<![CDATA[This study was aimed to detect KLF14 gene polymorphism in Iraqi type 2 diabetes mellitus patients (T2DM)to found the correlation between the SNP (rs972283) polymorphism in KLF14 gene and lipid metabolismand impact on the incidence of type 2 diabetes mellitus (T2DM). The result show high Significant differencewas observed in FBS level (P<0.001) in patient group (172.9 ± 79.73) and in control (81.16 ± 7.18),).Cholesterol, LDL-Cholesterol and VLDL-Cholesterol mean level value in diabetic patients was significantlyhigher than those of control group (p<0.0001), serum HDL-Cholesterol mean value was significantlydifference (P<0.05) in serum mean level of HDL between T2DM patients and healthy controls. Real timePCR (HRM) RT-PCR were used to detect SNP (rs972283) in KLR14 gene (G>A) by using specific primers,as a related with SNP (rs972283) G>A in KLF14 gene, genotypes and alleles frequencies, odds ratios,95% confidence intervals and P values for the KLF14 gene In this study, statistical analyses of genotypicfrequencies for the KLF14 (rs972283) revealed significant difference between T2DM patients and controlsin the examined population. GG genotype was significantly (P = 0.0006) more frequent in the patient group.The observed G/G, A/G, and A/A genotype frequencies were 50%, 42%, and 8%, respectively.The A (wildtype)and G (variant) allele frequencies were 29% and 71% , respectively in patient group while the observedG/G, A/G, and A/A genotype frequencies were 22%, 48%, and 30, respectivelyand the A (wild-type) , G(variant) allele frequencies were 54% and 46%, respectively in control group (P <0.0003). GG genotype wassignificantly (P = 0.0006) more frequent in the patient group, Conclusion: There was a relationship betweenpolymorphism of KLF14 gene SNP (rs972283) and the incidence of T2DM in sample of Iraqi patients.]]>
