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All Journal Journal of Fundamental and Applied Pharmaceutical Science Journal of Natural Product for Degenerative Diseases Indonesian Journal of Jamu
Hendig Winarno
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Medicine & Pharmacology Public Health

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The Isolation and Identification of Active Compound of Dendrophthoe praelonga (Blume) Miq. Extract Against Breast Cancer Cells (MCF-7) Junedi, M Irfan; Hendig Winarno; Hanafi, Muhammad; Artanti, Nina
Jurnal Jamu Indonesia Vol. 5 No. 3 (2020): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v5i3.117

Abstract

Petai mistletoe (Dendrophthoe praelonga (Blume) Miq) is semi-parasitic plant. Parasite plant has been used traditionally in Indonesia to prevent or treat degenerative diseases, such as cancer. The purpose of this study was to indentify anticancer compound of petai mistletoe leaves extract and to examine its antioxidant activity (based on DPPH method) and cytotoxic activity on breast cancer (MCF-7) cell lines in vitro using Alamar blue method. This research aims to isolating, characterize, determine antioxidant activity and cytotoxic activity. Separation was conducting column chromathography and characterize was conducted using TLC, FT-IR, LC-MS and NMR-1D. From the result obtained, the isolated compound is flavonol quercetine (3,3,7,3’,4’ pethahydroxyflavone) which active antioxidant with IC50 value of 9.4 μg/mL and the value of cytotoxic activity with IC50 750.62 μg/mL
Anti-inflammatory Activity of Glucomannan Resulting from β-mannanase Enzyme Hydrolysis in RAW 264.7 Cells Nuraini, Nuraini; Effionora Anwar; Dian Ratih Laksmitawati; Hendig Winarno; Siti Irma Rahmawati
Journal of Natural Product for Degenerative Diseases Vol. 2 No. 2 (2025): JNPDD March
Publisher : Faculty of Pharmacy Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58511/jnpdd.v2i2.7797

Abstract

Glucomannan is a polysaccharide of the hemicellulose type, composed of glucose and mannose chains. It has the ability to form solutions that expand and thicken. The bioactivity of carbohydrates is influenced by the length of their saccharide chains. Hydrolysis can alter the structural dimensions of polysaccharides, including molecular weight, type, number, and position of substituent groups, which in turn impacts their biological activity. Various methods can modify carbohydrate structures to enhance their value, with degradation being one such method. Enzymatic degradation is particularly advantageous due to its high specificity, efficiency, and minimal side effects. Numerous studies have identified glucomannan as having immunomodulatory effects on macrophage cells. When stimulated with lipopolysaccharides, macrophages release various cytokines and inflammatory mediators, such as TNF-α, iNOS, and NO. This study explored the potential anti-inflammatory effects of glucomannan (GM) and its enzymatically hydrolyzed form (Enz-GM) on RAW 264.7 cells. The research involved degrading glucomannan using the β-mannanase enzyme, followed by in vitro testing of the degradation product (Enz-GM) for anti-inflammatory activity. This was achieved using an ELISA kit to measure the expression of IL-6, TNF-α, and iNOS. The results showed that Enz-GM at pH 9 (Mw 1.24 kDa) significantly reduced the pro-inflammatory cytokines IL-6 and iNOS (p ≤ 0.05) compared to GM, while TNF-α expression did not show a significant difference between Enz-GM and GM.
Antioxidant Activity from Glucomannan Degradation Product Using β- mannanase Enzyme with DPPH Method Nuraini, Nuraini; Effionora Anwar; Dian Ratih Laksmitawati; Hendig Winarno; La Ode Akbar Rasydy; Fadila Helma Savira
Journal of Fundamental and Applied Pharmaceutical Science Vol. 6 No. 1 (2025): August
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/jfaps.v6i1.23778

Abstract

Antioxidants are defined as chemical components or compounds that can inhibit damage due to the oxidation process. Glucomannan, which is formed from a polysaccharide called mannan, has various benefits, such as anti-inflammatory, hypoglycemic, hypolipidemic, and antioxidant properties. This research aims to determine the antioxidant activity of glucomannan degradation using the β-Mannanase enzyme and the effect of temperature and pH on its antioxidant activity. Researchers carried out three degradation variations in the form of pH variations (5.5, 7, and 9) and temperature variations (45℃, 55℃, and 65℃), which resulted in 9 samples. The method used for antioxidant testing is DPPH. The antioxidant activity parameters were assessed based on the IC50 value obtained from the antioxidant test results. The best degradation results of glucomannan were pH 5.5 and a temperature of 65℃ with an IC50 value of 39.603 µg/mL due to the influence of pH and temperature on antioxidant activity, where low pH will have high antioxidants and high temperatures will cause a considerable reduction, resulting in high antioxidants.
Co-Authors Dian Ratih Laksmitawati Effionora Anwar Fadila Helma Savira Junedi, M Irfan Kusmardi Muhammad Hanafi Nina Artanti Nuraini Nuraini Pangartika Hikariastri Rahmawati, Siti Irma Rasydy, La Ode Akbar Syamsudin Abdillah