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All Journal EnviroScienteae Jurnal Pengelolaan Laboratorium Pendidikan
Fariani, Rini
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Education Environmental Science

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PERBANDINGAN PROFIL FITOKIMIA DAN GCMS MENGGUNAKAN EKSTRAKSI MASERASI DAN SOKHLETASI SERTA PROFIL HISTOKIMIA DAUN SUNGKAI (P. canescens Jack.) Jaya, Fahriza Kusuma; Fariani, Rini; Ain, Nor
EnviroScienteae Vol 20, No 4 (2024): ENVIROSCIENTEAE VOLUME 20 NOMOR 4, NOVEMBER 2024
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/es.v20i4.21055

Abstract

Sungkai leaf (P. canescens Jack.) is a native plant of Indonesia that has long been used in traditional medicine as a mouthwash and minor wounds. Sungkai leaves P. canescens Jack. are believed by the community to have many health benefits. Several studies have shown that sungkai leaves (P. canescens Jack.) contain active compounds. As for the extraction process, it is known that the content of active compounds obtained in the sungkai leaf extract is strongly influenced by the selection of solvents used and the extraction method carried out. In previous studies, total flavonoid content has been obtained using maceration and soxhletation methods in sungkai leaf extracts, where flavonoids are one of the profiles of secondary metabolite compounds possessed by sungkai leaves. In the next research, the profile of sungkai leaves will be determined more thoroughly through phytochemical screening and GCMS analysis of the extraction results using maceration and soxhletation methods. The histochemical profile of sungkai leaves will also be determined. Histochemical test results show that flavonoid compounds are evenly distributed in all tissues of sungkai leaves, besides that some tissues in sungkai leaves show positive results for the presence of alkaloid, steroid, terpenoid, tannin, phenol and saponin compounds.The results of phytochemical screening showed that sungkai leaves positively contained secondary metabolite compounds in the form of alkaloids, flavanoids, steroids, terpenoids, tannins, phenolics, saponins while the results of GCMS analysis for the maceration process obtained metabolite compounds in the form of terpenoids, alkaloids, and phenolics, while for the sokletation process obtained metabolite compounds in the form of flavonoids, terpenoids, and phenolics.
Pengaruh Cryprotectant Agents (Gliserol) pada Media Preservasi Terhadap Daya Simpan Pseudomonas aeruginosa FNCC 0063 Rasyidah, Rasyidah; Fariani, Rini
Jurnal Pengelolaan Laboratorium Pendidikan Vol.8, No.1, Januari 2026
Publisher : UPT Laboratorium Terpadu, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jplp.8.1.47-54

Abstract

Preservation using glycerol as cryoprotectant agents is expected to be an alternative in maintaining bacterial viability, so that when they are to be used again, the bacteria can grow. The objective of this study was to determine the effect of glycerol concentrations of 10%, 30%, and 50% in the preservation medium and preservation time on the viability of Pseudomonas aeruginosa FNCC 0063 during storage, as well as to determine the effective glycerol concentration and time. Preservation of Pseudomonas aeruginosa FNCC 0063 in the form of bacterial suspensions and pellets was conducted for 30–150 days at a freezer temperature of –40°C. Viability tests were performed from day 30 to 150 using the Plate Count method with Nutrient Agar medium. Two-way ANOVA test of pellet preservation from cell viability data,  significant value 0.000 < 0.05, meaning that viability or the number of living cells is influenced by time and glycerol concentration. Preservation time and glycerol concentration based on the Tukey test results showed the highest average value on day 60 and a concentration of 50%, which was 9.4 x 10⁵ CFU/ml. Suspension preservation: the two-way ANOVA test results showed that preservation time had an effect, but glycerol concentration did not significantly affect cell viability. Tukey test showed that the preservation results on day 120 were significantly different from those at 30–90 days. As for concentration treatment, there were no significant differences between 10%, 30%, and 50%, but the 50% concentration had relatively stable and high viability compared to 10% and 30%, at 6.7 × 10⁵ CFU/ml.
Co-Authors Ain, Nor Jaya, Fahriza Kusuma Rasyidah Rasyidah