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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Lahan Suboptimal
Amy Estiati
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Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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Penapisan Beberapa Padi Lokal dari Pulau Enggano Terhadap Ketahanan Salinitas Dwi Astuti; Ade Nena Nurhasanah; Satya Nugroho; Amy Estiati
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol. 6 No. 2 (2017): JLSO
Publisher : Research Center for Sub-optimal Lands (PUR-PLSO), Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (575.544 KB) | DOI: 10.33230/JLSO.6.2.2017.282

Abstract

Astuti et al, 2017. Screening of Several Local Rice from Enggano Island to Salinity Tolerance. JLSO 6(2):134-141. Enggano Island, Bengkulu Province, has a lot of local rice germplasm that has been cultivated by people in Enggano Island that is more than 30% as farmer. Identification of local rice from Enggano Island against biotic and abiotic stresses, especially salinity stress is necessary. The identification can be used, among other things, as the information of the local rice planting that related to the rising sea level in Enggano Island which impact on the condition and the area of rice cultivation land, and as a source of finding for resistance genes against salinity stress for improvement of those local rice varieties. Aim of this study is preliminary screening of some local rice from Enggano Island to salinity stress in seed and germination stages. The analysis used Factorial Randomized Design, two factors, the first factor  is salt concentration and and the second is local variety. The salt concentrations used were 0 millimolar NaCl, 250 millimolar NaCl, NaCl 275 millimolar and NaCl 300 millimolar and the rice sample used were 11 local rice of Enggano Island, NiponBare varieties as control of rice plants, Pokali as resistant control and IR29  varieties as susceptible controls. Rice seeds that used in this test are seed in seed stage and germination stage of 10-14 days old. This preliminary screening shows three local genotype of Enggano Island that tolerance to Salinity.
Konfirmasi Toleransi Galur-galur Padi terhadap Cekaman Kekeringan secara Molekuler Wening, Rina Hapsari; Willy Bayuardi Suwarno; Bambang Sapta Purwoko; Indrastuti Apri Rumanti; Amy Estiati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 49 No. 2 (2021): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (56.444 KB) | DOI: 10.24831/jai.v49i2.36565

Abstract

Seleksi secara fenotipik, terutama terhadap cekaman abiotik, seringkali sulit dilakukan. Kegiatan seleksi secara molekuler diperlukan untuk mengidentifikasi hingga taraf molekuler bahwa suatu sifat toleran terbukti secara genotipik. Tujuan dari penelitian ini ialah untuk mengkonfirmasi sifat toleransi kekeringan galur-galur padi secara molekuler menggunakan marka SSR RM164, RM228, RM248, dan RM328. Penelitian dilaksanakan di Laboratorium Bioteknologi LIPI, Cibinong pada bulan November hingga Desember 2017, menggunakan teknik polymerase chain reaction (PCR). Hasil penelitian menunjukkan bahwa marka RM228 terkait dengan karakter skor menggulung, panjang malai, tinggi tanaman dan umur berbunga, sedangkan RM328 terkait dengan panjang malai, jumlah anakan, jumlah gabah isi per malai, dan produktivitas. Marka tersebut diduga dapat digunakan untuk seleksi toleransi kekeringan. Tujuh dari sepuluh galur yang terindikasi toleran terhadap cekaman kekeringan fase generatif berdasarkan skrining secara artifisial terkonfirmasi secara molekuler berada dalam satu kelompok dengan varietas pembanding toleran ‘Limboto’. Ketujuh galur tersebut ialah B13983E-KA-12-2, B13507E-MR-19, B14366E-KY-50, BP20452e-PWK-0-SKI-1-1, BP20452e-PWK-0-SKI-2-4, BP20452e-PWK-0-SKI-3-3, dan BP29790d-PWK-3-SKI-1-5. Kata kunci: marka, toleransi kekeringan, polymerase chain reaction
Co-Authors Ade Nena Nurhasanah Bambang Sapta Purwoko Dwi ASTUTI Indrastuti Apri Rumanti Rina Hapsari Wening, Rina Hapsari Satya Nugroho Willy Bayuardi Suwarno