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All Journal Current Research on Biosciences and Biotechnology Medical Sains : Jurnal Ilmiah Kefarmasian Medical Sains : Jurnal Ilmiah Kefarmasian
Rika Hartati
Bandung Institute of Technology
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Materials Science & Nanotechnology Medicine & Pharmacology

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Isolation of bioactive compounds with tyrosinase inhibitory activity from the methanol extract of meniran herb (Phyllanthus niruri Linn.) Syefira Salsabila; Rika Hartati; Laode M.R. Al Muqarrabun; Nurinanda Prisky Qomaladewi; Ariranur Haniffadli; Andi Rifki Rosandy; Agus Chahyadi; Elfahmi
Current Research on Bioscences and Biotechnology Vol. 3 No. 2 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.3.2/GOO6ZULV

Abstract

Meniran (Phyllanthus niruri L.) is a wild plant in the Euphorbiaceae family which grows in tropical climates. The plant is known to possess antioxidant activity and is rich in phenolic compounds. Since plants with high phenolic content and high antioxidant activity are known to exhibit high tyrosinase inhibitory activity, the purpose of this study is to determine the inhibitory activity of the tyrosinase enzyme by the methanol extract of meniran (P. niruri L.) and to isolate secondary metabolites from the extract and fractions which exhibit inhibitory activity against tyrosinase. The in vitro tyrosinase inhibitory activity assay was conducted using the 96-well microplate method with kojic acid as the positive control, while the separation and purification of compounds were carried out using chromatography techniques. The methanol extract and n-hexane fraction showed tyrosinase inhibitory activity with IC50 of 11.05±0.16 and 2.57±0.08 mg/ml, respectively. Three bioactive compounds were succesfully isolated from the n-hexane fraction. Based on the UV and NMR spectra, compounds 1, 2, and 3 were identified as phyllanthin, phyltetralin, and hypophyllanthin, respectively. Furthermore, phyllanthin (1) was shown to demonstrate inhibitory activity against tyrosinase with IC50 of 0.6322 mM. In conclusion, both meniran extract and phyllanthin (1) were found to exhibit inhibitory acitivity against the tyrosinase enzyme. In the future they can be candidates for the treatment against hyperpigmentation and also as an active ingredient in skincare formulations.
Method development of quercitrin enrichment from asthma-plant (Euphorbia hirta L.) using aromatic macroporous resin Sumail Sidik Ode Ishak; Amrianto; Diah Astari Salam; Rika Hartati
Current Research on Bioscences and Biotechnology Vol. 4 No. 1 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.4.1/2G0E50F6

Abstract

Asthma-plant contains high amount of quercitrin which make it a potential new source for flavonoids. This study aims to develop a method of quercitrin enrichment by utilizing macroporous resin, which is known to be safer, more eco-friendly, economics, and efficient. Evaluations were conducted over the performance and separation characteristics of the macroporous resin in quercitrin enrichment as well as the adsorption and desorption of quercitrin by the macroporous resin. The results showed that the adsorption process of the macroporous resin in relation to the amount of quercitrin in the extract were in accordance with the second order model, which means that the process of adsorption is affected by other compounds. Furthermore, the examination of the isotherm adsorption fit the Freundlich’s model (R2 = 0.9850) rather than the Langmuir’s one (R2 = 0.4334). In the optimal condition, the enrichment of quercitrin by using macroporous resin increased the abundance of quercitrin by nearly five times, from 3.60% of quercitrin content in the extract to 17.02% in the quercitrin-rich fraction, with recovery yield of 50.39%.
ANTIMICROBIAL ACTIVITY TEST OF 96% ETHANOL EXTRACT OF FLOWERS, LEAVES, AND STEM BARK OF TIGARUN (Crateva magna DC.) AGAINST Staphylococcus aureus and Malassezia furfur Najwi Hasani; Rika Hartati; Elin Julianti
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 8 No 3 (2023)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v8i3.848

Abstract

Staphylococcus aureus and Malassezia furfur One of the microbes that can cause folliculitis is the inflammation of the skin in the hair follicles. The use of natural ingredients as antimicrobials must be developed as an alternative to overcome the resistance and side effects of chemical drugs. Tigarun (Crataeva magna DC.) is a typical plant in South Kalimantan that can be developed as a natural antimicrobial agent for the treatment of skin infections. Previous studies have reported that tigarun has antimicrobial activity, but no studies have reported its potential antimicrobial activity against folliculitis-causing microbes. Therefore, this study aimed to determine the antimicrobial activity of 96% ethanol extracts of the flowers, leaves, and stem bark of tigarun (Crataeva magna DC.) against microbes that cause folliculitis, including Staphylococcus aureus and Malassezia furfur. Extraction was performed by the maceration method using 96% ethanol. The antimicrobial activities of the extracts were tested using the agar diffusion method. Based on the results of the antimicrobial activity test using the agar diffusion method, the tigarun flower extract showed better antimicrobial activity than the leaf and tigarun bark extracts. Flower extract activity at 200 mg/mL concentrations against Staphylococcus aureus (10.65 ± 0.25) and the fungus Malassezia furfur (7.40 ± 0.40) with moderate inhibition zone ketogenic  Keywords: Folliculitis, tigarun, antimicrobial, agar diffusion
ANTIMICROBIAL ACTIVITY TEST OF 96% ETHANOL EXTRACT OF FLOWERS, LEAVES, AND STEM BARK OF TIGARUN (Crateva magna DC.) AGAINST Staphylococcus aureus and Malassezia furfur Najwi Hasani; Rika Hartati; Elin Julianti
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 8 No 3 (2023)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v8i3.848

Abstract

Staphylococcus aureus and Malassezia furfur One of the microbes that can cause folliculitis is the inflammation of the skin in the hair follicles. The use of natural ingredients as antimicrobials must be developed as an alternative to overcome the resistance and side effects of chemical drugs. Tigarun (Crataeva magna DC.) is a typical plant in South Kalimantan that can be developed as a natural antimicrobial agent for the treatment of skin infections. Previous studies have reported that tigarun has antimicrobial activity, but no studies have reported its potential antimicrobial activity against folliculitis-causing microbes. Therefore, this study aimed to determine the antimicrobial activity of 96% ethanol extracts of the flowers, leaves, and stem bark of tigarun (Crataeva magna DC.) against microbes that cause folliculitis, including Staphylococcus aureus and Malassezia furfur. Extraction was performed by the maceration method using 96% ethanol. The antimicrobial activities of the extracts were tested using the agar diffusion method. Based on the results of the antimicrobial activity test using the agar diffusion method, the tigarun flower extract showed better antimicrobial activity than the leaf and tigarun bark extracts. Flower extract activity at 200 mg/mL concentrations against Staphylococcus aureus (10.65 ± 0.25) and the fungus Malassezia furfur (7.40 ± 0.40) with moderate inhibition zone ketogenic  Keywords: Folliculitis, tigarun, antimicrobial, agar diffusion
Co-Authors Agus Chahyadi Amrianto Andi Rifki Rosandy Ariranur Haniffadli Diah Astari Salam Elfahmi Elfahmi, Elfahmi Elin Julianti Laode M.R. Al Muqarrabun Najwi Hasani Nurinanda Prisky Qomaladewi Salsabila, Syefira Sumail Sidik Ode Ishak