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PENDUGAAN UMUR SIMPAN DAN PERUBAHAN ASAM LEMAK ABON JAMUR TIRAM (Pleurotus ostreatus) SELAMA PENYIMPANAN Sudarminto Setyo Yuwono; Nundiah Zuhrohfi Immaroh; Harijono
Jurnal Teknologi Pertanian Vol. 24 No. 3 (2023)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtp.2023.024.03.7

Abstract

Jamur tiram dapat dimanfaatkan dalam pengembangan produk abon. Abon, salah satu produk pangan kering, umumnya berbahan baku daging melalui proses perebusan dan penggorengan. Selama penyimpanan, abon jamur tiram mengalami penurunan mutu, seperti munculnya aroma tengik akibat reaksi oksidasi. Penentuan umur simpan atau masa kadaluarsa sangat dibutuhkan agar diketahui batasan waktu produk dalam kondisi layak konsumsi. Tujuan dari penelitian ini adalah untuk mengetahui umur simpan abon jamur tiram serta perubahan asam lemak sebelum dan sesudah penyimpanan. Penentuan umur simpan abon jamur tiram menggunakan metode Accelerated Shelf Life Testing (ASLT) dengan persamaan Arrhenius, penyimpanan pada suhu 35, 45, dan 55°C. Hasil penelitian menunjukkan 60% panelis terlatih menolak abon jamur tiram pada hari ke-147. Mutu kritis warna L* 33,2; a* 18,8; b* 16, nilai peroksida 16,45Meq/kg,  asam lemak bebas 0,83 dan TBA 3,46 mg/kg. Penentuan umur simpan menggunakan nilai energi aktivasi terendah, yaitu parameter TBA sebesar 3982,931kal/mol dengan persamaan regresi linier dari plot Arrhenius y = −2.005,5x + 1,5173 dan nilai R2 0,908. Umur simpan abon jamur tiram apabila disimpan pada suhu 25, 27, dan 30°C berturut-turut adalah 515,34; 489,49; dan 458,14 hari. Terdapat enam senyawa baru yang muncul selama penyimpanan, yaitu Octanoic acid, methyl ester (Cas) Methyl octanoate, Pentadecanoic acid, 14-methyl-, methyl ester (Cas) Methyl 14-Methyl-Pentadecanoate, Nonanoic Acid (Cas) Nonoic Acid, 2-Isononenal (Cas) Branched Chain 2-Nonenal, Heptanoic Acid, 3,6,6-Trimethyl-, Methyl Ester (Cas) 3,6,6-Trimethylheptanoic Acid Methyl Ester, 9-Octadecenoic Acid (Z)- (Cas) Oleic Acid, 13-Docosenoic Acid, Methyl Ester (Cas) Methyl 13-Docosenoate. Jenis asam lemak tersebut dapat menimbulkan aroma tengik.
Study of Antihypertensive Activity from Red Quinoa Seed Protein Hydrolysate Digested by Various Protease Enzymes Suleman, Dininurilmi Putri; Harijono; Hsu, Jue-Liang
HAYATI Journal of Biosciences Vol. 31 No. 1 (2024): January 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.1.192-199

Abstract

Proteolytic enzymes are widely used to produce protein hydrolysates that contain bioactive peptides. Some of bioactive peptides are known inhibit the angiotensin-I-converting enzyme (ACE, EC 3.4.15.1) and act as human antihypertensive. Therefore, this study aims to produce protein hydrolysates via 16 hours of digestion process using Chenopodium formosanum (red quinoa) seed and the proteases, namely pepsin, trypsin, α-chymotrypsin, and thermolysin. The hydrolysates profiles and ACE-I inhibitory activity were analyzed using reversed phase-high performance liquid chromatography (RP-HPLC). The SDS-PAGE was also used to analyze the main storage protein in red quinoa seed, identified as being 11S seed storage globulin. Meanwhile, the ACE inhibitor activities of red quinoa seed protein (RQSP) produced by various proteases include the hydrolysate of pepsin 17.03% ± 3.88%, trypsin 42.67% ± 3.19%, α-chymotrypsin 72.71% ± 2.85% and thermolysin 77.67% ± 0.98%. These results show that red quinoa seed protein is a potential source of significant ACE inhibitor activity when hydrolyzed with α-chymotrypsin and thermolysin.