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Antibacterial assessment of Ziziphus mauritiana Lam on inhibition of the growth and biofilm of Streptococcus mutans Syafriza, Dharli; Hasanah, Uswatun; Hidayatullah, Taufiqi; Rifki, Ayudia; Gani, Basri A.
Dental Journal Vol. 57 No. 3 (2024): September
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v57.i3.p189-194

Abstract

Background: Streptococcus mutans (S. mutans) causes dental caries. S. mutans biofilms are inhibited by the antibacterial properties of Ziziphus mauritiana Lam (Z. mauritiana Lam). Purpose: Evaluating the potential of Z. mauritiana Lam in inhibiting the growth and biofilm formation of S. mutans ATCC 25175 in vitro. Methods: This study used the ethanolic extract of Z. mauritiana Lam as the test material and S. mutans as the research subject. Spectrophotometry (620 nm) was used to assess the growth of S. mutans, the inhibition of S. mutans biofilm using a 1% crystal violet staining was measured by spectrophotometry (520 nm), and the visualization of the biofilm mass was conducted with an electric microscope (200x). Results: At all concentrations, Z. mauritiana Lam displayed excellent growth inhibition of S. mutans 0.04–0.09 (< 300 CFU/mL) and was able to inhibit the formation of S. mutans biofilm with a strong scale of optical density (OD) 0.4 at 24 hours incubation time. At the same time, the incubation time of 48 and 72 hours tended to have moderate-scale biofilm inhibition (OD 0.2–3.9). At a concentration of 25%, the biofilm mass decreased by a relatively small size, the same as the positive control group. At 50%, 12.5%, and 6.25%, it was seen that the S. mutans biofilm mass experienced a dominant loss. Conclusion: Z. mauritiana Lam can be bacteriostatic against the growth of S. mutans and can inhibit the formation of biofilms by degrading the structure and mass of S. mutans biofilms.
Primary second molar relations in preschool-aged children Arifin, Rafinus; Hidayatullah, Taufiqi; Rahayu, Sholeha
Padjadjaran Journal of Dentistry Vol 30, No 1 (2018): March 2018
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (189.404 KB) | DOI: 10.24198/pjd.vol30no1.16113

Abstract

Introduction: The primary second molar relation is also called the terminal plane, which is the relation between the distal surface of the maxillary and mandibular second primary molars in a centric occlusion position. One of the most important characteristics of the ideal occlusion development on the primary dentition is if the primary second molar relation classified as the flush terminal plane. This relation plays a significant role in determining the relation in permanent molar because the eruption of the first permanent molars are guided by the distal surface of the maxillary and mandibular second primary molars. The purpose of this study was to found out the clinical description of the primary second molar relation among the preschool-aged children in four kindergartens in Banda Aceh. Methods: This study was a descriptive study with cross-sectional study approach. The subject selection method was performed with a purposive sampling method involving as much as 37 subjects, which were students of State Kindergarten of Pembina 1, State Kindergarten 2, State Kindergarten 3, and State Kindergarten of Adidarma 4, Banda Aceh. The examination was performed by using the intraoral mirror when the teeth were in the centric occlusion position. Decisions regarding the primary second molar relation were made by the bilateral occlusion (left and right). Results: The flush terminal plane was found in 45.9% of all respondents, the mesial step was 54.1%, and the distal step was 0.0%. Conclusion: The primary second molar relation among preschool-aged children generally had the mesial step.