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All Journal Jurnal Farmasi Galenika (Galenika Journal of Pharmacy)
Rilianawati
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology

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The Encapsulation Effect on Viability of Mesenchymal Stem Cells: Pengaruh Enkapsulasi Terhadap Viabilitas Sel Punca Mesenkimal Siburian, Marsudi; Sismindari; Rilianawati
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 7 No. 1 (2021): (March 2021)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j24428744.2021.v7.i1.15258

Abstract

Stem cells are potentially used as a regenerative therapy agent. Cell encapsulation is one of the developed methods to utilize Mesenchymal Stem Cells (MSCs) for therapy. This research aimed to study the effect of encapsulation using alginate-CaCl2 towards the viability of hAdMSCs during in vitro culture. Encapsulation of hAdMSCs with alginate-CaCl2 was done using the extrusion method. The viability of hAdMSCs was analyzed with Live/Dead Assay and MTT assay. The results indicated that cultured hAdMSCs within alginate remain alive for 7 days culture period. However, the viability was lower than the reference culture. The absorbances from MTT assay of encapsulated MSCs were lower than the conventional hAdMSCs culture. This result indicated lower metabolic activity of hAdMSCs when cultured in alginate beads.
Co-Authors Siburian, Marsudi Sismindari