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Amrianto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Materials Science & Nanotechnology Medicine & Pharmacology

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Method development of quercitrin enrichment from asthma-plant (Euphorbia hirta L.) using aromatic macroporous resin Sumail Sidik Ode Ishak; Amrianto; Diah Astari Salam; Rika Hartati
Current Research on Bioscences and Biotechnology Vol. 4 No. 1 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.4.1/2G0E50F6

Abstract

Asthma-plant contains high amount of quercitrin which make it a potential new source for flavonoids. This study aims to develop a method of quercitrin enrichment by utilizing macroporous resin, which is known to be safer, more eco-friendly, economics, and efficient. Evaluations were conducted over the performance and separation characteristics of the macroporous resin in quercitrin enrichment as well as the adsorption and desorption of quercitrin by the macroporous resin. The results showed that the adsorption process of the macroporous resin in relation to the amount of quercitrin in the extract were in accordance with the second order model, which means that the process of adsorption is affected by other compounds. Furthermore, the examination of the isotherm adsorption fit the Freundlich’s model (R2 = 0.9850) rather than the Langmuir’s one (R2 = 0.4334). In the optimal condition, the enrichment of quercitrin by using macroporous resin increased the abundance of quercitrin by nearly five times, from 3.60% of quercitrin content in the extract to 17.02% in the quercitrin-rich fraction, with recovery yield of 50.39%.
Development and validation of analysis method for sennoside B in Cassia angustifolia using UPLC-MRM/MS Amrianto; Kurnia Nastira Ningsih; Sumail Sidik Ode Ishak; Ula Aulia Fitrian; Syefira Salsabila; Diah Astari Salam
Current Research on Bioscences and Biotechnology Vol. 4 No. 2 (2023)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2023.4.2/22AMMLH7

Abstract

Cassia angustifolia is a commonly found wild plant from the family Caesalpinaceae that originates from Yemen and Hadramaut province in Southern Arabia, where it is called Arabian senna. The leaves of the plant have been used to treat a variety of ailments such as constipation, malaria, anemia, loss of appetite, and indigestion. Sennosides A and B are the major glycosides found in the leaves and pods of C. angustifolia and are important ingredients in purgative medicines. These compounds are considered as the major active components of Cassia plants and are responsible for their therapeutic activities. To assess the quality and quantity of sennosides A and B, an appropriate analytical method is required, which must be simple, accurate, precise, and widely used. The UPLC-ESI-MRM/MS method was used in this study to validate the analytical method in determining the contents of Sennoside B in senna leaves extract. The validation parameters included specificity, system suitability, linearity, sensitivity (LOD, LOQ), accuracy, and precision. The results indicated that the optimization of MRM using the direct infusion method provided good separation when eluted using liquid chromatography. The validation parameters for system suitability obtained RSD under 2%. The linearity of sennoside B showed excellent results (R2 = 0.999) in the concentration range of 0.98–62.5 µg/ml. The LOD and LOQ values of sennoside B were 0.011 µg/mL and 0.034 µg/ml, respectively. The accuracy values of sennoside B met the predetermined criteria, with RSD < 2% and % recovery of 97-102%. The quantitative analysis revealed that Cassia angustifolia extract contained 0.43 ±0.06 mg/g of sennoside B.
Liquid chromatography-mass spectrometric analysis of some bioactive compounds in commercial herbal products derived from Moringa oleifera L. Amrianto; Salsabila, Syefira; Salam, Diah Astari
Current Research on Biosciences and Biotechnology Vol. 7 No. 1 (2025)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2025.7.1/XR8PKR5P

Abstract

Moringa oleifera is widely recognized as a “miracle tree” due to its nutritional, medicinal, and environmental benefits. Its diverse phytochemical content underlies significant pharmacological effects. However, conventional HPLC methods for analyzing multi-component compounds in M. oleifera are often time-consuming and lack sensitivity, particularly for compounds present at low concentrations. To address this, we developed and validated an analytical method using UPLC-ESI-MRM/MS combined with ultrasound-assisted extraction (UAE). Method validation covered linearity, sensitivity (LOD, LOQ), accuracy, and precision, while UAE was optimized using Response Surface Methodology (RSM) with Box-Behnken Design. Seven phytochemical compounds—rutin, hyperoside, nicotiflorin, astragalin, niazirin, quercetin, and kaempferol—were quantified in various M. oleifera products including dried powder, herbal tea, extracts, and capsules. The validated method showed linearity between 1.56–500 µg/mL, LOD values of 0.036–0.094 µg/mL, LOQ values of 0.0108–0.2850 µg/mL, with accuracy and precision within ±15%. Optimal extraction was achieved at a solvent ratio of 1:30, temperature of 40 °C, and extraction time of 17.85 min. UPLC-ESI-MRM/MS enabled rapid separation and detection of all seven compounds within 15 minutes, significantly improving resolution compared to UV-based HPLC. In addition, three abundant flavonoids were confirmed as the main flavonoids present in Moringa, mainly rutin, hyperoside and nicotiflorin. On the other hand, niazirin can be considered as the specific marker for Moringa leaves. These results demonstrate that UPLC-ESI-MRM/MS, coupled with optimized UAE, provides a sensitive, rapid, and reliable approach for profiling M. oleifera phytochemicals, supporting its quality assessment and potential applications in nutraceutical and pharmaceutical industries.
Co-Authors Diah Astari Salam Kurnia Nastira Ningsih Rika Hartati Salam, Diah Astari Salsabila, Syefira Sumail Sidik Ode Ishak Sumail Sidik Ode Ishak Ula Aulia Fitrian