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All Journal PROSIDING SEMINAR NASIONAL Prosiding Seminar Nasional Unimus
Ragil Saptaningtyas
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Journal : PROSIDING SEMINAR NASIONAL

 1 
KARAKTERISASI MOLEKULER PROTEIN FLAGELLIN Salmonella typhi ISOLAT JAWA TENGAH DAN YOGYAKARTA Sri Darmawati; Budi Santosa; Ragil Saptaningtyas
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Bidang MIPA dan Kesehatan The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

The purpose of this study is to molecularly characterize 7 strains of flagellin proteins of Salmonella typhi isolates taken from Central Java (5 strains from Semarang, 1 strain from Salatiga, 1 strain from Magelang) and 2 isolate strains from Yogyakarta (1 strain from Doctor Sarjito Hospital, and 1 strain from Bethesda Hospital). Flagellin gene (fliC) amplification is conducted using PCR (primer LPW 1856 and LPW 1857). Flagellin proteins resulted from Alexan method (2009) is then modified while flagellin protein profiles are obtained with SDS-PAGE method. The results show that the size of flagellin gene (fliC) of S. typhi Isolates from Salatiga and the 8 other strains is respectively 1262bp and 1500bp. Flagellin proteins which are composed of 2-6 protein sub-units consist of 1-2 major proteins and 1-4 minor proteins with the sizes of 16-116 kDa.Keywords: Salmonella typhi, flagellin, Molecular Characterizations
HAEMAGGLUTINATION ACTIVITY OF Salmonella typhi FLAGELLIN PROTEIN BASED ON ABO BLOOD GROUP Ragil Saptaningtyas; Sri Darmawati; Sri Sinto Dewi
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Student Paper Presentation The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Salmonella typhi is a negative basil Gram bacterium that causes typhoid fever. Flagel of S. typhi contain proteins, as locomotor and it can help bacteria to attach on host cells. Haemagglutinin protein is protein that can agglutinate erythrocytes. The goal of this research is to analize S. typhi flagellin proteins haemagglutination activity Based on ABO blood group. Flagellin proteins isolation method of modified Alexan method (2009) and haemagglutination test method of Finkeltein and Hanne method (1982). Haemagglutination result of ABO blood type show that SLT-1 S. typhi flagellin proteins can’t agglutinate human erythrocytes A, B, and O, but it can agglutinate human erythrocytes until 8 times dilution of AB blood type from 50 μg/μl concentration in 50 μl. BA 07.4 S. typhi flagellin proteins can agglutinate human erythrocytes of A blood type until 16 times dilution, 8 times dilution of AB blood type, 4 times dilution of O blood type, and it can’t agglutinate human erythrocytes B blood type.Keywords: Salmonella typhi, Flagellin Proteins, Haemagglutination, Erythrocytes of ABO System.
Molecular Characterization And Hemagglutination Activities of Flagellin Protein of Salmonella typhi Sri Darmawati; Budi Santosa; Muhammad Evy Prastiyanto; Ragil Saptaningtyas
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2016: Proceeding of International Seminar on Education Technology (ISET) 2016
Publisher : Universitas Muhammadiyah Semarang

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Abstract. The purposes of this research are for molecular characterization and hemagglutination activity test of flagellinprotein of Salmonella typhi. The research samples consist of 7 strains of S. typhi isolates from Central Java (5 strains from Semarang city, 1 strain from Salatiga and 1 strain from Magelang) and 2 strains of S. typhi from Yogyakarta (Doctor Sardjito Hospital and Bethesda Hospital). The undertaking procedures are: 1) PCR and sequencing of fliC genes using primer LPW 1856 and LPW 1857.2) Isolation and separation of flagellin protein using SDS-PAGE. 3) Hemagglutination Activity Test upon human erythrocytes of blood group A, B, AB and O.The results show that 8 strains of S. typhi have a fliC gene size of 1452 to 1488 bp including serovar H1-d, and 1 strain with the size of 1267 bp including serovar H1-J. Flagella protein resulted from SDS-PAGE protein consists of 1-2 major proteins and 1-3 minor proteins with a molecular weight of 16-116 kDa. The results of hemagglutination activity test of flagellin protein show that there are 3 strains of S. typhi (MG-1, SA02.2 and BET) which are able to agglutinate human erythrocytes of bloodgroup A, B, AB and O (2-64HA), 6 other strains show various hemagglutination activities varied
SAMPLING MIKROBIOLOGI LIMBAH BIOMEDIS RUMAH SAKIT DI KOTA SEMARANG JAWA TENGAH Stalis Norma Ethica; Sakti Imam Muchlissin; Ragil Saptaningtyas; Agus Sabdono
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

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The present study aimed to propose a technical strategy of sampling for obtaining microbiological samples from liquid biomedical wastes generated by hospitals in Semarang City, Central Java Province, Indonesia.  The samples will be used to evaluate bioremediation ability of the hydrolytic bacteria isolated from the waste. It was hoped that the proposed strategy could help to guide researchers about appropriate sampling practices on hospital biomedical wastes from IPAL of Indonesian hospitals. As mandated by the Indonesian law, the handling of hospital liquid waste in Indonesia should follow Permenkes No.1204/2004, while the liquid waste standard should meet the Kepmen LH No.58/1995, reaffirmed by Perda Provinsi Jawa Tengah No.10/2004 about liquid waste standard issued by the Governor of Central Java. Based on formal regulations and safety aspects, it is proposed that the strategy for a proper microbiological sampling practice in terms of hospital liquid biomedical waste should include: (1) Permit letter from hospital where sampling location is; (2) Relevant sampling method based on research purpose (3) Standard, anti-leaked, properly labelled sampling containers(4) Proper sampling equipment based on sampling purpose (5) Adequate sampling transport equipment; (6) The use of standard personal protection equipment (PPE); and (7) Vaccine for the sampling workers (hepatitis B vaccination ismandatory).  Keywords: microbiological sampling, hospital biomedical waste, microbial bioremediation, sampling strategy,Indonesian sampling regulation
Co-Authors Agus Sabdono Andri Sukeksi Anisa Dian Cahyani Budi Santosa Cinta Ayu Bunga Shafira Fitri Nurul Aeni Muhammad Evy Prastiyanto Nur Azizah Sakti Imam Muchlissin Sri Darmawati Sri Sinto Dewi Stalis Norma Ethica Yuwanda Bagaskara