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Comparative Analysis of Maceration and Soxhlation Extraction for The Total Flavonoid Content of Sungkai Leaves (Peronema canescens Jack.) Jaya, Fahriza Kusuma
Jurnal Sains dan Terapan Kimia Vol 18, No 1 (2024)
Publisher : Program Studi Kimia, Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jstk.v18i1.17638

Abstract

The sungkai leaf (P. canescens Jack.) is an indigenous plant of Indonesia that has been utilized as a mouthwash and for treating minor wounds in traditional medicine. This study aimed to determine the total flavonoid content of P. canescens Jack leaf extract using maceration and soxhlation extraction methods. The total flavonoid content in the leaf extract of P. canescens Jack was determined using a UV-Vis spectrophotometer and a colorimetric technique (AlCl3) at a wavelength of 412 nm. The results were reported as the total flavonoid content in quercetin equivalent (EQ). The maceration step yielded 7.30%, whereas the soxhlation process yielded 15.34%. The maceration method yielded a total flavonoid content of 81.19 mgEQ/gram extract, whereas the soxhlation process yielded a flavonoid content of 69.068 mgEQ/gram extract.
PERBANDINGAN PROFIL FITOKIMIA DAN GCMS MENGGUNAKAN EKSTRAKSI MASERASI DAN SOKHLETASI SERTA PROFIL HISTOKIMIA DAUN SUNGKAI (P. canescens Jack.) Jaya, Fahriza Kusuma; Fariani, Rini; Ain, Nor
EnviroScienteae Vol 20, No 4 (2024): ENVIROSCIENTEAE VOLUME 20 NOMOR 4, NOVEMBER 2024
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/es.v20i4.21055

Abstract

Sungkai leaf (P. canescens Jack.) is a native plant of Indonesia that has long been used in traditional medicine as a mouthwash and minor wounds. Sungkai leaves P. canescens Jack. are believed by the community to have many health benefits. Several studies have shown that sungkai leaves (P. canescens Jack.) contain active compounds. As for the extraction process, it is known that the content of active compounds obtained in the sungkai leaf extract is strongly influenced by the selection of solvents used and the extraction method carried out. In previous studies, total flavonoid content has been obtained using maceration and soxhletation methods in sungkai leaf extracts, where flavonoids are one of the profiles of secondary metabolite compounds possessed by sungkai leaves. In the next research, the profile of sungkai leaves will be determined more thoroughly through phytochemical screening and GCMS analysis of the extraction results using maceration and soxhletation methods. The histochemical profile of sungkai leaves will also be determined. Histochemical test results show that flavonoid compounds are evenly distributed in all tissues of sungkai leaves, besides that some tissues in sungkai leaves show positive results for the presence of alkaloid, steroid, terpenoid, tannin, phenol and saponin compounds.The results of phytochemical screening showed that sungkai leaves positively contained secondary metabolite compounds in the form of alkaloids, flavanoids, steroids, terpenoids, tannins, phenolics, saponins while the results of GCMS analysis for the maceration process obtained metabolite compounds in the form of terpenoids, alkaloids, and phenolics, while for the sokletation process obtained metabolite compounds in the form of flavonoids, terpenoids, and phenolics.