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All Journal Jurnal Entomologi Indonesia JCRS (Journal of Community Research and Service)
Sirait, Desianty Dona Normalisa
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Social Sciences

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MORPHOLOGY VARIATION OF METARHIZIUM ANISOPLIAE FROM SOIL IN NORTH SUMATRA AREAS Sirait, Desianty Dona Normalisa; Tobing, Maryani Cyccu; Safni, Irda
Journal of Community Research and Service Vol 1, No 2: July 2017
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jcrs.v1i2.9627

Abstract

AbstractMetarhizium anisopliae is an important entomopathogenic fungus that mainly used for biological control. Isolates of M. anisopliae extracted from soil from 15 oil palm plantations located in several districts on the Province of North Sumatra, Indonesia have been investigated to identify their morphological diversity. The morphology of assessed fungi was constructed in the the form of conidia, conidia size, color and shape colony and growth diameter of the 15 isolates. All of conidia forms showed cylindrical shape and string together. The length/width (μm) ratio of conidia size was minimum 2,16 and maximum 3,54. The highest growth rate isolates were found in M3 (Bandar Selamat) and M12 (Mancang) treatment and the lowest rate was M11 (Teluk Parit Kaca) treatment. The color of colonies were varied among the isolates. They were greenish yellow, dark green, yellowish and showing zonation/no zonation.Keywords : Metarhizium anisopliae, biological character, morphological diversity
MORPHOLOGY VARIATION OF METARHIZIUM ANISOPLIAE FROM SOIL IN NORTH SUMATRA AREAS Sirait, Desianty Dona Normalisa; Tobing, Maryani Cyccu; Safni, Irda
Journal of Community Research and Service Vol. 1 No. 2: July 2017
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jcrs.v1i2.9627

Abstract

AbstractMetarhizium anisopliae is an important entomopathogenic fungus that mainly used for biological control. Isolates of M. anisopliae extracted from soil from 15 oil palm plantations located in several districts on the Province of North Sumatra, Indonesia have been investigated to identify their morphological diversity. The morphology of assessed fungi was constructed in the the form of conidia, conidia size, color and shape colony and growth diameter of the 15 isolates. All of conidia forms showed cylindrical shape and string together. The length/width (μm) ratio of conidia size was minimum 2,16 and maximum 3,54. The highest growth rate isolates were found in M3 (Bandar Selamat) and M12 (Mancang) treatment and the lowest rate was M11 (Teluk Parit Kaca) treatment. The color of colonies were varied among the isolates. They were greenish yellow, dark green, yellowish and showing zonation/no zonation.Keywords : Metarhizium anisopliae, biological character, morphological diversity
Keragaman genetik cendawan entomopatogen Metarhizium anisopliae (Metsch.) berasal dari tanah pertanaman kelapa sawit berdasarkan penanda RAPD: Genetic diversity of the entomopathogenic Metarhizium anisopliae (Metsch.) from oil palm planting soil based on RAPD markers Sirait, Desianty Dona Normalisa; Tobing, Maryani Cyccu; Safni, Irda
Jurnal Entomologi Indonesia Vol 20 No 1 (2023): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.20.1.22

Abstract

Metarizhium anisopliae (Metsch.) is an entomopathogenic fungus that attacks Oryctes rhinoceros (Linnaeus). This fungus is one of the potential bioagents that is environmentally friendly. The purpose of this study was to isolate the fungus M. anisopliae from various oil palm plantation soil samples and to see its genetic diversity based on RAPD markers. The method used in this research is the morphological and the molecular identification of M. anisopliae (ITS), and genetic diversity testing using RAPD markers. Fifteen isolates of M. anisopliae isolated from 15 oil palm plantation sites in North Sumatra were examined for their molecular diversity. On the basis of morphological characteristics, all isolates were identified as M. anisopliae. The result of sequencing with the ITS primer showed that all isolates of M. anisopliae isolated from soil had a 85–99% homology of with M. anisopliae and M. brunneum reference strains from gen bank database National Center Biotechnology Information (NCBI). Eight RAPD primers generated 39 scorable bands which 38 (98,44%) of them were polymorphic. Clustering analysis was performed based on RAPD profiles using Neighbour-Joining Tree method that formed 3 groups.
Co-Authors Irda Safni Maryani Cyccu Tobing