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All Journal Journal of Degraded and Mining Lands Management BioEksakta : Jurnal Ilmiah Biologi Unsoed
Andriani, Lutfi Tri
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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Isolasi dan Karakterisasi Cendawan Pathogen Daun Jagung Manis (Zea mays) Varietas Talenta menggunakan Metode Direct Plating Dan Moist Chamber di BBPP Ketindan, Jawa Timur fadilah, nurul; Rahayu, Yuni Sri; Andriani, Lutfi Tri
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 1 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3777

Abstract

Permintaan pasar akan jagung meningkat dengan seiring berjalannya tahun. Permintaan terbesar dari semua jenis jagung adalah jagung manis (Zea mays) varietas talenta. Namun, produksi dalam negeri tidak mencukupi permintaan jagung tersebut. Hal ini dikarenakan adanya masalah seperti, penyakit jagung yang menggagalkan hasil panen secara drastis. Penyakit ini disebabkan oleh cendawan patogen. Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi cendawan patogen pada daun jagung manis (Zea mays) varietas Talenta yang diambil dari Lahan Pertanian, Balai Besar Pelatihan Pertanian (BBPP) Ketindan, Jawa Timur. Penelitian ini merupakan penelitian eksperimental menggunakan Rancangan Acak lengkap (RAL). Metode isolasi cendawan yang digunakan adalah direct plating dan moist chamber. Karakterisasi dan Identifikasi berdasarkan karakter makroskopis meliputi: bentuk koloni tua, warna koloni tua, tekstur koloni tua, bentuk koloni muda, warna koloni muda, dan tekstur koloni muda. Karakter mikroskopis meliputi dinding hifa, tipe percabangan hifa, ada tidaknya septa, ada tidaknya sambungan apit (clamp connection), bentuk sporangium, warna sporangium, bentuk spora, warna spora). Hasil isolasi cendawan dari metode direct plating didapatkan 5 spesies cendawan patogen antara lain: Helminthosporium sp., Rhizoctonia sp., Peronosclerospora spp., Puccinia sp., dan Trichometaspheria sp., sedangkan metode moist chamber didapatkan 2 spesies cendawan patogen yaitu: Helminthosporium sp. dan Rhizoctonia sp.
Glyphosate biodegradation by plant growth promoting bacteria and their effect to paddy germination in glyphosate contaminated soil Andriani, Lutfi Tri; Aini, Luqman Qurrata; Hadiastono, Tutung
Journal of Degraded and Mining Lands Management Vol. 5 No. 1 (2017)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2017.051.995

Abstract

Glyphosate is the most widely used herbicide in Indonesia. Glyphosate persistence between 55 days to 3 years. Widespread and uncontrolled use can cause weeds to become resistant and residue contaminates the soil and water environment. Due to the residual impact of glyphosate, it is necessary to identify a method that can increase the degradation of glyphosate. Several studies have shown that glyphosate can be degraded by microorganisms (fungi, rhizosphere and endophytic bacteria), some of which are members of plant growth-promoting bacteria. This study used the bacteria Enterobacter cloacae, Enterobacter sp and Pseudomonas fluorescens. These three types of bacteria have growth-promoting properties and potentially increase glyphosate degradation. Results of chromatogram on the residual test of glyphosate in liquid medium and soil containing glyphosate showed that glyphosate residue decreased with the addition of bacterial treatment when compared to control. The percentage of degradation in liquid medium are 96.06%  by Enterobacter cloacae, 57% by Enterobacter spand 93.45%  by Pseudomonas fluorescens.The percentage of degradation in soil medium are 4.32%  by Enterobacter cloacae, 23.49% by Enterobacter spand 12.19% by Pseudomonas fluorescens.A positive result indicates that bacterial growth boosters from the plant (endophyte) as well as the area of rooting (rhizosphere) have additional potential as biofertilizer, bio stimulant, bio protectant but also as bio degradator pollutants such as the herbicide glyphosate
Co-Authors Aini, Luqman Qurrata Nurul Fadilah, Nurul Tutung Hadi Astono YUNI SRI RAHAYU