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All Journal Jurnal Veteriner Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Jurnal Kedokteran Hewan

Sutiastuti Wahyuwardani

Unknown Affiliation

Author-ID : 2823475

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Articles

The influence of body development of adult female Boophilus microplus ticks on their eggs fertility Sutiastuti Wahyuwardani
Jurnal Ilmu Ternak dan Veteriner Vol 1, No 1 (1995)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

One hundred and seventy six adult female cattle ticks, Boophilus microplus, were collected to observed the influence of their body development on their eggs fertility under laboratory conditions (22°-32°C and 84-92% relative humidity) . The regression analysis result shown that as the body developed, eggs production and eggs fertility increased, but there was no correlation between ticks weight and the incubation periode . The relation between ticks weight and eggs production was shown as Y = -309 .433 + 13 .868X and r = 98 .5 % whereas the influence of ticks body development on eggs fertility was shown as Y = 8 .224 + 0 .338X, r = 92 .0% and the incubation periode was about 21-26 days . The fertile eggs begin to be produced by ticks with body weight of about 35-49 mg and fertility 28.39%.

The macroscopic and microscopic patology changes on broiler infected with local reovirus isolate Sutiastuti Wahyuwardani; Hernomoadi Huminto; Lies Parede
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 1 (2005): MARCH 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Local reovirus isolate is the virus which could be isolated from runting and stunting syndrome. The ability of local reovirus isolate to induce runting and stunting syndrome in broilers chicken was investigated. The day old chicks (doc) were infected with local reovirus isolate assessed clinically and pathologically at 1, 2 and 3 weeks post inoculation. A total of 40 DOC were divided into two groups. The first group (20 doc) was orally infected with ± 2 x 103 local reovirus isolate particle and the other used as negative control group. The results showed that the isolate caused wet droppings, stunting, enteritis, pancreatitis, malabsorbtion, bursal atrophy and spleenic hypertrophy, which similar to runting and stunting syndrome (RSS) disease in chicken. The body weight was reduced to 14.7% on the inoculated group at 4 weeks post inoculation. Key Words: Broiler, Local Reovirus Isolate, Runting and Stunting, Malabsorption

Sensitivity of some local isolates of Mycoplasma gallisepticum against antibiotics Sutiastuti Wahyuwardani; Soeripto .
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 1 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Sensitivity of five local isolates ofMycoplasma gallisepticum (MG) strain and two standard MG isolates obtained from Australia were tested against antibiotics of oxytetracycline, doxycycline, erythromycin, bacitracin, vancomycin, methicillin and penicillin using antibiogram disc method. The result showed that one, 2 and 3 local MG isolates were resistent to doxycycline, erythromycin and oxytetracycline respectively . MG isolate of ADA7 from Australia was found to be resistent to all antibiotics tested. None ofthe local MG isolates were sensitive against bacitracin, vancomycin, methicillin and penicillin. Key words: Mycoplasma gallisepticum, antibiogram disc

Infection of Chicken Anaemia Virus: Etiology, Epidemiology, Clinical Sign, Pathological Changes and Disease Control Sutiastuti Wahyuwardani; Tatty Syafriati
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 15, No 3 (2005): SEPTEMBER 2005
Publisher : Indonesian Center for Animal Research and Development

Initially, Chicken anaemia virus (CAV) is known as CAA, which was first isolated by Yuasa in Japan in 1976. CAV particles are non enveloped with a diameter of 19 .1-20 .7nm, belonging to the family Circoviridae, genus Gyrovirus . CAV infection was first appeared in Indonesia at the same time as the outbreak of stunting and runting syndrome in 1996 with a mortality rate of 5-15% but it may reach to 60%. CAV can be transmitted vertically or horizontally. Chicken all ages is susceptible to infection. Infection of CAV occurred in young chicken flock at 2-3 weeks growth of age, causing clinical signs while in old chicken flock which is sub clinical. The signs of infectious of CAV are retarded, anaemia, anorexia, pale of face and pial. The pathology anatomy changes are pale carcases, yellowish bone marrow, atrophy of thymus and bursa fabricius . Whereas, histophatological changes are thymic necrosis of cortex and medulla, lymphocyte depletion of thymus, bursa fabricius and bone marrow. Diagnose of CAV is based on pathological changes and followed by the isolation of certain lymphoblastoid chicken cell MDCC-MSB I and then, is identified by virus neutralization. The presence of virus can also be identified by immunofluorescent and immunoperoxidase staining, in situ hybridization technique or PCR. For antibody detection, ELISA technique can be used. The syndromes of CAV infection are closely associated with those of osteopetrosis, reovirus, infectious bursal disease (IBD) and Marek. Vaccination programme in breeding farm is needed to induce maternal antibody. This paper describes the CAV disease and its occurrence in Indonesia. Key word : Chicken anaemia virus, diagnose, pathology anatomy, histopathology, control

Patogenesitas Virus Gumboro Isolat Lokal pada Ayam Pedaging Sutiastuti Wahyuwardani; Dewi Ratih Agungpriyono; Lies Parede; Wasmen Manalu
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Study of the pathogenicity of gumboro virus (very virulent Infectious Bursal Disease virus, vvIBDV) oflocal isolates was performed in broilers. The chickens were grouped into four: i) infected with vvIBDV; ii)vaccinated with commercial vaccine + challenge with vvIBDV; iii) vaccinated using locally produced vaccine+ challenged with vvIBDV; and iv) control group (unvaccinated animals). Pathogenecity was analyzedbased on the sequence of infection; the distribution and degree of gross pathology and histopathologylesions on bursa Fabricius, spleen and thymus in relation to the presence of the antigen (IBDV Ag) whichwas detected using immunohistochemistry technique. During the acute phase (1-3 days following theexperimental infection) hyperemia and exudation on the bursa Fabricius were observed in the infectedgroup and the two vaccinated-challenged groups. Similarly, hyperemia was also observed on the spleenand thymus. Whilst during the chronic stages (at 7 and 14 days following the experimental infection) thebursa showed atrophy. Upon histological examination, the bursa showed interstitial edema, hemorrhage,infiltration of heterophyl cells and fibroblast hyperplasia. The bursa lymphoid follicles were depleted dueto cells necrosis and apoptosis. Plica epithelial layer was wrinkled and the Goblet cells metaplasia formedhyper plastic cysts. In the spleen and thymus severe reticulo endothelial cells proliferation was observedduring the acute stage and tended to decline during the chronic stage. During the acute and chronic stagescells containing IBDV Ag were detected in the bursa Fabricius, distributed within the mantle zone andgerminal center of lymphoid follicles in the two vaccinated-challenge groups. Whereas in the infectedgroup, the IBDV antigen were detected up to 7 days post the infection. Nevertheless, neither IBDV antigenwas detected in the spleen nor in the thymus in all the experimental groups. The results of this studyclearly explained the ferocity of vvIBDV of local isolates.

STUDI KASUS PENYAKIT AYAM PEDAGING DI KABUPATEN SUKABUMI DAN BOGOR Ening Wiedosari; Sutiastuti Wahyuwardani
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui kejadian penyakit secara patologis pada ayam pedaging di Kabupaten Sukabumi dan Bogor pada bulan Februari (musim penghujan) dan Juni (musim kemarau) tahun 2012. Dari sejumlah peternakan yang dikunjungi total diperoleh 40 kasus penyakit. Diagnosis penyakit ditetapkan berdasarkan sejarah penyakit yang terjadi di peternakan, umur ayam yang sakit, gejala klinis, perubahan patologi anatomis, dan histopatologis. Berbagai faktor seperti kondisi iklim, tata laksana peternakan, pengobatan, dan sejarah vaksinasi digunakan sebagai data penunjang. Hasil penelitian menunjukkan bahwa penyakit yang teridentifikasi adalah colibacillosis (22,2%), asites (12,5%), gumboro (12,5%), Newcastle disease (ND) (10%), Salmonella pullorum (10%), dan necrotic enteritis (7,5%). Penyakit terutama terjadi pada ayam umur 11-21 hari (57,5%) dan terjadi pada musim penghujan (60%).

Co-Authors Dewi Ratih Agungpriyono Ening Wiedosari Hernomoadi Huminto Lies Parede Soeripto . Tatty Syafriati Wasmen Manalu

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