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All Journal Muhammadiyah Journal of Nutrition and Food Science (MJNF) Pharmaceutical And Biomedical Sciences Journal (PBSJ)
Suri, Auliyani Andam
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Profil Mikrobiota ASI dan Perannya terhadap Saluran Cerna Bayi Syahniar, Rike; Suri, Auliyani Andam
Muhammadiyah Journal of Nutrition and Food Science (MJNF) Vol 1, No 1 (2020): Muhammadiyah Journal of Nutrition and Food Science (MJNF)
Publisher : Faculty of Medicine and Health Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.762 KB) | DOI: 10.24853/mjnf.1.1.8-17

Abstract

Latar Belakang: Air susu ibu (ASI) mengandung mikrobiota dalam jumlah banyak yang mewakili bakteri komensal, mutualistik, dan bakteri yang berpotensi sebagai probiotik pada saluran cerna bayi. Hasil: Filum bakteri yang paling dominan dalam ASI antara lain Proteobacteria dan Firmicutes sedangkan pada tingkat genus yaitu Staphylococcus, Pseudomonas, Streptococcus dan Lactobacillus. Diantara berbagai mikrobiota yang terdapat dalam ASI, Lactobacillus dan Bifidobacterium berpotensi sebagai probiotik. Kesimpulan: Mikrobiota usus memberikan fungsi metabolisme, dan proteksi yang bermanfaat seperti peningkatan kapasitas pencernaan, produksi asam lemak rantai pendek dan vitamin, pengaturan struktur mukosa dan sistem kekebalan tubuh serta resistensi kolonisasi terhadap patogen.
Studi awal: Identifikasi Keragaman Gen Bakteri Saliva pada Wanita Usia Lanjut Menggunakan Teknologi 16S Ribosome Sequencing Oxford Nanopore Adhiyanto, Chris; Hendarmin, Laifa A; Harriyati, Zeti; Suwarsono, Erike A. Suwarsono A; Suri, Auliyani Andam; Nugraha, Yudhi; Gunarti, Dwirini Retno
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol 7, No 1 (2025)
Publisher : UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/pbsj.v7i1.46752

Abstract

The elderly are a population particularly susceptible to diseases resulting from the decline in immune system function. The mouth is one of the areas of the body that is the entry point for microorganisms into the body. It is known that the oral microbiome greatly affects oral and systemic health. The oral bacterial profile is important to understand its role in maintaining health and causing disease. Therefore, maintaining oral health is very important, especially in our study of the elderly. Utilizing advanced sequencing techniques such as Oxford Nanopore Technology (ONT) offers the opportunity to reveal bacterial diversity comprehensively. This study aims to identify the oral microbiome in a group of elderly women. Knowledge of the oral microbiota profile could help spread awareness of the benefits of maintaining good dental hygiene, especially in older adults. Methods: Seven elderly housewives had their unstimulated saliva samples taken in the morning and kept at -80°C. The phenol-chloroform technique was used to isolate the DNA, and a Qubit Fluorometer was used to measure purity and concentration. By employing ONT for 16S rRNA sequencing and Epi2Me software for analysis, taxonomic mapping of the bacterial diversity was accomplished.  Results: According to the taxonomic analysis, Streptococcus and Veillonella were the most common genera in the samples. It was common to find species like Veillonella parvula, Veillonella atypica, Streptococcus mitis, and Streptococcus rubneri. These genera contributed to the dynamics of oral biofilms by exhibiting complex ecological interactions. With advanced sequencing technologies, this work highlights the diversity of oral bacteria in elderly women in Indonesia while providing a new perspective. Notwithstanding a few limitations, these findings provide insight into the ecological dynamics of the oral microbiota, potentially leading to clinical applications in the treatment and prevention of disease. 
Co-Authors CHRIS ADHIYANTO Dwirini Retno Gunarti Laifa Annisa Hendarmin Nugraha, Yudhi Rike Syahniar Suwarsono, Erike A. Suwarsono A Zeti Harriyati