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Kristamtini, Kristamtini
Balai Pengkajian Teknologi Pertanian (BPTP) Yogyakarta
Agriculture, Biological Sciences & Forestry

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Keragaman Genetik dan Heritabilitas Beberapa Karakter Agronomi Populasi Galur F4 Padi Beras Hitam Widyayanti, Setyorini; Basunanda, Panjisakti; Mitrowihardjo, Suyadi; Kristamtini, Kristamtini
Jurnal Penelitian Pertanian Tanaman Pangan Vol 1, No 3 (2017): Desember 2017
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (405.806 KB) | DOI: 10.21082/jpptp.v1n3.2017.p191-199

Abstract

The F4 black rice progenies and their two parents’ varieties were evaluated for their heritability and genetic variability at Padasan, Pakembinangun, Pakem, Sleman, Yogyakarta from May until September 2016. The purpose of this study is to determine genetic diversity, heritability estimates and genetic advance.The genetic materials used in this study consisted of 48 lines of F4 with their parents, local black rice and Situbagendit. The experiment was arranged in a randomized block designed with two replications. Seeds of each F4 progenies and their parents were sown 1 m × 2 m length of plot with 20 cm × 20 cm plant spacing. Genetic parameters estimatesd were calculated for heritability and genetic advance. Plant population of each F4 progenies and their parents were 40 plants in each replication.  The result showed that there were 11 progenies had an early day to maturity potential, 21 F4 progenies had a moderate plant height (60-95 cm), 13 progenies had a potential number of tillers per hill (10.26 to 18.76), 9 F4 progenies had a high number of filled grains per panicle (> 80% filled grains), and 7 F4 progenies had a potential of 1000-grain weight (g). Plant height had the highest coefficient of variability. Days to flowering, days to maturity, and pericarb color had the highest heritability estimates followed by plant height (70.6%). Plant height and pericarb color had the highest genetic advances, namely 21.7% and 49.2%, respectively. In relation to improve agronomic characters, plant height and pericarb color had a highest heritability and genetic advance also a wide genetic variability.
Korelasi Kandungan Antosianin Total dengan Parameter Warna (L*, a*, dan b*) dan Penanda Mikrosatelit pada Beras Hitam Lokal Kristamtini, Kristamtini; Taryono, Taryono; Basunanda, Panjisakti; Murti, Rudi Hari
Jurnal Penelitian Pertanian Tanaman Pangan Vol 1, No 2 (2017): Agustus 2017
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (469.063 KB) | DOI: 10.21082/jpptp.v1n2.2017.p115-124

Abstract

Black rice is a functional food for its  high anthocyanin contents expressed on purple-black rice color. The study aimed to determine the correlation between: total anthocyanin content with  L *, a *, and b * color variable, total anthocyanin content with color morphology and total anthocyanin content microsatellite markers. Eleven local black rice varieties  from Yogyakarta Assesment Institute of Agriculture Technology collections and two varieties of white rice (Situbagendit and Inpari 6 Jete) were planted on plastic pots in a green house. The tried was arranged   completely randomized design with five replications. At three weeks after planting, the leaf samples were are taken for DNA isolation and further PCR amplifications  using four microsatellite markers (RM 180, RM 220, RM 224 and RM 252). The black rice grain were observed based on color morphology using a Chromameter and analyzed  for then anthocyanin contents. Regression, correlation, and path analysis were conducted to determine the relationship between total anthocyanin content and L* a* b * color variable, color morphology, and microsatellite markers. The results showed that there is correlation between total anthocyanin content  with color parameters (L* = -0,568,  a* = -0,561, b*= 0,844).  Total anthocyanin content is correlation too with color scor of morfology and total   microsatellite markers ((RM 180 =-0,419, RM 220 =0,27, RM 224 =-0,493, RM 252 =0,265) and color scor of morphology  = -0,442. Selection of high-anthocyanin content of rice can use the color variable criterion (L*an  a* low, and  b * high). Microsatellite markers RM 220 and RM 252 can be used as moleculer markers for rice of high anthocyanin content.
Co-Authors Mitrowihardjo, Suyadi Murti, Rudi Hari Panjisakti Basunanda Taryono, Taryono Widyayanti, Setyorini