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Lignolytic Enzyme Activity of Isolated Bacteria from Termite (Coptotermes Sp.) and Milkfish (Chanos chanos Forsskal, 1775) Guts Latifah, Emi; Mulyani, Putri Dwi; Purwestri, Yekti Asih
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.19333

Bacteria BSR 2, Pseudomonas alcaligenes (BSR 3), Brevibacillus parabrevis (BSR 8), Brevibacillus sp. (BSR 9), isolated from termite gut and Bacillus licheniformis (BSA B1) isolated from milkfish gut have been known to possess celluloytic activity. However, their lignolytic ability has not been known. This study aimed to determine the lignolytic ability of bacteria isolated from termit (Coptotermes sp.) and milkfish (Chanos chanos Forsskal, 1775) guts and their enzymes characterization. The qualitative test was done through the spot test method, while quantitative assay was performed spectrophotometrically at 335 nm to calculate vanillin concentration. The isolates were grown in Lignin Mineral Medium, then the optical density (OD620) were measured every 24 hours for 5 days using spectrophotometer to determine their growth profile and the best isolation time of the lignolytic enzyme. Based on results, the best lignolytic enzyme isolation time for strains Bacillus licheniformis (BSA B1) and BSR 2 were 5 days, yielding lignolytic enzyme activity of 0.961 Â± 0.168 U/mg and 2.176 Â± 0.088 U/mg respectively, Â while strains Pseudomonas alcaligenes (BSR 3), Brevibacillus parabrevis (BSR 8), and Brevibacillus sp. (BSR 9) were 4 days, yielding of 1.206 Â± 0.045 U/mg, 1.162 Â± 0.191 U/mg, and 0.896 Â± 0.108 U/mg, respectively. The strain BSR 2 showed the highest lignolytic activity compared to other strains. The optimum temperature for lignolytic enzyme activity of BSR 2 was 30 â„ƒ and the optimum pH was 7. The lignolytic enzyme activity showed that these bacterial isolates can be a chance to be used as new alternative lignolytic enzyme source in commercial bioconversion process.

Amylolytic ability of bacteria isolated from termite (Coptotermes sp.) gut Putri Dwi Mulyani; Radhiyah Mardhiyah Hamid; Rifqi Zahroh Janatunaim; Yekti Asih Purwestri
Indonesian Journal of Biotechnology Vol 23, No 1 (2018)
Publisher : Universitas Gadjah Mada

BSR 2, BSR 3, BSR 8, and BSR 9, different bacteria isolated from the termite gut, have been shown to possess cellulolytic activities, but their amylolytic ability has heretofore been unknown. This study attempted to fill in this knowledge gap. The formation of a clear zone using the iodine test showed that the bacteria were able to produce and secrete amylase. Based on the results, the best cultivation times for strains BSR 2, BSR 3, BSR 8, and BSR 9 were 6, 3, 2, and 2 d, respectively, yielding amylase activities of 2.59 ± 0.13 U/mg, 2.00 ± 0.08 U/mg, 1.67 ± 0.10 U/mg, and 1.55 ± 0.12 U/mg, respectively. BSR 2 had the highest amylase activity compared with the other bacterial isolates. The optimum ph for bacterial amylase activity of BSR 2 was 7.0, and the optimum temperature was 40°C. The molecular characterization of isolates BSR 2, BSR 3, BSR 8, and BSR 9 was based on 16S rRNA gene sequences. Isolates BSR 8 and BSR 9 were thus identified as Brevibacillus parabrevis and Brevibacillus sp. With similarities amounting to 92.48% and 95.91%, while the BSR 3 isolate was identified as Pseudomonas alcaligenes with a similarity of 94.29%, and the BSR 2 isolate could not be identified yet.

Characterization of Lignocellulolytic Bacteria from Gut of Termite (Isoptera: Rhinotermitidae and Termitidae) Putri Dwi Mulyani; Muhammad Rizky Ulil Albab; Yekti Asih Purwestri
Jurnal Biologi Tropis Vol. 21 No. 2 (2021): Mei - Agustus
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i2.2737

A total of 10 bacterial isolates have been isolated from the gut of termites (Isoptera: Rhinotermitidae and Termitidae) and are known to have the ability to produce lignocellulolytic enzymes consisting of cellulase, laccase, and lignin peroxidase. The enzymatic ability allows these bacteria to be used as a source of new enzymes in the industrial world. However, further research on the character of bacteria to support identification has not been carried out. This study aims to characterize lignocellulolytic bacteria in the gut of termites morphologically and biochemically. Morphological observations were carried out including colony shape, colony edge, colony color, growth type, bacterial cell shape, and gram staining. Meanwhile, the biochemical characterization carried out included glucose fermentation test, indole formation, starch hydrolysis, catalase test, and nitrate reduction. The results showed that ten isolates of lignocellulolytic bacteria from the gut of termites (Isoptera: Rhinotermitidae and Termitidae) grew facultatively anaerobic, had almost similar morphological characters, with colony shapes including irregular and filamentous, colony edges in the form of lobate, undulate, and filamentous, and coloration. Colonies are white to yellowish white. The result of gram staining showed that most of the bacteria were gram positive bacteria with coccus and bacillus shaped bacterial cells. Biochemical analysis showed that these bacteria have the ability to ferment glucose, hydrolyze starch, reduce hydrogen peroxide (HÃ¢ââOÃ¢ââ 30%) and reduce nitrate.ÃÂ

Lignolytic Enzyme Activity of Isolated Bacteria from Termite (Coptotermes Sp.) and Milkfish (Chanos chanos Forsskal, 1775) Guts Emi Latifah; Putri Dwi Mulyani; Yekti Asih Purwestri
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.19333

Bacteria BSR 2, Pseudomonas alcaligenes (BSR 3), Brevibacillus parabrevis (BSR 8), Brevibacillus sp. (BSR 9), isolated from termite gut and Bacillus licheniformis (BSA B1) isolated from milkfish gut have been known to possess celluloytic activity. However, their lignolytic ability has not been known. This study aimed to determine the lignolytic ability of bacteria isolated from termit (Coptotermes sp.) and milkfish (Chanos chanos Forsskal, 1775) guts and their enzymes characterization. The qualitative test was done through the spot test method, while quantitative assay was performed spectrophotometrically at 335 nm to calculate vanillin concentration. The isolates were grown in Lignin Mineral Medium, then the optical density (OD620) were measured every 24 hours for 5 days using spectrophotometer to determine their growth profile and the best isolation time of the lignolytic enzyme. Based on results, the best lignolytic enzyme isolation time for strains Bacillus licheniformis (BSA B1) and BSR 2 were 5 days, yielding lignolytic enzyme activity of 0.961 Â± 0.168 U/mg and 2.176 Â± 0.088 U/mg respectively, Â while strains Pseudomonas alcaligenes (BSR 3), Brevibacillus parabrevis (BSR 8), and Brevibacillus sp. (BSR 9) were 4 days, yielding of 1.206 Â± 0.045 U/mg, 1.162 Â± 0.191 U/mg, and 0.896 Â± 0.108 U/mg, respectively. The strain BSR 2 showed the highest lignolytic activity compared to other strains. The optimum temperature for lignolytic enzyme activity of BSR 2 was 30 â„ƒ and the optimum pH was 7. The lignolytic enzyme activity showed that these bacterial isolates can be a chance to be used as new alternative lignolytic enzyme source in commercial bioconversion process.

Potential of Trichoderma harzianum as Environmentally Friendly Natural Pesticides for Controlling Phytophthora in Duku Plants Abdilla, Triyuni Verza; Simamora, Edenia Septefine; Amelia, Zahra Dini; Mulyani, Putri Dwi
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8611

Duku fruit production in Jambi has begun to decline, one of the causes is stem cancer. This stem cancer disease is caused by the fungus Phytophthora sp. The symptoms caused by this disease are dry and soft bark like cork, wilted leaves and falling off until the tree finally dies. The purpose of this study was to isolate Phytophthora sp., characterize the morphology of the pathogen and test the ability of Trichoderma to inhibit the growth of Phytophthora sp. The research was conducted by calculating the percentage of inhibition starting from sampling the organs of the duku plant and continuing to the isolation stage. The antagonistic test of Phytophthora sp. against Trichoderma was carried out using the dual culture method (dual culture). The results showed that Phytophthora sp. can be obtained from the bark of plants, with characteristics of colonies that are textured like cotton, white in color, round in shape with uneven edges. Microscopic observation of fungal isolates showed that Phytophthora sp. has morphological characteristics in the form of round-oval conidia, non-septate mycelium and branched conidiophores. Trichoderma has the ability to inhibit the growth of Phytophthora sp. with an inhibitory power of 63.52%.

Aplikasi Teknik Fermentasi Dalam Pembuatan Yoghurt Jeruk Nanas Kayu Manis (Jenaka) Di Kelurahan Aur Kenali Destiana, Anggari Linda; Nugraha, Anggit Prima; Husnudin, Uni Baroroh; Putri Dwi Mulyani , Putri Dwi Mulyani
ABDIMAS Madani Vol 6 No 2 (2024): Jurnal Abdimas Madani
Publisher : LPPM STIKES Madani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36569/abdimas.v6i2.163

Yoghurt merupakan minuman fungsional yang dikenal memiliki banyak manfaat. Manfaat tersebut dapat ditingkatkan dengan penambahan buah dan rempah. Jeruk, nanas, dan kayu manis merupakan buah dan rempah yang merupakan komoditas di Jambi yang mudah didapat, berkualitas, dan juga memiliki nilai gizi tinggi. Yoghurt dengan penambahan menggunakan sari buah atau ekstrak rempah mampumeningkatkan aktivitas antioksidan dan memberikan karakter fisik yang menarik. Pembuatan yoghurt dilakukan dengan menerapkan teknik fermentasi sederhana, sehingga diharapkan akan mudah dipraktikkan oleh ibu-ibu PKK. Kegiatan pengabdian ini bertujuan meningkatkan pengetahuan yang dimiliki mitra tentang cara pembuatan dan manfaat yoghurt jeruk, nanas, kayu manis. Kegiatan pengabdian kepada masyarakat ini dilakukan dalam bentuk penyampaian materi dan dilanjutkan dengan pelatihan atau praktikmembuat yoghurt secara langsung. Masyarakat yang mengikuti kegiatan pengabdian ini mengalami peningkatan pengetahuan yang dapat dilihat dari hasil post-test dan pre-test, dimana pada kategoripengetahuan baik meningkat dari 53,6% menjadi 78,6%. Berdasarkan hasil tersebut bisa disimpulkan bahwa kegiatan pengabdian ini meningkatkan pengetahuan masyarakat tentang teknik fermentasi yoghurt

Training on the Production of Biowash Derak at SMAN 3 Muaro Jambi : Pelatihan Pembuatan Biowash Derak (Detergen Organik Berbahan Dasar Lerak) di SMAN 3 Muaro Jambi Destiana, Anggari Linda; Nugraha, Anggit Prima; Husnudin, Uni Baroroh; Maritsa, Hasna Ul; Mulyani, Putri Dwi; Yusuf, Ashif Irvan
Dinamisia : Jurnal Pengabdian Kepada Masyarakat Vol. 9 No. 1 (2025): Dinamisia: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/dinamisia.v9i1.25254

The lerak fruit (Sapindus rarak) possesses significant potential as a natural detergent that can serve as an alternative to chemical-based detergents, which are increasingly concerning due to their detrimental effects on the environment and their impact on soil and aquatic organisms. The saponin content in lerak generates foam and exhibits antibacterial properties, making it environmentally safe for both soil and aquatic life. The objective of this program is to train students and teachers at SMAN 3 Muaro Jambi in producing natural detergent from lerak, thereby supporting extracurricular batik activities while also contributing to the establishment of an environmentally friendly school. The stages of this community service activity include socialization, hands-on training, and evaluation (pre- and post-tests). The results of the satisfaction measurement indicate that all participants were satisfied with the implementation of this community service, with 44.4% expressing high satisfaction and 55.6% being satisfied. The percentage of participant's knowledge regarding the name, benefits, preparation methods, and usage of lerak increased by 86%. The number of participants capable of producing lerak detergent rose significantly, from 4.7% to 85.4%, representing an increase of 80.7%. Overall, all participants expressed satisfaction with this activity and demonstrated an enhancement in their knowledge.

Potensi Berbagai Bahan Baku Biodiesel Berbasis Tanaman dan Limbah serta Mekanisme Transesterifikasi: Sebuah Narrative Literature Review Michelle Theona Kaban; Bambang Hariyadi; Ashif Irvan yusuf; Putri Dwi Mulyani
Jurnal Sains Dan Teknologi | E-ISSN : 3063-9980 Vol. 3 No. 1 (2026): Juli - September
Publisher : GLOBAL SCIENTS PUBLISHER

Show Abstract | Download Original | Original Source | Check in Google Scholar

Seiring meningkatnya permintaan energi dunia dan menurunnya cadangan bahan bakar fosil, biodiesel dikaji sebagai pilihan energi terbarukan. Penelitian ini berupaya mengevaluasi kemampuan berbagai bahan baku biodiesel serta proses produksinya melalui tinjauan literatur naratif. Diperiksa 15 artikel ilmiah selama sepuluh tahun terakhir dengan memfokuskan pada tipe bahan baku, metode produksi, tingkat efisiensi konversi, dan kualitas biodiesel. Penilaian potensi bahan baku meliputi kandungan minyak, efisiensi konversi, kemudahan budidaya, kompetisi dengan kebutuhan pangan, serta kepatuhan terhadap standar ASTM dan EN. Hasilnya mengindikasikan bahwa kelapa sawit dan mikroalga berada di peringkat teratas karena memiliki kadar minyak tinggi dan konversi yang efektif. Minyak bekas gorengan (jelantah) juga dianggap potensial karena biaya produksi rendah serta dampak lingkungan yang lebih kecil. Teknik transesterifikasi menjadi metode paling umum karena sederhana, efisien, dan memberikan hasil biodiesel tinggi. Pada intinya, biodiesel yang dihasilkan dari berbagai bahan baku dapat mendukung pengembangan energi terbarukan yang berkelanjutan.

Identifikasi Cendawan pada Buah Jeruk (Citrus Sp.) Impor dengan Metode Blotter Test Angel Dwi Cahyani Hasibuan; Anaiya Putri Adira; Kurnia Della Santri; Fitra Wahyuni; Putri Dwi Mulyani; Anggari Linda Destiana; Lidya Lidya
Jurnal Pendidikan Kimia, Fisika dan Biologi Vol. 2 No. 5 (2026): September: Jurnal Pendidikan Kimia, Fisika dan Biologi
Publisher : Asosiasi Riset Ilmu Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/jupenkifb.v2i5.1238

The increase in the volume of citrus fruit imports in Indonesia continues to increase every year, but this condition also carries the risk of pathogenic fungi that can reduce the quality and shelf life of the fruit. This study aims to detect and identify fungi in imported citrus fruit using the blotter test method. Samples of the Sunkist type (Citrus sp) originating from the United States and Wogan oranges (Citrus sp) originating from China were obtained from imported fruit markets and supermarkets in Jambi City. Samples of symptomatic fruit skin were surface sterilized, placed on moist filter paper in a sterile pertei dish and incubated at 21°C for 5-7 days. Fungal colonies were observed macroscopically using a stereo microscope (Olympus SZX2-ILLT) and microscopically using a compound microscope (Olympus BX 51 TF). Identification was carried out based on the morphological characteristics of the colonies and reproductive structures. The results showed three types of fungi in Sunkist oranges, namely Aspergillus sp., Penicillium sp., and Fusarium sp. Meanwhile, Colletotrichum sp., Fusarium sp., and Penicillium sp. were found in Wogan oranges. Thus, four fungal genera were successfully identified from both samples, with Penicillium sp. and Fusarium sp. as the most dominant genera, which are thought to have the ability to adapt to humid storage conditions. These results indicate that the blotter test method is proven effective in detecting and identifying fungi in imported citrus fruit based on their morphological characteristics. This blotter test procedure is very simple, relatively low cost, and short observation time.

Uji Efektivitas Ekstrak Tempe Kombinasi dari Kacang Kedelai dan Kacang Tanah sebagai Antibakteri terhadap Escherichia coli Dwi Meylani; Eymas Novellisa; Ega Shavina; Vindy Widiantari; Dugita Weni Setiani; Rhenaya Zara Sapitri; Putri Dwi Mulyani
Jurnal Cakrawala Pendidikan dan Biologi Vol. 3 No. 3 (2026): September: Jurnal Cakrawala Pendidikan dan Biologi
Publisher : Asosiasi Riset Ilmu Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/jucapenbi.v3i3.1229

Escherichia coli contamination in food products has created a need to identify natural biological preservatives. Fermentation of tempeh substrates is known to trigger the synthesis of bioactive compounds capable of inhibiting or killing microorganisms. This in vitro study aimed to evaluate the antibacterial activity of an ethanolic extract derived from a mixed-substrate tempeh (soybean and peanut in a 1:1 ratio) against E. coli ATCC 25922. Testing was conducted using the paper disc diffusion method at concentrations of 25%, 50%, and 75%, with amoxicillin serving as the positive control. Observations revealed mean clear zones of 6.33 ± 0.58 mm, 6.66 ± 1.15 mm, and 9.33 ± 1.15 mm for the 25%, 50%, and 75% concentrations, respectively. All extract treatments fell into the moderate inhibition category, whereas amoxicillin produced a zone of 7.67 ± 1.15 mm. The Kruskal-Wallis test yielded a p-value of 0.067 (p > 0.05), demonstrating that increasing the extract concentration did not result in a statistically significant difference in the extent of bacterial inhibition. It is concluded that the mixed-substrate tempeh extract shows preliminary potential for suppressing E. coli; however, varying the concentration up to 75% did not yield a significant increase in effectiveness.

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