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Centrifugation effect on size distribution of organic dental filler made from glutinous rice flour Takarini, Veni; Asri, Lia Amelia Tresna Wulan; Djustiana, Nina; Hadi, Bambang Kismono
Padjadjaran Journal of Dentistry Vol 34, No 2 (2022): July
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol34no2.40711

Abstract

ABSTRACTIntroduction: Organic fillers made from the green-synthesis process can be utilized in dental treatment due to their non-toxic characteristic of the oral environment. Dental fillers must be smaller to improve their mechanical, physical, and biological properties. Material processing and centrifugation contribute to the size of processed materials. This study evaluates the effect of different centrifugation speeds on the size and distribution of glutinous rice flour as organic dental filler. Methods: This experimental study uses glutinous rice flour suspended in water and then centrifuged at different speeds of 3000 and 6000 rpm compared to untreated glutinous rice flour as control. The particle size was examined using Scanning Electron Microscope (SEM) and Dynamic Light Scattering (DLS). Next, Fourier Transform Infra Spectroscopy (FTIR) and X-ray Diffraction (XRD) were performed to evaluate whether there were structure and crystallinity changes. Finally, the cytocompatibility of fillers was examined by a cell viability test. Results: Centrifugation of glutinous rice flour at 3000 rpm resulted in smaller average size particles, evenly distributed, and higher crystallinity than other groups. There were no changes in functional groups, as shown by FTIR spectra. However, the processed samples were more amorphous compared to the control. Importantly, the processed samples appeared to be non-toxic, with a range of cell viability of 82.75-86.67% after 24 h incubation. Conclusion: Centrifugation speed of 3000 rpm has decreased in size, and a more homogenous particle size of organic filler made from glutinous rice flour. Importantly, it improved the cytocompatibility of particles compared to untreated control. Altogether, we proposed this organic material as a new potential material in the dental application that can be further investigated. Keywords: centrifugation speed; size distribution; organic dental filler; glutinous rice flour
In vivo histomorphological evaluation of geopolymer-carbonated apatite nanocomposites implanted on rabbit tibia at early bone healing Sutanto, Dahlia; Satari, Mieke Hemiawati; Hernowo, Bethy Suryawathy; Priosoeryanto, Bambang Pontjo; Septawendar, Rifki; Asri, Lia Amelia Tresna Wulan; Purwasasmita, Bambang Sunendar
Padjadjaran Journal of Dentistry Vol 33, No 1 (2021): March 2021
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol33no1.28899

Abstract

Introduction: Dental implants have become a more desirable treatment for replacing missing teeth. The mechanical properties and biocompatibility of titanium and zirconia are excellent, but they are less bioactive. The chemical composition of the carbonate apatite is similar to enamel and dentin. Geopolymers are inorganic polymers, and they are similar to ceramics. They have excellent mechanical properties, bioactivity, biocompatibility. The purpose of this study was to assess histomorphological evaluation of geopolymer-carbonated apatite nanocomposites implanted on rabbit tibia at early bone healing in vivo. Methods: Geopolymer-CHA nanocomposites with a diameter of 3 mm and length of 6 mm was placed in the tibia of eight male New Zealand White rabbit whose body weight is 3 to 3.5 kg and six-month ages. Experimental subjects were randomly assigned to 2 groups for assessing the bone healing capability around samples to 14 and 28 days histomorphologically. Wilcoxon test was performed, and p<0.05 was considered significant, using Minitab software version 13. Results: Granulation tissue, woven, and lamellar bone was analysed. A reactive bone formation was revealed in the 14th day. Osteoblasts, osteoids, and osteocytes showed more mature and woven bone became denser on the 28th day. Conclusion: Geopolymer-CHA nanocomposites could be considered a candidate for dental implant material from this histomorphological evaluation.
A Review of Valorization of Okara and Okara Nanocelullose Derived from Soybean Industrial Waste for Biosorbent Applications Puspitasari, Dita; Asri, Lia Amelia Tresna Wulan; Syarif, Nur Fadhilah
Mesin Vol 29 No 2 (2023)
Publisher : Faculty of Mechanical and Aerospace Engineering

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/MESIN.2023.29.2.2

Abstract

Solid waste from tofu production, also known as okara, has numerous applications and can produce high-value-added products. However, the utilization of okara in Indonesia is not yet significant. This written work proposes okara as a source of nanocellulose to produce wastewater adsorbents in freshwater by fabricating it as aerogels and hydrogels. Wastewater is highly hazardous to the environment and living organisms as it can contain saturated salts, heavy metals, organic compounds, oil emulsions, dyes, and even microbes as pollutants that can lead to various diseases or death. Therefore, research on biosorbents is always a hot topic. Biosorption is the process of binding metal ions into the cellular structure of biological materials. Lignocellulosic biosorbents have high adsorption properties due to their ion exchange capability. Okara biosorbent can increase the absorption capacity of Pb2+ ions by up to 20% compared to conventional absorbents. The soybean skin component could also remove contaminated textile dyes from water. Additionally, the low lignin content in okara makes it easier to utilize than other lignocellulosic materials. This research study also shows that okara-based nanocellulose aerogels can maintain their shape or exhibit full shape recovery properties even after being used repeatedly.
Potential of Chitosan-Encapsulated Mango Leaf Extract Nanoemulsion as Fruit Packaging Putra, Panji Setya Utama; Adhika, Damar Rastri; Asri, Lia Amelia Tresna Wulan
Chimica et Natura Acta Vol 13, No 3 (2025)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v13.n3.56202

Abstract

Fruits have a relatively short shelf life. One of the extensively researched preservation methods for fruits is edible coating. Edible coating is a method of coating food products with a polymer layer. Considering the environmental and sustainability aspects, edible coatings were synthesized from biodegradable polymers derived from polysaccharides. Chitosan is a widely developed and researched type of biopolymer due to its antimicrobial activity. In addition to antimicrobial activity, antioxidant properties are important in food packaging applications. Mango leaves (Mangifera indica L.) contain various secondary metabolites that play a role in antioxidant activity, such as mangiferin, gallic acid, flavonoids, and gallotanin. In this study, mango leaf extract nanoemulsion was synthesized to improve the antioxidant and anti-UV properties of biopolymer chitosan-based fruit packaging. Mango leaf extract nanoemulsion was synthesized using surfactant and ultrasonication methods. The results of Particle Size Analyzer (PSA) measurements showed that the size of all nanoemulsions was 83-99 nm. Observations with Transmission Electron Microscope (TEM) showed that the nanoemulsion was spherical with a 15-46 nm size range. The UV-Vis spectrophotometer instrument was used to test antioxidant properties using the DPPH reagent and anti-UV activity. These tests showed that adding mango leaf extract concentration to the nanoemulsion system increased DPPH inhibition and absorbance against UV light. The results of the performance test of mango leaf extract nanoemulsion encapsulated by chitosan have a high potential to be used as a bio-based edible coating matrix and a substitute for materials that are difficult to degrade.