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Isolasi dan Uji Potensi Bakteri Pelarut Fosfat dari Rhizosfer Tanaman Kakao (Theobroma cacao) Sutaryo, Setya Puji Handayani; Achmad Himawan; Kristalisasi, Elizabeth Nanik
AGROISTA : Jurnal Agroteknologi Vol. 8 No. 1 (2024): MEI
Publisher : Program Studi Agroteknologi INSTIPER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55180/agi.v8i1.1004

Abstract

Phosphate solubilizing bacteria are important to caccao plants for phosphor ion release, so that it is not attached by Fe or Al ion, and it use by plants. These research purposes  are to isolation of bacteria that is predicted to have potential as phosphate solubilizing bacteria and it’s identification. Research was conducted at Laboratorium Pusat INSTIPER Yogyakarta in March – May 2023. Research used descriptive method, survey at different caccao orchard, sterilization of research tools and materials, sampling of rhizosphere soil of caccao plants, isolation and bacteria identification. Macroscopy observation results were obtained 4 isolates that were showed transparent zones in Pikovskaya’s media and it’s potential as phosphate solubilizing bacteria. Microscopy observation results were obtained phosphate solubilizing bacteria from every location of sampling that are location 1 at Nglanggeran Kulon hamlet (isolate K1P3U5), location 2 at Gunung Buthak hamlet (isolate K2P2U1) and location 3 at Nglanggeran Wetan hamlet (isolate K3P4U5 and K3P2U2), Nglanggeran Village, Patuk Subdistrict, Gunung Kidul Regency. Phosphate solubilizing bacteria that were obtained are Genus Acetobacter from K1P3U5 and K3P4U5 isolates, and  Escherichia from K2P2U1 isolate. From K3P2U2 isolate was not  identification yet.
Keragaman Penyakit pada Bibit Pre Nursery dan Main Nursery Kelapa Sawit di PT. Socfindo Kebun Bangun Bandar, Kec. Dolok Masihul, Kab. Serdang Berdagai Sumatera Utara Suryansyah, Mhd. Rizky; Himawan, Achmad; Kristalisasi, Elizabeth Nanik
AGROISTA : Jurnal Agroteknologi Vol. 8 No. 2 (2024): NOVEMBER
Publisher : Program Studi Agroteknologi INSTIPER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55180/agi.v8i2.1502

Abstract

Oil palm productivity is influenced by the availability of quality seeds. One important factor includes making seeds or nurseries. Single stage and double stage are two categories included in the provision of seeds based on type. To evaluate the frequency and severity of disease attacks, this study aims to identify various diseases that attack oil palms in pre nursery and main nursery. From July to August 2024, research was conducted at the Bangun Bandar plantation, North Sumatra Province, in an oil palm nursery area managed by PT. Socfindo. Purposive sampling was used as a survey method. Abiotic diseases (stunted, curly, grass like leaves, curly, and not planted properly) and biotic diseases (leaf spots) are often found in prenurseries. On the other hand, abiotic diseases (stunt, wrinkled leaves, curled leaves, twisted leaves, incompletely opened leaves, twisted leaves and misplanting), biotic diseases (leaf spots) are also often found in the main nursery. In the pre-nursery, leaf spot was the largest percentage of disease attacks (25.50%), but in the main nursery it was 28.55%. At pre nursery, leaf spot disease (9.50%) and the main nursery (11.50%) had the highest level of disease attack severity, both of which were classified as light damage.
ANTAGONIST TEST OF TRICHODERMA HARZIANUM AGAINST GANODERMA BONINENSE CAUSES OF OIL PALM BASAL STEM ROT DISEASE Kusmawanto, Arif; Himawan, Achmad; Kristalisasi, Elizabeth Nanik
Journal of Agriculture Vol. 1 No. 03 (2022): Research Articles, November 2022
Publisher : ITScience (Information Technology and Science)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47709/joa.v1i03.1938

Abstract

This study aims to determine the type of Trichoderma fungus that can inhibit the growth of G. boninense and determine the percentage of inhibition of Trichoderma on the growth and development of G. boninense. This research was conducted at the Central Laboratory of the STIPER Agricultural Institute Yogyakarta in February-March 2022. G. boninense samples were taken from three different plantation locations belonging to residents of Aek Paing Atas Village and Aek Paing Bawah Satu Village, Kec. Rantau Utara, Kab. Labuhan Batu and Aek Janji Village, Kec. Rantau Selatan, Kab. Labuhan Batu, North Sumatra. Trichoderma samples were taken from Naga Soppah Village, Naga Harjo Village and Bahung Kahean Village. The research method used is descriptive by identifying antagonistic (Trichoderma) and pathogenic (G. boninense) fungi. Data on the percentage of inhibition of fungi were analyzed quantitatively. Fungi macroscopic and microscopic characterizations were analyzed qualitatively. The results showed that the Trichoderma fungus that could inhibit the growth of G. boninense was T. harzianum. The highest percentage of inhibition power of T. harzianum (Naga Soppah Village) against G. boninense (Aek Paing Bawah Satu village) was 30%.
The effect of Concentration and Application Method of Potato Starch Edible Coating on the Quality of Tomatoes Ramadani, Rifani; Syah, Ryan Firman; Kristalisasi, Elizabeth Nanik
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8501

Abstract

Tomatoes are a perishable commodity meaning they are prone to spoilage and physiological damage after harvest can affect their shelf life, solution to address this issue is by using coating. This study aims to determine how the interaction between concentration and application method of potato starch edible coating affects the results and to find the best concentration and application method of the potato starch edible coating. The research was conducted at the central laboratory of the Instiper campus in Maguwoharjo, Sleman District, Yogyakarta. A Completely Randomized Design (CRD) with two factors was used in this study. The first factor is the concentration of the edible coating solution, which consists of 5 levels (control, 2.5%, 5%, 7.5%, and 10%). The second factor is the application method of the potato starch edible coating, which consists of 3 levels (dipping, spraying, and brushing), each with 3 replications. The data obtained were then analyzed using Analysis of Variance (ANOVA) at a 5% significance level. If significant differences were found, further tests were performed using Duncan’s Multiple Range Test (DMRT) at a 5% significance level. The results showed that there is an interaction between the concentration and application method of the potato starch edible coating on the vitamin C content and texture of the tomatoes. Tomatoes coated with 5% potato starch edible coating showed the best results in terms of vitamin C content and texture, and the application method of the edible coating produced similar results when compared to dipping in terms of tomato color.
Identifikasi Jamur pada Akar Tanaman Karet yang Berasal dari KP 2 (SEAT) Ungaran Irfani, Hidayat; Himawan, Achmad; Kristalisasi, Elizabeth Nanik
AGROISTA : Jurnal Agroteknologi Vol. 9 No. 2 (2025): NOVEMBER
Publisher : Program Studi Agroteknologi INSTIPER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55180/agi.v9i2.1653

Abstract

Fungi can grow and live in plant tissue. Endophytic fungi can provide benefits to plants in the form of increased resistance to disease attacks and can stimulate growth. This research aims to isolate and identify fungi in the roots of rubber plants in KP2 (SEAT) Ungaran. Sampling was taken at the KP2 (SEAT) Ungaran rubber plantation, followed by isolation and identification at the Laboratory of the Institute of Agriculture STIPER Yogyakarta. The research started from April to June 2024. Sampling used a diagonal method and the fungal identification method used a descriptive method. The results of isolation and identification obtained 13 isolates identified as the genus Colletotrichum sp. (B1P5U1 and B3P2U4), Fusarium sp. (B1P5U2, B1P5U3, B2P2U3 and B3P3U3), Geotrichum sp. (B2P5U1), Paecilomyces sp. (B3P1U5), Penicillium sp. (B1P1U3, B1P4U5, and B3P2U2), Rhizoctonia sp. (B2P4U3), Rhizopus sp. (B1P1U4), and 2 unknown isolates (B2P4U2 and B2P5U2).