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All Journal Current Research on Biosciences and Biotechnology
Shoeb, Mohammad
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Materials Science & Nanotechnology Medicine & Pharmacology

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High performance liquid chromatography assessment of antibiotic residues in poultry and fish feeds in Bangladesh Islam, Rafiza; Ahammad, Raju; Islam, Md. Mazharul; Shoeb, Mohammad; Mamun, Md. Iqbal Rouf
Current Research on Biosciences and Biotechnology Vol. 6 No. 1 (2024)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2024.6.1/1AWW1JOK

Abstract

The extensive use of antibiotics in animal feed in Bangladesh raises concerns in commercial poultry and fish feeds, posing risks of antibiotic resistance in the food chain. This study aimed to critically examine feed safety and quality by assessing tetracyclines (oxytetracycline, tetracycline, and chlortetracycline) and amoxicillin in commercially produced poultry and fish feeds. Fifteen feed samples from different areas in Dhaka and Gazipur were collected and subjected to extraction and analysis with a high-performance liquid chromatography (HPLC) with photodiode array detection (PDA) method. The HPLC method was validated for linearity (R2>0.999), accuracy, and precision, limit of detection or LOD (0.72 to 1.77 µgkg-1) and limit of quantification or LOQ (1.77 to 3.69 µgkg-1), meeting European Union and Codex Alimentarius Commission standards. Matrix-matched calibration curves for each antibiotic in poultry and fish feeds exhibited excellent linearity. The sensitivity of the HPLC system was demonstrated through low LOD and LOQ. Results indicated moisture content 6.58-11.22% in poultry feeds and 6.58-11.02% in fish feeds, while ash content 4.42-12.83% in poultry feeds and 4.94-8.23% in fish feeds. Antibiotics were found to be below detection limits in all feed samples, suggesting their absence or levels below the maximum residue limits established by regulatory bodies. The study highlights the importance of monitoring antibiotics in feed to ensure food safety and mitigate antibiotic resistance risks. Further research on a larger scale is recommended to validate these findings and contribute to the development of robust regulatory frameworks for antibiotic use in animal feed production in Bangladesh.
Physicochemical properties, antimicrobial activity, and contaminants in personal care products from Bangladesh Samiha, Zeba; Joya, Sherajom Moniroh; Sultana, Saima; Islam, Md. Mazharul; Shoeb, Mohammad; Reza, Md. Shahed
Current Research on Biosciences and Biotechnology Vol. 7 No. 1 (2025)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2025.7.1/NC6ZDI7J

Abstract

Personal care products help maintain hygiene and appearance by cleansing and protecting the skin and hair, supporting overall health and barrier function. The research was aimed at evaluating the quality of different locally available skin care products. This study assessed the physicochemical properties, antimicrobial activity, and the presence of Na+ and K+ ions in various personal care products (PCPs), including face washes, shower gels, and shampoos available in Dhaka, Bangladesh. It also evaluated the total active ingredient content, saponification, acid, and iodine values, the presence of plastic microbeads, and heavy metals such as lead (Pb) and cadmium (Cd). Antibacterial susceptibility tests against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were conducted using the Agar Diffusion Method. Na+ and K+ ions were quantified using a flame photometer. Active ingredient levels, saponification, acid, and iodine values were determined through titrimetric methods. The shapes of plastic microbeads were examined with a stereo-microscope, and their composition was analyzed spectrophotometrically. Heavy metals were measured using atomic absorption spectrometry (AAS). The pH of the samples ranged from 4.62 to 10.23. Shampoos exhibited the highest antimicrobial activity, while face washes were the least effective. Na+ concentrations varied from 49.45 to 218.74 mgL-1, and K+ levels ranged from 1.3 to 125.60 mgL-1. Saponification values were in between 160.0 and 700.0 mgg-1, and acid values ranged from 0.9 to 11.0 mgg-1. Microbeads were either spherical or irregular, composed mainly of polyamide, polyethylene, and polyester. Lead concentrations varied between 0.75 and 7.54 mgL-1, with face washes containing high levels. Cadmium was below detection limits. The study highlights the need for careful selection of PCPs, considering their potential adverse effects on skin health and hygiene.
Chemical and biological activity studies of fruit of Cucumis melo L. Biswas, Tirthankar; Samiha, Zeba; Shoeb, Mohammad; Hasan, Md. Kamrul
Current Research on Biosciences and Biotechnology Vol. 7 No. 1 (2025)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2025.7.1/54J0ZGW1

Abstract

Cucumis melo L. from the family Cucurbitaceae is native to Southeast Asian countries, and very well-liked fruit due to its ginormous medicinal and nutritive value. The DCM extract of the powdered fruit sample of C. melo L. was subjected to different chromatographic techniques to isolate secondary metabolites. a-spinasterol, a-spinesteryl acetate, trilinolein and oleic acid were isolated and their structures were elucidated by comparing the data obtained from 1H NMR (400 MHz) and 13C NMR (100 MHz) spectra with reported data for corresponding compounds. The total phenolic content, total flavonoid content, total antioxidant capacity and DPPH radical scavenging activity were found to be the highest for methanolic extracts (14.25 ± 0.83 mg GAE/g, 9.2 ± 0.58 mg QE/g, 33.77 ± 1.58 mg AAE/g of dry extractives, and IC50 value of 11.13 µg/mL respectively) compared to n-hexane and DCM extracts.
Co-Authors Ahammad, Raju Biswas, Tirthankar Islam, Md. Mazharul Islam, Rafiza Joya, Sherajom Moniroh Mamun, Md. Iqbal Rouf Md. Kamrul Hasan, Md. Kamrul Reza, Md. Shahed Samiha, Zeba Sultana, Saima