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All Journal Microbiology Indonesia Journal of Tropical Biodiversity and Biotechnology Journal of Agri-Food Science and Technology (JAFoST) Jurnal Atma Inovasia SERVIRISMA: Jurnal Pengabdian kepada Masyarakat
HARTAJANIE, LAKSMI
Jurusan Teknologi Pangan, Fakultas Teknologi Pertanian, Universitas Katolik Soegijapranata
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Other

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Journal : Microbiology Indonesia

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THE EFFECT OF CARBON AND NITROGEN SUPPLEMENTATION ON BACTERIOCIN PRODUCTION OF LACTIC ACID BACTERIA FROM PICKLED YELLOW BAMBOO SHOOTS (Dendrocalamus asper) LAKSMI HARTAJANIE; LINDAYANI LINDAYANI; LORENTIA SANTOSO
Microbiology Indonesia Vol. 12 No. 1 (2018): March 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3837.952 KB) | DOI: 10.5454/mi.12.1.2

Abstract

Six selected lactic acid bacteria (LAB) isolates from pickled Yellow Betung bamboo shoots were grown in de Mann Rogosa Sharpe-Broth (MRSB) media with different supplementation combination. The cell-free supernatant were evaluated for their ability to produce bacteriocin  by adjusting its pH to 6.0 in order to remove organic acid effects. The bacteriocin activity was  assayed by agar-well diffusion method. The inhibitory activity calculated in Activity Unit (AU in mm2 mL-1) of bacteriocins. The aims of this paper is to explore the effect of different medium compositions on bacteriocin production and its inhibitory activity against pathogenic bacteria (Listeria monocytogenes FNCC 0156, Staphylococcus aureus FNCC 0047, and Escherichia coli FNCC 0091).Supplementations of carbon and nitrogen sources induced production of bacteriocins. LAB isolates grown in media without supplementation could not produce bacteriocins.  Growth of isolate D44 in the presence of 2% of glucose and 2% of yeast extract yielded the largest bacteriocin inhibitory activity levels of 3179 AU mL-1 against Listeria monocytogenes FNCC 0156, 4663 AU mL-1 against Staphylococcus aureus FNCC 0047, and 3109 AU mL-1 against Escherichia coli FNCC 0091.
Probiotic Potential of Lactic Acid Bacteria from Yellow Bamboo Shoot Fermentation using 2.5% and 5% Brine at Room Temperature LINDAYANI LINDAYANI; LAKSMI HARTAJANIE; MONIKA PALUPI MURNIATI
Microbiology Indonesia Vol. 12 No. 1 (2018): March 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1089.648 KB) | DOI: 10.5454/mi.12.1.5

Abstract

Yellow bamboo shoot is a popular food material especially in Semarang because it is used as filling for lumpia (authentic food of Semarang). Beside used as filling, yellow bamboo shoot commonly known by Indonesian people. Considering bamboo shoot often processed into fermented traditional food, yellow bamboo shoot also potential to be examined as source of fermented food producing lactic acid bacteria. Lactic acid bacteria still become the most beneficial microorganisms associated with gastrointestinal system and moreover for obesity diet. The aim of this research is to study the probiotic potential of lactic acid bacteria produced from yellow bamboo shoot fermentation in 2.5% and 5% brine under room temperature (30 °C). From isolation, 22 single colonies obtained from 2.5% brine and 27 isolates obtained from 5% brine. The morphology and physiology analysis resulted in Lactobacillus and Streptococcus genus. All isolates were tested subsequently for probiotic potential. Based on the result, more than 50% identified isolates have probiotic potential.
Co-Authors - Lindayani Anto Budiharjo Dyah Wulandari Dyah Wulandari Elizabeth Lucky Maretha Sitinjak Elizabeth Lucky Maretha Sitinjak Florentinus Budi Setiawan Florentinus Budi Setiawan Lindayani Lindayani Lindayani LINDAYANI LINDAYANI Lindayani, Lindayani LORENTIA SANTOSO Magfiroh, Yani Maretha, Elizabeth Lucky Maulana, Anand Reyna Mawarni, Risa Arum MONIKA PALUPI MURNIATI Nurhayati Nurhayati Shabrina, Jauhara Shandy Jannifer Matitaputty Shandy Jannifer Matitaputty Wijanarka Wijanarka