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All Journal Jurnal Sain Peternakan Indonesia Journal of the Indonesian Tropical Animal Agriculture
Harahap, R. S.
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Veterinary

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Association of Genetic Polymorphism TP53INP1 Gene with Mineral Content in Javanese Thin-Tailed Sheep Listyarini, K.; Harahap, R. S.; Roosita, K.; Sumantri, C.; Mulyono, R. H.; Gunawan, A.
Jurnal Sain Peternakan Indonesia Vol 20 No 2 (2025)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.20.2.88-92

Abstract

The Tumor Protein P53 Inducible Nuclear Protein 1 (TP53INP1) gene is expected to be a crucial candidate for mineral content.  This research aimed to analyse the genotype polymorphism and investigate its association with the TP53INP1 gene and sheep mineral content. To analyse gene polymorphisms and conduct an association study, 30 rams of Javanese thin-tailed sheep were utilised. The NlaIII restriction enzyme was applied to investigate the genotype polymorphism of the TP53INP1 gene through Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Regarding the association with analysing TP53INP1, a General Linear Model (GLM) was used. The findings indicate that the TP53INP1 gene shows polymorphism, revealing three distinct genotypes identified by the results: AA, AG, and GG. The genotypes were detected using the Hardy-Weinberg Equilibrium (HWE) principle. According to the association analysis, a significant association (P < 0.05) was found between the TP53INP1 gene and mineral content, particularly iron (Fe). A higher mineral content was associated with the GG genotype, whereas a lower mineral content was related to the AA genotype. The TP53INP1 could be the candidate gene for sheep's mineral content.
Polymorphism of BGN gene (g.77807325 G>C) and its association with fatty acid and carcass characteristics of Indonesian meat lamb Amin, M. F.; Sumantri, C.; Arief, I. I.; Jayanegara, A.; Listyarini, K.; Harahap, R. S.; Gunawan, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 2 (2024): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.2.125-134

Abstract

Fatty acid content and carcass characteristics are two meat quality traits consumers choose to ob tain healthy and high-quality lamb. Genetic improvement of fatty acid content and carcass characteris tics in sheep is important to increase the public appeal of lamb meat. The Biglycan gene (BGN) is a gene that functions to regulate the growth and maintenance of connective tissues, such as bone and cartilage. This study examined the polymorphism of the BGN gene to get a deeper understanding of the correlation between fatty acid content and carcass traits in Indonesian sheep. The PCR-RFLP ap proach was used to detect polymorphism in the BGN gene in meat samples obtained from the longissi mus dorsi region of 115 rams aged between 10 and 12 months. The General Linear Model (GLM) was used test to analyze genotyping through association studies between genotypes and phenotypic attrib utes. The results indicated that the carcass' fatty acid composition, including tridecanoic acid (C13:0), arachidonic acid (C20:4n-6), and polyunsaturated fatty acids (PUFA), as well as the BGN gene with SNP point g. 77807325 G>C (GG and CC genotypes), were significantly (P<0.05) related to these traits. Genotype GG was more associated with carcass percentage traits than other genotypes, whereas genotype CC was best associated with carcass length traits. The BGN gene with SNP point g. 77807325 G>C can be a potential genetic marker for selecting fatty acid (tridecanoic acid C13:0), pol yunsaturated fatty acid (PUFA), arachidonic acid), carcass percentage, and carcass length in sheep meat in Indonesia.
Co-Authors A. GUNAWAN Amin, M. F. Anuraga Jayanegara Arief, I. I. C. SUMANTRI Listyarini, K. Mulyono, R. H. Roosita, K.