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Potensi Jamur Entomopatogen untuk Mengendalikan Ulat Grayak Spodoptera litura F. pada Tanaman Tembakau In Vivo Masyitah, Irna; Sitepu, Suzanna F; Safni, Irda
Jurnal Agroekoteknologi Vol 5, No 3 (2017)
Publisher : Universitas Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jaet.v5i3.18316

Spodoptera litura is an important pest for tobacco. The objective of this study was to determine the potency of entomopathogenic fungi at various conidia density against Spodoptera litura on tobacco. This study was conducted in screen house, Faculty of Agriculture, University of Sumatera Utara from October until February 2016. This study was used a randomized block design (RBD) with two factors and three replications. The first factor was the conidia density (106, 107, 108 conidia/ml), the second factor was the type of fungi (Metarhizium anisopliae, Beauveria bassiana, and Lecanicillium lecanii) The result showed that: the highest larval mortality was at concentration of 108 conidia/ml (73.33%). The highest larval mortality was on M. anisopliae (57.78%) followed by B. bassiana (51.11%) and L. lecanii (40.00%). The infected larvae showed slow movements, color changes to paleness, decreased appitite, over time silent and die. The dead larva body hardened and blackened. The highest intensity of fungal infection was at concentration of 106 conidia/ml (42.22%). Key words: entomopathogenic fungi, Spodoptera litura, tobacco

RESISTANCE OF SEVERAL RICE VARIETIES AGAINST THE BACTERIAL PANICLE BLIGHT DISEASE (BURKHOLDERIA GLUMAE) Wahidah, Nurni; Safni, Irda; Hasanuddin, Hasanuddin; Lisnawita, Lisnawita
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 19, No 1 (2019): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.11915-22

Resistance of Several Rice Varieties against the Bacterial Panicle Blight Disease (Burkholderia glumae) .Burkholderia glumae is included as A2 Quarantine Plant Pest Organism, which is found in some restricted area in Indonesia. B. glumae is a seedborne pathogen that causes panicle blight, seedling rot, grain rotand leaf sheath browning on rice plants. This research was objected to determine the resistance of five rice varieties against bacterial panicle blight pathogen. The experiment was carried out at the screen house of Faculty of Agriculture, Universitas Sumatera Utara, Medan from May to September 2018. This experiment was designed using Factorial Completely Randomized Design (CRD) with 2 factors; Factor 1: B1 (sterile water as negative control), B2 (B. glumae isolate CH BJ), B3 (B. glumae isolate IR 64), B4 (B. glumae isolate IC PRC), B5 (B. glumae isolate DSMZ 9512? as positive control). Factor 2: V1 (Cisokan variety), V2 (Inpari 4 variety), V3 (Situbagendit variety), V4 (Inpari 32 variety) and V5 (Cidenu variety). The results showed that all five rice varieties were highly susceptible to bacterial panicle blight pathogen. Inpari 32 variety had the highest number of seeds and weight of 100 seeds and had the lowest percentage of empty seeds. IR 64 variety had the lowest number of seeds and the weight of 100 seeds and had the highest percentage of empty seeds.

Uji Antagonis Tanaman Bangun – Bangun (Plectranthus amboinicus Lour) sebagai Fungisida Nabati terhadap Jamur Akar Putih (Rigidoporus microporus Swartz) di Laboratorium dan di Lapangan Neny Yanti Siregar; Lahmuddin Lubis; Irda Safni; Cici Indriani Dalimunthe
Jurnal Agroekoteknologi Vol 5, No 2 (2017)
Publisher : Universitas Sumatera Utara

Penelitian ini bertujuan untuk mendapatkan bahan dan konsentrasi ekstrak yang optimal di laboratorium dan mengetahui potensi daya hambat tanaman bangun-bangun terhadap patogen penyebab penyakit jamur akar putih (Rigidoporus microporus) secara langsung pada stum karet di polibeg. Penelitian ini dilaksanakan di laboratorium dan areal rumah kassa Balai Penelitian Sungei Putih dengan ketinggian tempat 80 m dpl, dimulai bulan September 2015 sampai Januari 2016. Penelitian di laboratorium menggunakan Rancangan Acak Lengkap (RAL) non faktorial dengan 13 Perlakuan dan 3 ulangan. Perlakuan jenis bahan ekstrak adalah daun dan akar kering serta daun dan akar segar sedangkan konsentrasi ekstrak yaitu 2,5; 5; 7,5% serta tanpa perlakuan (kontrol). Penelitian di lapangan menggunakan Rancangan Acak Kelompok (RAK) faktorial dengan dua faktor yaitu faktor jumlah tanaman bangun-bangun (0,2,4, dan 8) dan skala JAP (1dan 2) sehingga diperoleh 8 kombinasi perlakuan dan 3 ulangan. Hasil terbaik di laboratorium didapat pada perlakuan ekstrak akar kering metanol (A8 dan A9) dengan konsentrasi 5% dan 7,5% yang memiliki daya hambat terbesar dalam menekan pertumbuhan JAP yaitu 74,00 % dan 75,67%. Hasil terbaik di lapangan adalah perlakuan T1S1 yaitu 2 tanaman bangun-bangun dengan skala JAP 1 yang dapat menurunkan skala JAP hingga 0 pada stum karet.

Potensi Jamur Entomopatogen Untuk Mengendalikan Hama Ulat Grayak (Spodoptera litura) Fabricius (Lepidoptera : Noctuidae) Pada Tanaman Tembakau di Rumah Kasa Irna Masyitah; Suzanna Fitriany Sitepu; Irda Safni
Jurnal Agroekoteknologi Vol 5, No 3 (2017)
Publisher : Universitas Sumatera Utara

Spodoptera litura is an important pest for tobacco. The objective of this study was to determine the potency of entomopathogenic fungi at various conidia density against Spodoptera litura on tobacco. This study was conducted in screen house, Faculty of Agriculture, University of Sumatera Utara from October until February 2016. This study was used a randomized block design (RBD) with two factors and three replications. The first factor was the conidia density (106, 107, 108 conidia/ml), the second factor was the type of fungi (Metarhizium anisopliae, Beauveria bassiana, and Lecanicillium lecanii) The result showed that: the highest larval mortality was at concentration of 108 conidia/ml (73.33%). The highest larval mortality was on M. anisopliae (57.78%) followed by B. bassiana (51.11%) and L. lecanii (40.00%). The infected larvae showed slow movements, color changes to paleness, decreased appitite, over time silent and die. The dead larva body hardened and blackened. The highest intensity of fungal infection was at concentration of 106 conidia/ml (42.22%).

Uji Efektivitas Pengendalian Kimiawi dan Biologi terhadap Hama Penggerek Polong (Maruca testulalis Geyer.) pada Tanaman Kacang Hijau winda arthadinatha lingga; Irda Safni
Jurnal Agroekoteknologi Vol 7, No 1,Jan (2019)
Publisher : Universitas Sumatera Utara

Penelitian ini bertujuan untuk menguji keefektifan penggunaan λ-sihalotrin, ekstrak daun babadotan, dan Bacillus thuringiensis (Bt) komersial dalam mengendalikan hama penggerek polong tanaman kacang hijau di lapangan. Penelitian ini dilaksanakan di desa Klambir V, Kecamatan Hamparan Perak, Kabupaten Deli Serdang dengan ketinggian ± 25 m dpl. Penelitian ini menggunakan Rancangan Acak Kelompok non faktorial, terdiri dari 6 perlakuan yaitu : Kontrol, tanpa pengendalian (28 HST sampai panen), λ-sihalotrin 2 ml/l air seminggu sekali aplikasi (pada 35 HST sampai dengan 56 HST), λ-sihalotrin 2 ml/l air 2 kali aplikasi (35 HST dan 49 HST), ekstrak daun gulma babadotan 50 gr/l air dan Bt komersial (Thuricide HP) 5gr/l air, dengan 4 ulangan. Hasil penelitian menunjukkan bahwa rata-rata persentase polong terserang tertinggi terdapat pada perlakuan tanpa pengendalian sejak 28 HST sampai panen (33,77%) dan terendah pada perlakuan penggunaan insektisida λ-sihalotrin 2 ml/l air seminggu sekali pada 35-56 HST (20,45%). Penggunaan ekstrak daun gulma babadotan 50 gr/l air mampu mengurangi jumlah biji yang terserang hama polong. Produksi kacang hijau tertinggi terdapat pada perlakuan penggunaan insektisida λ-sihalotrin 2 ml/l air seminggu sekali pada 35-56 HST (1,758 Kg) dan terendah pada perlakuan tanpa pengendalian selama pertumbuhan tanaman (0,798 Kg).

Effectivity assay of several PGPR strains for controlling collar rot disease (Athelia rolfsii (Curzi)) on soybean (Glycine max (L.) Merril). Widya Antastia; Irda Safni; Ameilia Zuliyanti Siregar
Jurnal Agroekoteknologi Vol 7, No 2,April (2019)
Publisher : Universitas Sumatera Utara

A. rolfsii (Curzi) is a cosmopolitan fungus which infects a large varieties of plants, especially the young one. This study was aimed to test the effectivity of 5 types of plant growth promoting rhizobacteria (PGPR) in suppressing the growth of Athelia rolfsii (Curzi) and to obtain the most effective type of PGPR for controlling disease severity and incidence A. rolfsii. This research was conducted at Plant Disease Laboratory and at the screen house at Faculty of Agriculture, Universitas Sumatera Utara, Medan from March 2017 until August 2017. The research used by non Factorial Randomized Block Design with 7 treatment combinations and 4 replications: R0 (Control), R1 (A. rolfsii), R2 (A. rolfsii+Aeromonas hydrophila), R3 (A. rolfsii+Burkholderia cepacia), R4 (A. rolfsii+Serratia ficaria), R5 (A. rolfsii+Pantoea spp. 2), R6 (A. rolfsii+Vibrio alginolyticus). The results showed that the application of B. cepacia, Pantoea spp. 2, and V. Alginolyticus could suppress the growth of A. rolfsii, with disease incidence was 0.00 % for treatments of B. cepacia, Pantoea spp. 2, and V. alginolyticus compared to the treatment (only A. rolfsii), which was 100.00 %. The disease severity of treatments of B. cepacia, Pantoea spp. 2, and V. alginolyticus was 0.00 % compared to the treatment (only A. rolfsii), which was 50.00 %.

Pengujian bakteri endofit asal cabai dalam menekan pertumbuhan F. oxysporum f.sp. capsici penyebab penyakit layu fusarium pada cabai Irma Handayani Sihombing; Mukhtar Iskandar Pinem; Irda Safni
Jurnal Agroekoteknologi Vol 7, No 2,April (2019)
Publisher : Universitas Sumatera Utara

ABSTRACTEndophytic bacteria are bacteria in plant tissues that do not cause disturbance in plants and can increase plant resistance to pests and pathogens as well increase plant growth. This study was aimed to determine the ability of endophytic bacteria isolated from chili plants in suppressing the development of fusarium wilt disease caused by F. oxysporum f.sp. capsici and utilize the capability of several carriers as a medium for growing endophytic bacteria to be stored, transported and applied. Endophytic bacteria are bacteria in plant tissues that do not cause disturbance in plants and can increase plant resistance to pests and pathogens and increase plant growth. This study was aimed to determine the ability of endophytic bacteria isolated from chili plants in suppressing the development of fusarium wilt disease caused by fungus F. oxysporum f.sp. capsici. The in vitro antagonistic test results showed that the most effective endophytic bacteria againts isolate of F. oxysporum f.sp. capsici was BE 12 isolate (20.41%). Isolate BE 12 which reacts positively to the fluorescence test has an effect in inhibiting the growth of mycelium from fungus F. oxysporum f.sp. capsici because the endophytic bacteria belong to P. fluorescens produces pyoluteorin and pyrrolnitrin compounds that are toxic to pathogen. The characteristic of morphology of all endophytic bacterial isolate a were circular colony with white colour,and flat elevation and undulate margin. Isolates of BE 2, BE 4 and BE 12 were Gram negative, while the other were Gram positive.

Spread of bacterial wilt disease on potato in three villages in Silimakuta District, Simalungun, North Sumatra, Indonesia Nahrisa Rahmadhani; Mukhtar Iskandar Pinem; Irda Safni
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 22 No. 2 (2022): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.222162-167

Bacterial wilt disease caused by Ralstonia syzygii subsp. indonesiensis is an important disease in potatoes. This disease is a soil-borne pathogen that is difficult to control and becomes an obstacle in potato production. This study aimed to determine the distribution of bacterial wilt disease in potatoes caused by R. syzygii subsp indonesiensis in three villages in Silimakuta District, Simalungun Regency, North Sumatra. This research was exploratory research that was conducted using a survey method by calculating the incidence of disease in potatoes in 9 locations from three villages. The results showed that the intensity of bacterial wilt occurred with different percentages of disease incidence. The highest disease incidence of 100% was in Sibangun Meriah Village Location 2 and the lowest disease incidence of 22,5% was in Saribu Dolok Village Location 1.

Keragaman genetik cendawan entomopatogen Metarhizium anisopliae (Metsch.) berasal dari tanah pertanaman kelapa sawit berdasarkan penanda RAPD: Genetic diversity of the entomopathogenic Metarhizium anisopliae (Metsch.) from oil palm planting soil based on RAPD markers Desianty Dona Normalisa Sirait; Maryani Cyccu Tobing; Irda Safni
Jurnal Entomologi Indonesia Vol 20 No 1 (2023): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.20.1.22

Metarizhium anisopliae (Metsch.) is an entomopathogenic fungus that attacks Oryctes rhinoceros (Linnaeus). This fungus is one of the potential bioagents that is environmentally friendly. The purpose of this study was to isolate the fungus M. anisopliae from various oil palm plantation soil samples and to see its genetic diversity based on RAPD markers. The method used in this research is the morphological and the molecular identification of M. anisopliae (ITS), and genetic diversity testing using RAPD markers. Fifteen isolates of M. anisopliae isolated from 15 oil palm plantation sites in North Sumatra were examined for their molecular diversity. On the basis of morphological characteristics, all isolates were identified as M. anisopliae. The result of sequencing with the ITS primer showed that all isolates of M. anisopliae isolated from soil had a 85–99% homology of with M. anisopliae and M. brunneum reference strains from gen bank database National Center Biotechnology Information (NCBI). Eight RAPD primers generated 39 scorable bands which 38 (98,44%) of them were polymorphic. Clustering analysis was performed based on RAPD profiles using Neighbour-Joining Tree method that formed 3 groups.

Kelimpahan Jamur Non-Patogenik Pada Rhizosfer Daerah Endemik Patogen Magnaporthe grisea Penyebab Penyakit Blas Pada Tanaman Padi (Oryza sativa L.) Faqih Aulia Rahman; Irda Safni; Lisnawita Lisnawita
Agro Bali : Agricultural Journal Vol 6, No 2 (2023)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v6i2.1145

Penelitian ini bertujuan untuk mengetahui kelimpahan jamur yang terdapat pada rhizosfer daerah endemik patogen Magnaporthe grisea penyebab penyakit blas pada tanaman padi. Penelitian ini merupakan penelitian awal yang dimulai dengan melakukan kegiatan survei pada beberapa lokasi endemik dan menentukan lokasi yang paling tepat dijadikan sebagai lokasi pengambilan sampel tanah untuk mendapatkan kelimpahan jamur non- patogenik. Dari 3 lokasi yang dilakukan survei, diantaranya Sukamandi Hilir, Sendayan dan Secanggang diketahui bahwa daerah Sendayan merupakan lokasi yang paling tepat untuk dijadikan sebagai lokasi penelitian sebab terdapat gejala serangan blas pada tanaman padi di seluruh lahan budidaya padi. Setelah dilakukan pengambilan sampel secara acak spesifik lokasi, selanjutnya dilakukan purifikasi pada jamur yang terdapat pada sampel tanah yang telah dihomogenkan. Pengujian patogenisitas pada jamur dilakukan dengan mengisolasi jamur yang dibiakkan pada media PDB ke irisan kentang yang telah dilakukan sterilisasi permukaan. Kesimpulan penelitian ini adalah 1) Terdapat 13 isolat jamur pada lokasi penelitian, 2) Setelah dilakukan identifikasi secara morfologi mikroskopik dan makroskopik ditemukan 3 genus jamur yang terdapat pada rhizosfer daerah endemik blas, diantaranya Aspergillus, Penicillium dan Mucor, 3) Aspergillus merupakan genus jamur yang paling berlimpah pada daerah endemik blas padi.

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