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Mikdariawan, Adha
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science

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ISOLATION OF ENDOPHYTIC BACTERIA FROM OKRA FRUIT (Abelmoschus esculentus L. Moench) Mikdariawan, Adha; Astuti, Winni; Marliana, Eva
JURNAL ATOMIK Vol 6 No 1 (2021)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Endophytic bacteria is a bacteria that live in plant tissues without disrupt the plant's life cycle. This research aims to isolate endophytic bacteria from okra fruit (Abelmoschus esculentus L. Moench). Before isolation, okra fruit was sterilized by the outer surface with aquadest, 5.25% NaClO and 95% ethanol. The okra fruit sample is then cut and the inside of the okra fruit is cultured in a nutrient agar (NA). The results of this test indicate that there are endophytic bacteria that grow characterized by the presence of turbid areas around the sample. The results of isolation of endophytic bacteria using the method of stocking cup were obtained by 28 isolates of endophytic bacteria.
Co-Authors Eva Marliana, Eva Winni Astuti, Winni