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Effect of Giving Turmeric Flour (Curcuma domestica) on Differential Leukocytes, Antibody Titers of Avian Influenza and Newcastle Disease Super Native Chickens Nur, Adyatma Muhammad; Purwanti, Sri; Rahardja, Djoni Prawira; Mutisari, Dewi
ANIMAL PRODUCTION Vol. 25 No. 2 (2023)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2023.25.2.196

Abstract

In order to raise super-native chickens without the usage of antibiotics, herbal plants with a phytobiotic function may be used as feed additions. This study aims to examine the effect of turmeric flour (Curcuma domestica) given through drinking water on leukocyte differentials, AI antibody titers, and ND antibody titers as indicators of immunity status in super-native chickens. Five treatments and four tests, each with five super native chicks, were used in the experiment, which was carried out utilizing an experimental methodology. The treatment (P) given included P0 as a negative control (drinking water without treatment), P1 as a positive control (drinking water + 0.1 g/L PromuneC®), P2 (drinking water + 1 g/L turmeric flour), P3 (drinking water + 3 g/L turmeric flour) and P4 (drinking water + 5 g/L turmeric flour). All the data gathered for this study were evaluated using Analysis of Variance, and Duncan's test using SPSS version 25 was required if there were any significant differences. The 56-day-old AI and ND antibody titer reached a protective level where the best dose for AI antibody titer increase was 5 g/L of drinking water with AI log2 9.5 of titer and the best dose for ND antibody titer increase was 3 g/L of drinking water with ND log2 7.5 of titer. According to statistical analysis, the addition of turmeric powder to drinking water had no discernible effects on the mean of lymphocytes, monocytes, or eosinophils but had a significant impact (P0.05) on the mean of basophils. The treatment also had a noticeable effect on AI antibody titers (P<0.05) but had no noticeable effect on ND antibody titers. In conclusion, giving turmeric powder (Curcuma domestica) in drinking water is useful as an immunomodulator by maintaining normal levels of differential leukocytes, and increasing protection against AI and ND super-native chicken so it can be used as a natural feed additive.
Pathogenicity and Classification of Influenza A Virus in poultry isolates from Sulawesi in 2018 Mutisari, Dewi; Muflihanah; Ferra Hendrawati; Lestari; Hendra Wibawa
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 7, No 2, JULY 2023
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v7i2.22039

Abstract

Avian influenza (AI) is a viral infection caused by the Influenza virus type A. Infection with the Avian Influenza Virus (AIV) has resulted in major financial losses in the cattle industry as well as substantial public health consequences. Indonesia has been dealing with an HPAI H5N1 outbreak since 2003. Despite the implementation of many prevention and control measures, the AIV disease continues to spread. Three Sulawesi isolates were submitted to whole-genome sequencing (WGS) in 2018 using the Illumina next generation sequencing (NGS) technology. The BioEdit 7 molecular analysis application was used to do multiple alignments and amino acid prediction. The Influenza Research Database's Highly Pathogenic H5N1 Clade Classification Tool (https://www.fludb.org) was used for clade analysis. One of the virus's pathogenicity is the amino acid cleavage site in the hemagglutinin (HA) gene. We concluded that the PQRERRRK-GLF amino acid motif was present in the cleavage site of the HA gene in avian AIV isolates from Sulawesi in 2018. This indicates that the AIV virus isolates are pathogenic and highly virulent avian influenza viruses (HPAI). Clade analysis revealed that the AIV isolates were from the H5N1 virus clade 2.3.2.1c.