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All Journal INDONESIAN JOURNAL OF PHARMACY Jurnal Farmasi Sains dan Komunitas Indonesian Journal of Chemistry Abdimas Altruis: Jurnal Pengabdian Kepada Masyarakat
Christofori Maria Ratna Rini Nastiti
Faculty Of Pharmacy, Sanata Dharma University, Yogyakarta
Humanities Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Medicine & Pharmacology Social Sciences Other

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Assay method validation of triamcinolone acetonide (TA) to support the investigation of TA-loaded nanoparticles Nastiti, Christofori Maria Ratna Rini
Indonesian Journal of Pharmacy Vol 20 No 4, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.127 KB) | DOI: 10.14499/indonesianjpharm0iss0pp178-184

Abstract

The aim of this study was to develop the valid analytical method which used for the assay of triamcinolone acetonide (TA) in the investigation of TA-loaded nanoparticle formulations. High Performance Liquid Chromatography (HPLC) method was applied in this study by using an Econosil column, C18 10 'm, 250 x 4.6 mm (Alltech Associates Inc, PA, USA) as the stationary phase. The mobile phase consisted of a composition of acetonitrile (ACN) and 20mM phosphate buffer solution (pH 4.2) in the proportion of 50:50 v/v. The HPLC assay of TA was validated for selectivity, linearity, precision, recovery (accuracy), sensitivity and stability of TA during the assay. Results showed that the concentration of TA in the samples can be determined against the standard in the concentration range of calibration curve. The system precision and level of recovery were considered to be acceptable, and the method was selective and sensitive.Key words: triamcinolone acetonide, assay, validation
Analytical Method Validation and Formula Optimization of Topical Nanoemulsion Formulation Containing Resveratrol Christofori Maria Ratna Rini Nastiti; Florentinus Dika Octa Riswanto
Indonesian Journal of Chemistry Vol 21, No 5 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.63730

Abstract

Resveratrol (RSV), a natural lipophilic phytoalexin, was reported as an antioxidant and anti-inflammatory agent, which has the potential to cure diabetic wounds. However, several studies suggested the limitation of RSV, such as poor aqueous solubility, poor stability, and poor oral bioavailability. To overcome the issues, RSV was formulated as a topical nanoemulsion. It is important to ensure the quality of the dosage form by evaluating RSV load in the nanoformulation and optimizing the formula. A reversed-phase HPLC method was developed and validated prior to the load determination of RSV in the nanoemulsion formulation. The composition of triacetin-eugenol, Kolliphor® RH 40, and Transcutol® was further optimized by employing a Box-Behnken Design (BBD) to achieve the optimum composition with expected viscosity and RSV load. The HPLC method for determining RSV load was successfully validated for parameters of selectivity with the resolution of 8.487, linearity and range (r = 0.9979), precision (0.12% of RSD), accuracy (109–110% of recovery), the limit of detection (0.574 µg/mL), and limit of quantitation (1.740 µg/mL). The result of formula optimization was promising, showing the optimum composition of triacetin-eugenol, Kolliphor® RH 40, and Transcutol® at 4.44 g, 30.97 g, and 11.39 g, respectively.
PEMERIKSAAN PROFIL LIPID PADA JEMAAT LANSIA SALAH SATU GEREJA KRISTEN DI YOGYAKARTA Patramurti, Christine; Triwulandari, Erna; Nastiti, Christofori Maria Ratna Rini
ABDIMAS ALTRUIS: Jurnal Pengabdian Kepada Masyarakat Vol 6, No 2 (2023): Oktober 2023
Publisher : Universitas Sanata Dharma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/aa.v6i2.6829

Abstract

Lipid profile examination including total cholesterol level, triglycerides level and high-density lipoprotein cholesterol reduction is essential to be done as a routine activity quinquennially to prevent someone from the risk of cardiovascular diseases, diabetes and obesity. This kind of examination is mandatory for the elderly people to maintain their quality of life. In order to support the government program on the prevention of the cardiovascular diseases, a team of social service of Faculty of Pharmacy Universitas Sanata Dharma (FFUSD) was organizing an event of lipid profile examination in one of Christian church located in Kalasan, Yogyakarta. As many as 35 elderly people were enthusiastically participating on the event. A nurse from Poliklinik Sanata Dharma was appointed to take the blood samples, which were further taken to the Biochemistry Laboratory in FFUSD to get analysed. The results were sent to the participants in private. The overall blood lipid profiles of most participants showed good level of quality, indicating that they have been trying to manage their life healthily. However, as much as 62. 8% of participants showed a low level of the high-density lipoprotein cholesterol.  This was believed due to the food intake which was still rich in oil and fat.  The event was very succesful and the responses were very positive. The participants felt assisted with a fast and accurate results sent.
Evaluation of Diabetic Wound Healing Activity of Novel Quercetin Topical Preparations Nastiti, Christofori Maria Ratna Rini; Michelina, Ellisia; Wijayanti, Fabiola Rani; Gani, Michael Raharja
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 21, No 1 (2024)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/jpsc.007288

Abstract

Diabetic wound needs effective pharmacotherapy in order to accelerate the wound healing process and to prevent further infection. Quercetin (QUE) is one of flavonols which ispotential as an active ingredient for diabetic wound therapy, due to its anti-inflammatory and antioxidant activities.  This current study evidenced wound healing activity of QUE topical preparations on the open wound of diabetic-induced subjects preclinically. The investigation focused on topical administration of QUE nanoemulgels and QUE micellar gels (0.2% w/w of QUE) on groups of diabetic-induced male Wistar rats. The quality of preparations in terms of organoleptic performance, pH, viscosity and spread-ability was physically characterized prior to topical administration to the wound. Punch biopsy (5mm) was applied to create the open wound. The visual observation of wound contraction area (in mm2) was carried out every three days. On the day 12th, after sacrificing the subjects, the wounded tissues were removed, fixed in 10% formalin and prepared for histology examination-hematoxylin and eosin staining. The results showed that the QUE nanoemulgels and QUE micellar gels were relatively comparable onthe physical quality as well as the wound healing activity. Their wound healing activities were remarkably faster compared to the untreated control group and it was showed fromthe histology study that the QUE nanoemulgels and QUE micellar gels successfully accelerate the wound healing process to the proliferation step if it was compared the untreated. In conclusion, QUE nanoemulgels and QUE micellar gels were potential to develop to accelerate the diabetic wound healing process.    
Co-Authors Christine Patramurti Florentinus Dika Octa Riswanto Gani, Michael Raharja Michelina, Ellisia Triwulandari, Erna Wijayanti, Fabiola Rani