Rina Hapsari Wening, Rina Hapsari
Balai Besar Penelitian Tanaman Padi Jl. Raya 9 Sukamandi, Subang, Jawa Barat

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Genotyping of rice (Oryza sativa L.) plants according to their root distribution pattern and their tolerance to drought Novitasari, Adin; Rumanti, Indrastuti Apri; Wening, Rina Hapsari; Damanhuri, Damanhuri
Jurnal Ilmu Pertanian Vol 8, No 3 (2023): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.84677

Abstract

Drought condition is one of the major problems in producing rice in Indonesia. In plant breeding, selection is the main step to create superior varieties. An indicator of drought stress-tolerant rice varieties is the root distribution pattern because it describes the behavior of the roots in searching for water for photosynthesis and metabolism processes. One of the morphological traits related to drought tolerance in rice is root growth and development. This study aimed to determine the morphological and anatomical characteristics of drought-tolerant rice roots and identify drought-tolerant genotypes. The experiments were arranged in a factorial randomized block design with four replications. The first factor was genotype, consisting of ten genotypes. The second factor was drought stress, consisting of two environments without drought and with drought stress. Drought was given only in the vegetative phase, i.e., 1–14 days after planting. WINDEX analysis was performed to determine rice drought tolerance and identify drought-tolerant genotypes. The results showed that three out of the ten tested genotypes had higher WINDEX values, namely BP30411f (7.62), B13983-KA-6-3 (7.99), and BP29790d-PWK-2-SKI-1-3 (9.25). Based on the root distribution pattern, plants with longer primary root lengths, more seminal roots, longer seminal root lengths, and high root angles were predicted to be drought tolerant characteristics. Selection of these characters could be used in future rice breeding programs to obtain plants with superior genotypes.
Keragaan Pertumbuhan dan Hasil Sepuluh Genotipe Tanaman Padi (Oryza Sativa L.) Novitasari, Adin; A. Rumanti, Indrastuti; Wening, Rina Hapsari; Damanhuri, Damanhuri
Produksi Tanaman Vol. 7 No. 4 (2019)
Publisher : Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Padi (Oryza sativa L.) adalah tanaman pangan yang dimanfaatkan bijinya. Permintaan beras semakin meningkat setiap tahunnya akan tetapi produktivitas semakin menurun. Peningkatan produk-tivitas beras dapat dilakukan dengan ekstensifikasi yaitu pemanfaatan lahan-lahan suboptimal seperti lahan rawa lebak dan tadah hujan.Ketersediaan air pada lahan tersebut tidak menentu misalkan terjadi kekeringan sehingga diperlukan varietas yang memiliki daya hasil tinggi.Tujuan penelitian ini adalah untuk mengetahui potensi hasil dari sepuluh genotipe padi.Penelitian dilaksanakan mulai Desember 2017 sampai dengan April 2018 di Kebun Percobaan Balai Besar Penelitian Tanaman Padi (BB Padi), Subang, Jawa Barat.Metode yang digunakan adalah Rancangan Acak Kelompok (RAK) dengan 10 perlakuan genotipe dan diulang tiga kali. Analisis data yang digunakan pada percobaan ini adalah analisis ragam (Uji F) 5% dan apabila hasil menunjukkan beda nyata dilakukan uji lanjut menggunakan Uji Beda Nyata Terkecil (BNT) 5% dan analisis korelasi. Hasil penelitian menunjukkan bahwa B13983E-KA-6-3 memiliki hasil tertinggi yaitu 8.06 ton ha-1 meskipun tidak berbeda nyata dengan Inpari 38 (6,75 ton ha-1). Karakter pertumbuhan vegetatif yang berkorelasi positif dengan hasil yaitu tinggi tanaman (r=0.08) danjumlah anakan (r=0.49). Karakter pertumbuhan generatif yang berkorelasi positif dengan hasil adalah umur berbunga (r=0.44) dan umur masak(r=0.15). Karakter komponen hasil yang berkorelasi positif dengan hasil adalah presentase gabah isi (r=0.10), bobot 1000 butir (r=0.11) dan bobot gabah per rumpun (r=0.17).
Seleksi Simultan Karakter Daun Mengering dan Produktivitas pada Galur-galur Padi Wening, Rina Hapsari; Purwoko, Bambang Sapta; Suwarno, Willy Bayuardi; Rumanti, Indrastuti Apri; Khumaida, Nurul
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 3 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (569.772 KB) | DOI: 10.24831/jai.v47i3.26076

Abstract

Drought is an important constraint for rice production in rainfed lowland and shallow freshwater swamp. The area often experiences drought stress at the generative stage of the plants. This study aimed at selecting adaptive lines to terminal drought and formulating a multiple regression model to estimate the productivity under drought stress conditions at the generative stage. The experiment was conducted in a greenhouse of the Indonesian Center for Rice Research, Subang, West Java, using an augmented design with five blocks. The genetic material used was ninety-nine lines and four checks varieties, namely Inpari 30, Limboto, Salumpikit, and IR 20. The model was formulated using stepwise regression analysis. Based on this study, ten lines were adapted to drought stress at the generative stage, namely B13983E-KA-12-2, B13926E-KA-13, B13507E-MR-19, B14366E-KY-50, B14366E-KY-37, IR86384- 46-3-1-B, BP20452e-PWK-0-SKI-1-1, BP20452e-PWK-0-SKI-2-4, BP20452e-PWK-0-SKI-3-3, and BP29790d-PWK-3 -SKI-1-5. The B13507E-MR-19 had the highest productivity (4.02 ton ha-1) under drought stress conditions. Yield under drought stress in the greenhouse could be predicted using a linear regression model involving plant height at early vegetative stage, plant height up to the panicle, tiller number at early vegetative stage, tiller number at late vegetative stage, tiller number at flowering, heading time, number of filled grain, and panicle exsertion length. This model was able to explain 75.92% of yield variation. Potential rice lines and the regression model obtained are expected to contribute to the development of rice varieties adaptive to drought. Keywords: drought tolerant, freshwater swamp, rainfed, regression model
Konfirmasi Toleransi Galur-galur Padi terhadap Cekaman Kekeringan secara Molekuler Wening, Rina Hapsari; Willy Bayuardi Suwarno; Bambang Sapta Purwoko; Indrastuti Apri Rumanti; Amy Estiati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 49 No. 2 (2021): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (56.444 KB) | DOI: 10.24831/jai.v49i2.36565

Abstract

Seleksi secara fenotipik, terutama terhadap cekaman abiotik, seringkali sulit dilakukan. Kegiatan seleksi secara molekuler diperlukan untuk mengidentifikasi hingga taraf molekuler bahwa suatu sifat toleran terbukti secara genotipik. Tujuan dari penelitian ini ialah untuk mengkonfirmasi sifat toleransi kekeringan galur-galur padi secara molekuler menggunakan marka SSR RM164, RM228, RM248, dan RM328. Penelitian dilaksanakan di Laboratorium Bioteknologi LIPI, Cibinong pada bulan November hingga Desember 2017, menggunakan teknik polymerase chain reaction (PCR). Hasil penelitian menunjukkan bahwa marka RM228 terkait dengan karakter skor menggulung, panjang malai, tinggi tanaman dan umur berbunga, sedangkan RM328 terkait dengan panjang malai, jumlah anakan, jumlah gabah isi per malai, dan produktivitas. Marka tersebut diduga dapat digunakan untuk seleksi toleransi kekeringan. Tujuh dari sepuluh galur yang terindikasi toleran terhadap cekaman kekeringan fase generatif berdasarkan skrining secara artifisial terkonfirmasi secara molekuler berada dalam satu kelompok dengan varietas pembanding toleran ‘Limboto’. Ketujuh galur tersebut ialah B13983E-KA-12-2, B13507E-MR-19, B14366E-KY-50, BP20452e-PWK-0-SKI-1-1, BP20452e-PWK-0-SKI-2-4, BP20452e-PWK-0-SKI-3-3, dan BP29790d-PWK-3-SKI-1-5. Kata kunci: marka, toleransi kekeringan, polymerase chain reaction