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All Journal PROSIDING SEMINAR NASIONAL Science and Technology Indonesia Jurnal Biomedika Prosiding Seminar Nasional Unimus
Muhammad Evy Prastiyanto
Unknown Affiliation
Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health

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Kecepatan Pertumbuhan Mycobacterium tuberculosis pada media Ogawa dengan Bahan Dasar Telur yang Berbeda Muhammad Evy Prastiyanto; Sri Darmawati; Iin Inayatul Karomah
Biomedika Vol 10 No 1 (2017): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (651.4 KB) | DOI: 10.31001/biomedika.v10i1.230

Abstract

Tuberkulosis adalah penyakit infeksi menular, kronik dan dapat menyebabkan kematian yang disebabkan oleh Mycobacterium tuberculosis. Diagnosa tuberkulosis dilakukan dengan berbagai cara, salah satunya dengan kultur pada media Ogawa. Penelitian ini bertujuan untuk mengetahui kecepatan pertumbuhan M. tuberculosis pada media ogawa dengan bahan dasar telur puyuh, telur bebek, telur enthok, dan telur ayam kampung. Metode yang dilakukan yaitu sputum BTA 3+ yang didapatkan dari Balai Kesehatan Paru Masyarakat (BKPM) Semarang diolah dengan metode kubica, kemudian diinokulasi pada media ogawa dengan bahan dasar telur yang berbeda. Pengamatan kecepatan pertumbuham M. tuberculosis dilakukan setiap hari. Hasil penelitian menunjukan ada perbedaan yang signifikan pada ke empat telur terhadap pertumbuhan M. tuberculosis pada media ogawa. Hasil penelitian menunjukkan bahwa media ogawa dengan bahan dasar telur puyuh dan entok dapat menumbuhkan M. tuberculosis lebih cepat dibanding dengan bahan dasar telur ayam kampung dan telur bebek. Media ogawa berbahan dasar telur puyuh dan telur entok menunjukkan rata-rata waktu pertumbuhan tercepat yaitu 17 hari. Sedangkan bahan dasar telur ayam kampung 20-21 hari dan bahan dasar telur bebek 23-24 hari.
Molecular Characterization And Hemagglutination Activities of Flagellin Protein of Salmonella typhi Sri Darmawati; Budi Santosa; Muhammad Evy Prastiyanto; Ragil Saptaningtyas
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2016: Proceeding of International Seminar on Education Technology (ISET) 2016
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Abstract. The purposes of this research are for molecular characterization and hemagglutination activity test of flagellinprotein of Salmonella typhi. The research samples consist of 7 strains of S. typhi isolates from Central Java (5 strains from Semarang city, 1 strain from Salatiga and 1 strain from Magelang) and 2 strains of S. typhi from Yogyakarta (Doctor Sardjito Hospital and Bethesda Hospital). The undertaking procedures are: 1) PCR and sequencing of fliC genes using primer LPW 1856 and LPW 1857.2) Isolation and separation of flagellin protein using SDS-PAGE. 3) Hemagglutination Activity Test upon human erythrocytes of blood group A, B, AB and O.The results show that 8 strains of S. typhi have a fliC gene size of 1452 to 1488 bp including serovar H1-d, and 1 strain with the size of 1267 bp including serovar H1-J. Flagella protein resulted from SDS-PAGE protein consists of 1-2 major proteins and 1-3 minor proteins with a molecular weight of 16-116 kDa. The results of hemagglutination activity test of flagellin protein show that there are 3 strains of S. typhi (MG-1, SA02.2 and BET) which are able to agglutinate human erythrocytes of bloodgroup A, B, AB and O (2-64HA), 6 other strains show various hemagglutination activities varied
AKTIVITAS ANTIBAKTERI MADU TERHADAP BAKTERI MULTI DRUG RESISTANT Salmonella typhi DAN METHICILLIN-RESISTANT Staphylococcus aureus Rahma Asriani Panjaitan; Sri Darmawati; Muhammad Evy Prastiyanto
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

This study aimed to analyze the antibacterial activity of Palm oil Honey against Multi  Drug  Resistant  Salmonella  typhi  and  Methicillin-Resistant Staphylococcus aureus by concentration 50%, 60%, 70%, 80%, 90% and 100%. This  research  was  an  experimental  test  with  Posttest  Only  Control  Group Design in an in vitro manner using well diffusion method techniques. The well diffusion method used MHA which made by the well of 5 mm diameter and inserted 200 µ L sample then incubated 35 ± 20C for 24 hours. The results of the study showed the antibacterial activity of Palm oil Honey with zone inhibition against MDR S.typhi 11.4 mm (concentration 90%), 13.4 mm (concentration 100%) and against MRSA 11.7 mm (concentration 100%). The results of zone inhibition of Palm oil honey with concentration 100% against MDR S.typhi was larger than the  zone inhibition  against MRSA. The palm oil honey inhibition zone  against  S.typhi  MDR  bacteria  compared  with  sulfamethoxazole  (SXT) antibiotics with 25 mm inhibition zones was included in the intermediate category and the palm oil honey inhibition zone against MRSA bacteria compared with Tetracycline (TE) antibiotics with 23 mm inhibition zone was included in resistant category. Keywords: Antibacterial Activity, MDR S. typhi, MRSA, Honey, Inhibition Zone
DETEKSI GEN Coa PADA Staphylococcus aureus YANG DIISOLASI DARI SUSU SAPI MURNI Rizka Ayu Wahyuni; Sri Darmawati; Muhammad Evy Prastiyanto
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Tujuan penelitian ini untuk mendeteksi gen Coa pada S. aureus yang diisolasi dari susu sapi murni di Kelurahan Gedawang. Jenis penelitian ini adalah penelitian deskriptif. Bakteri S. aureus di isolasi dari 15 sampel susu sapi murni. Deteksi gen Coa S. aureus menggunakan primer spesifik  Forward (5´-ATAGAGATGCTGGTACAGG-´3) dan primer Coa Reverse (5´GCTTCCGATTGTTCGATGC-3´).Jumlah isolat S. aureus yang didapatkan dari Isolasi Bakteri sebanyak 3 sampel positif yang memiliki gen Coa dengan hasil produk 756 bp. Hasil pada penelitian ini menunjukkan hasil positif gen Coa sebesar 100%.   Keywords: Staphylococcus aureus, Susu, gen Coa
Uji Aktivitas Antibakteri Ekstrak Etanol Kulit Daging Buah Siwalan (Borassus flabellifer) Terhadap Pertumbuhan Streptococcus mutans Mella Aprilia; Ayu Rahmawati Sulistyaningtyas; Muhammad Evy Prastiyanto
Prosiding Seminar Nasional Unimus Vol 4 (2021): Inovasi Riset dan Pengabdian Masyarakat Post Pandemi Covid-19 Menuju Indonesia Tangguh
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Bakteri Streptococcus mutans adalah bakteri patogen penyebab karies gigi. Penggunaan antibiotik yangtidak tepat dalam pengobatan infeksi dapat meyebabkan resistensi, sehingga dibutuhkan antibiotikdari bahan alam yng mempunyai daya kerja optimal dan relatif aman. Kulit daging buah siwalanmengandung bahan aktif saponin, tanin, flavonoid dan terpenoid  yang berguna sebagai antibakteri.Penelitian ini dilakukan untuk mengetahui daya hambat ekstrak etanol kulit daging buah siwalan .Jenis penelitian yang dilakukan adalah penelitian eksperimental, penelitian ini menggunakankonsentrasi ekstrak kulit daging buah siwalan  200mg/ml, 400mg/ml, 600mg/ml, 800mg/ml, 1000mg/mlyang diuji dengan bakteri Streptococcus mutans. Metode ekstraksi yang digunakan dalam penelitianini yaitu maserasi dengan pelarut etanol 96% dan diuji daya hambat menggunakan metode sumuran,uji Minimum Inhibition Concentration (MIC) serta Minimum Bacterial Concentration MBCmenggunakan media BAP. Hasil penelitian menunjukkan bahwa ekstrak kulit daging buah siwalandapat menghambat pertumbuhan bakteri Streptococcus mutans dengan rata-rata diameter zonahambat 16,86 mm, 17,72 mm, 18,56 mm, 19,4 mm, 20,16 mm, semakin tinggi konsentrasi ekstrak kulitdaging buah siwalan maka semakin tinggi daya hambat terhadap pertumbuhan bakteri Streptococcusmutans Kata Kunci : kulit daging buah,MBC,MIC, Siwalan,Streptococcus mutans, zona hambat
Phytochemical Profile, Antioxidant Activity and Anticancer Activity of Gamma-Irradiated Black Rice Bran (Oryza sativa L.) Ethanolic Extract: In-Vitro and In-Silico Study Rukmana, Rizal Maarif; Silfarohana, Rantika; Putra, Aditya Dwi Permana; Devi Safrina; Dian Susanti; Nur Rahmawati Wijaya; M Bakti Samsu Adi; Muhammad Evy Prastiyanto; Yanuar Ashari Cahyaningrum; Rina Nurmaulawati; Weka Sidha Bhagawan
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.628-643

Abstract

Black rice is a food crop with black pigments, which helps reduce the risk of various diseases and improve health. One way to improve food quality and avoid food contaminants is by irradiating. This study evaluated the phytochemical profile, antioxidant activity, and anticancer activity of irradiated black rice bran extract (IBRBE) in vitro and in silico. Black rice was irradiated with a Gamma cell 220 type irradiator at a 7.5 kGy/hour dose rate. Extraction of irradiated black rice bran was carried out using the maceration method. Phenolic and flavonoid components were quantified using the Folin–Ciocalteau and AlCl3 methods, respectively. Phytochemical compounds were identified by liquid chromatography-high-resolution mass spectrometry (LC HRMS). The antioxidant activity of IBRBE was carried out against 2,2-diphenyl-1picrylhydazyl (DPPH). The cytotoxic activity of IBRBE against WiDr cells (colorectal cancer) and Vero (nonhuman cell lines) used the MT test method. Prediction of the inhibitory mechanism of compounds in the extract against target proteins EGFR and GPX7 was carried out in silico. Total phenolics and flavonoids were 2.57 ± 0.28 mg GAE/g and 19.12 ± 0.18 μg QE/ml, respectively. Twenty-four types of active compounds were obtained in IBRBE. The results of antioxidant activity obtained an IC50 value of 1198.45 ± 92.86 μg/ml. IC50 in WiDr cells and Vero cells were obtained at 36.08 ± 11.71 μg/ml and 570.58 ± 130.25 μg/ml, respectively. In silico results, the compound 4 Dodecylbenzene sulfonic acid has the highest binding affinity to the EGFR protein, with a value of -5.9. Meanwhile, the Monoolein compound has the highest binding affinity to the GPX7 protein, with a value of -5.4.
Co-Authors Ayu Rahmawati Sulistyaningtyas Budi Santosa Devi Safrina Dian Susanti Iin Inayatul Karomah M Bakti Samsu Adi Mella Aprilia Nur Rahmawati Wijaya Putra, Aditya Dwi Permana Ragil Saptaningtyas Rahma Asriani Panjaitan Rina Nurmaulawati Rizal Maarif Rukmana, Rizal Maarif Rizka Ayu Wahyuni Silfarohana, Rantika Sri Darmawati Weka Sidha Bhagawan Yanuar Ashari Cahyaningrum