Results Job demands such as physical workload, administrative tasks, emotional burden, and time pressure were found to increase stress, while resources including social support, supportive leadership, organizational policies, adequate facilities, and personal resources played a protective role. Conclusion The balance between job demands and resources determines the level of stress among healthcare workers. Integrated workload management strategies combined with strengthening organizational and personal resources are essential to maintain healthcare workers’ well-being and service quality. Keywords: Work stress, healthcare workers, job demands
Co-Authors Cahya Tri Purnami Putri Dwi Mulyani Shahuliyah, Zahroh Yekti Asih Purwestri