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Chronic Respiratory Disease (Crd) of Chicken ., Soeripto
Indonesian Bulletin of Animal and Veterinary Sciences Vol 19, No 3 (2009)
Publisher : Indonesian Animal Sciences Society

Chronic respiratory disease (CRD) of chicken is the most costly disease confronting poultry industries in the world. The economic losses due to CRD was estimated up to billions rupiahs per year in Indonesia, and in the USA was estimated up to hundred millions dollars per year. The losses mainly due to decreases of body weight gain, egg production, feed efficiencies, hatchabilities and Â increases Â ofÂ embryo Â mortality. Â The Â main Â causative agent Â of Â CRD Â is Mycoplasma Â gallisepticum (MG). Respiratory disturbances, excretion of nasal exudate, coughing, sneezing and hyperaemic of the conjunctiva are very often seen as the clinical signs. Pathological lesions are often found as inflammation of respiratory organs and more specific lesions are seen as inflammation and thickening of the airsac membranes with foci cheesy materials scattered around the airsacs. Diagnosis of CRD can be made by clinical symptoms, serology examination dan isolation of MG. Treatment, prevention and controls of CRD have been carried out for years, but cases of CRD are still present up to now. The MGTS11 vaccine as the third generation of CRD vaccine was reported to be effective for controlling CRD of chickens and potentially used as a tool for eradication programme of CRD in the future. Â Key words: Chronic respiratory disease, chicken

The use of sodium pyruvate and sodium hydroxide to increase the number of colonies and coloured antigen yield of Mycoplasma gallisepticum ., Soeripto
Indonesian Journal of Animal and Veterinary Sciences Vol 2, No 3 (1997)
Publisher : Indonesian Animal Sciences Society

A trial to increase the number of colonies and coloured antigen yield of Mycoplasma gallisepticum in mycoplasma broth medium was conducted by comparing the use of normal medium, medium with sodium pyruvate and medium with sodium hydroxide. The result showed that medium with Â§odium pyruvate had increased the number of colonies and antigen yield and was highly significant different (P< 0.01) compared to both normal medium and medium with sodium hydroxide. Medium with sodium hydroxide produced the number of colonies and antigen yield lower than medium with sodium pyruvate, but higher and was highly significant different (P< 0.01) compared Â to the normalmedium. Â Key words: Sodium pyruvate, sodium hydroxide, Mycoplasma gallisepticum antigen

Sensitivity of some local isolates of Mycoplasma gallisepticum against antibiotics Wahyuwardani, Sutiastuti; ., Soeripto
Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 1 (1998)
Publisher : Indonesian Animal Sciences Society

Sensitivity of five local isolates ofMycoplasma gallisepticum (MG) strain and two standard MG isolates obtained from Australia were tested against antibiotics of oxytetracycline, doxycycline, erythromycin, bacitracin, vancomycin, methicillin and penicillin using antibiogram disc method. The result showed that one, 2 and 3 local MG isolates were resistent to doxycycline, erythromycin and oxytetracycline respectively . MG isolate of ADA7 from Australia was found to be resistent to all antibiotics tested. None ofthe local MG isolates were sensitive against bacitracin, vancomycin, methicillin and penicillin. Â Key words: Mycoplasma gallisepticum, antibiogram disc

The efficacy of Tiamulin hydrogen fumarat 10% in the feed to prevent chronic respiratory disease in broiler chickens ., Soeripto
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 1 (2008)
Publisher : Indonesian Animal Sciences Society

Up to presence chronic respiratory disease (CRD) of chickens is still causing economic losses against poultry industries in the world. The purpose of this trial is to determine the efficacy and safety of a compatible dose of Tiamulin hydrogen fumarat 10% in combination with monensin for the control of CRD in broilers. A number of 630 day-old broilers were divided into 3 groups and each group was divided again into 7 subgroups of 30 equally sexed birds. Each subgroup was placed randomly in 2 chicken houses. Up to 3 weeks of age, chickens in Group I were fed with starter feed (SP1) containing 100 ppm monensin only without other treatment and used as control. Chickens in Group II were fed with SP1 feed containing 30 ppm Tiamulin hydrogen fumarat (3 â 6 mg/ kg BW) and 110 ppm amoxicillin, this feed is called SP1+, whereas chickens in Group III were administered with SP1 feed and treated with enrofloxacin liquid formulation 10% with a dose 0.5ml/L in drinking water for the first 5 days of life. Started from 22nd day until the end of the experiment at 32 days of age, all chickens in Groups I, II and III were fed with SP2 finisher feed containing neither monensin nor Tiamulin hydrogen fumarat. The results of the experiment showed that no statistical difference in bodyweight and feed conversions among the groups at 32 days of age but feed conversion in Group II was statistically different compared to those in Groups I and III at week 2. No clinical signs of toxic interaction of monensin combined with Tiamulin were observed. Lesions of airsacculitis and ascites occurred only in dead chickens of Groups I and III but not in chickens of Group II. The incidence of pneumonia in Group I occurred in all dead birds which is statistically different to Group II that had one lesion of pneumonia. Mycoplasma gallisepticum and Escherichia coli organisms were able to be isolated from the chickens that had pneumonia and ascites in Groups I and III only. The results of the experiment showed that combination of 30ppm Tiamulin hydrogen fumarat + 110 ppm amoxicillin is effective for preventing CRD in broilers and save if it is combined with 100 ppm monensin. Key Words: Tiamulin Hydrogen Fumarat, Chronic Respiratory Disease, Broilers

The efficacy of Tiamulin hydrogen fumarat 10% in the feed to prevent chronic respiratory disease in broiler chickens Soeripto .
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 1 (2008): MARCH 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Up to presence chronic respiratory disease (CRD) of chickens is still causing economic losses against poultry industries in the world. The purpose of this trial is to determine the efficacy and safety of a compatible dose of Tiamulin hydrogen fumarat 10% in combination with monensin for the control of CRD in broilers. A number of 630 day-old broilers were divided into 3 groups and each group was divided again into 7 subgroups of 30 equally sexed birds. Each subgroup was placed randomly in 2 chicken houses. Up to 3 weeks of age, chickens in Group I were fed with starter feed (SP1) containing 100 ppm monensin only without other treatment and used as control. Chickens in Group II were fed with SP1 feed containing 30 ppm Tiamulin hydrogen fumarat (3 – 6 mg/ kg BW) and 110 ppm amoxicillin, this feed is called SP1+, whereas chickens in Group III were administered with SP1 feed and treated with enrofloxacin liquid formulation 10% with a dose 0.5ml/L in drinking water for the first 5 days of life. Started from 22nd day until the end of the experiment at 32 days of age, all chickens in Groups I, II and III were fed with SP2 finisher feed containing neither monensin nor Tiamulin hydrogen fumarat. The results of the experiment showed that no statistical difference in bodyweight and feed conversions among the groups at 32 days of age but feed conversion in Group II was statistically different compared to those in Groups I and III at week 2. No clinical signs of toxic interaction of monensin combined with Tiamulin were observed. Lesions of airsacculitis and ascites occurred only in dead chickens of Groups I and III but not in chickens of Group II. The incidence of pneumonia in Group I occurred in all dead birds which is statistically different to Group II that had one lesion of pneumonia. Mycoplasma gallisepticum and Escherichia coli organisms were able to be isolated from the chickens that had pneumonia and ascites in Groups I and III only. The results of the experiment showed that combination of 30ppm Tiamulin hydrogen fumarat + 110 ppm amoxicillin is effective for preventing CRD in broilers and save if it is combined with 100 ppm monensin. Key Words: Tiamulin Hydrogen Fumarat, Chronic Respiratory Disease, Broilers

The use of sodium pyruvate and sodium hydroxide to increase the number of colonies and coloured antigen yield of Mycoplasma gallisepticum Soeripto .
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 3 (1997)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A trial to increase the number of colonies and coloured antigen yield of Mycoplasma gallisepticum in mycoplasma broth medium was conducted by comparing the use of normal medium, medium with sodium pyruvate and medium with sodium hydroxide. The result showed that medium with §odium pyruvate had increased the number of colonies and antigen yield and was highly significant different (P< 0.01) compared to both normal medium and medium with sodium hydroxide. Medium with sodium hydroxide produced the number of colonies and antigen yield lower than medium with sodium pyruvate, but higher and was highly significant different (P< 0.01) compared to the normalmedium. Key words: Sodium pyruvate, sodium hydroxide, Mycoplasma gallisepticum antigen

Sensitivity of some local isolates of Mycoplasma gallisepticum against antibiotics Sutiastuti Wahyuwardani; Soeripto .
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 1 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Sensitivity of five local isolates ofMycoplasma gallisepticum (MG) strain and two standard MG isolates obtained from Australia were tested against antibiotics of oxytetracycline, doxycycline, erythromycin, bacitracin, vancomycin, methicillin and penicillin using antibiogram disc method. The result showed that one, 2 and 3 local MG isolates were resistent to doxycycline, erythromycin and oxytetracycline respectively . MG isolate of ADA7 from Australia was found to be resistent to all antibiotics tested. None ofthe local MG isolates were sensitive against bacitracin, vancomycin, methicillin and penicillin. Key words: Mycoplasma gallisepticum, antibiogram disc

Chronic Respiratory Disease (Crd) of Chicken Soeripto .
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 19, No 3 (2009): SEPTEMBER 2009
Publisher : Indonesian Center for Animal Research and Development

Chronic respiratory disease (CRD) of chicken is the most costly disease confronting poultry industries in the world. The economic losses due to CRD was estimated up to billions rupiahs per year in Indonesia, and in the USA was estimated up to hundred millions dollars per year. The losses mainly due to decreases of body weight gain, egg production, feed efficiencies, hatchabilities and increases of embryo mortality. The main causative agent of CRD is Mycoplasma gallisepticum (MG). Respiratory disturbances, excretion of nasal exudate, coughing, sneezing and hyperaemic of the conjunctiva are very often seen as the clinical signs. Pathological lesions are often found as inflammation of respiratory organs and more specific lesions are seen as inflammation and thickening of the airsac membranes with foci cheesy materials scattered around the airsacs. Diagnosis of CRD can be made by clinical symptoms, serology examination dan isolation of MG. Treatment, prevention and controls of CRD have been carried out for years, but cases of CRD are still present up to now. The MGTS11 vaccine as the third generation of CRD vaccine was reported to be effective for controlling CRD of chickens and potentially used as a tool for eradication programme of CRD in the future. Key words: Chronic respiratory disease, chicken

Uji Lapang Vaksin Mycoli Untuk Pencegahan CRD Pada Ayam Potong = Field Trial of Mycoli Vaccine for CRD Protection in Broiler Chickens. Soeripto .; Andriani .
Jurnal Sain Veteriner Vol 24, No 1 (2006): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Field trial of Mycoli vaccine was carried out on a commercial broiler farm in West Java, Indonesia. The number of vaccinated chickens was 3.000 heads and another 3.000 heads were used as control. The route of vaccination was done via subcutaneous tissue behind the head with a dose of 0.2 mIthead. The vaccination was given at 4 days of age concurrently with ND vaccine adminitration. As a field control, a number of 50 vaccinated chickens designated as Group I and another 50 non vaccinated chickens designated as Group II taken from the field trial were kept in Balitvet pens. Each of the Group was then divided again into 10 subgroups of 5 heads, kept in wire cages. At 4 weeks of age, chickens in 5 subgroups of Group I and of Group II were challenged with wild strain of Mycoplasma gallisepticum (MG) R980 via abdominal airsacs. Blood samples were collected from all chickens before challenged and before termination day for serology MG antibody. Feed and body weight gain were measured every week. All simulated chickens at Balitvet were killed at 42 days of age and examined for pathological lesions. The results of field trial showed that vaccinated chickens had produced body weight gain of 510g and feed conversion of 0.04 per head better than non-vaccinated groups, but statistically no difference between vaccinated and control chickens. The simulated chickens at Balitvet showed that chickens of Group I had shown better protection against MG challenge than chickens of Group H. The vaccinated chickens produced body weight gain of 39g and rate of feed conversion of 0.35 per head better than control chickens, and the vaccinated challenged chickens had body weight gain and feed conversion of 48g and 0.34, respectively better than the control challenged chickens.

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