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All Journal Jurnal Rekayasa Lingkungan Makara Journal of Science
Ghozali, Ali Aulia
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Civil Engineering, Building, Construction & Architecture Engineering Environmental Science

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Highly Sensitive Phenol Biosensor Utilizing Selected Bacillus Biofilm Through an Electrochemical Method Ariyanti, Dita; Iswantini, Dyah; Sugita, Purwantiningsih; Nurhidayat, Novik; Effendi, Hefni; Ghozali, Ali Aulia; Kurniawan, Yehezkiel Steven
Makara Journal of Science Vol. 24, No. 1
Publisher : UI Scholars Hub

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An eco-friendly phenol biosensor from Bacillus biofilm was prepared and investigated. The biofilm, which produced tyrosinase enzyme, was successfully immobilized on a screen-printed carbon electrode surface. A total of 72 Bacillus isolates were utilized because of their capability to produce tyrosinase enzyme in tyrosine media. Among them, Bacillus isolate code 100 was selected because it produced an adequate amount of tyrosinase enzyme and a high potentiostat current. The response surface methodology was also used to optimize the phenol sensing condition through an electrochemical method. Results showed that the optimum condition was achieved after 6 days on a phosphate buffer solution (pH of 8), with an optical density of 0.33. Furthermore, the limits of detection and quantification were 3.0 and 13 ng/L, respectively. The measurements of precision yielded a relative standard deviation of < 5%, which is remarkable. Although the biosensor material was used for 35 days, the current throughout was still maintained at 90%, indicating that the evaluated biosensor material has the potential to be used for phenol monitoring on environmental samples in the near future.
EFEKTIVITAS DAN MODEL ISOTERM ADSORPSI Fe DAN Mn OLEH MATA LELE (Lemna minor) Ghozali, Ali Aulia; Sohra, Lulu Alfatih; Eviane, Dewi
Jurnal Rekayasa Lingkungan Vol. 23 No. 2 (2023)
Publisher : Institut Teknologi Yogyakarta

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Salah satu teknik untuk menurunkan kadar logam dalam air adalah fitoremediasi, dan mata lele (Lemna minor) berpotensi untuk digunakan sebagai agen bioremediator. Penelitian ini bertujuan mengukur efektivitas tanaman mata lele dalam meremediasi kandungan logam dalam air sumur, serta mengevaluasi model isoterm adsorpsinya. Penelitian ini dilaksanakan dari bulan November 2022 hingga Maret 2023. Sampel diambil secara komposit dari sumur warga di Dukuh Baran, Kalurahan Srihardono. Variabel yang diukur adalah pH, kadar Fe dan Mn, kesadahan, temperatur, serta coli tinja. Hasil penelitian menunjukkan pada kondisi eksisting air sumur sampling, tanaman mata lele mampu secara efektif menurunkan konsentrasi Mn pada air sumur, sebaliknya tidak ditemukan perbedaan yang signifikan pada konsentrasi Fe, sebab konsentrasinya yang memang sudah rendah. Pada konsentrasi 100 ppm untuk kedua logam, mata lele mampu mengadsorpsi kedua logam dengan efektivitas penjerapan 86-94%. Kajian model isoterm adsorpsi menunjukkan logam Mn mampu termodelkan dengan baik dengan model isoterm adsorpsi Freundlich, sebaliknya logam Fe tidak termodelkan dengan baik.
Co-Authors Dita Ariyanti Dyah Iswantini Eviane, Dewi Hefni Effendi Kurniawan, Yehezkiel Steven Novik Nurhidayat Purwantiningsih Sugita Sohra, Lulu Alfatih