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PENGARUH PENAMBAHAN PREBIOTIK INULIN TERHADAP AKTIVITAS ANTIBAKTERI LACTOBACILLUS CASEI Siregar, Hafiz Hawari; Silaban, Agnes Novica Putri; Sofiyana, Maria; Purba, Rosi Krisdayanti; Intisari, Yayo; Lister, I Nyoman Ehrich; Fachrial, Edy
Preventif Journal Vol 6, No 1 (2021): Preventif Journal
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37887/epj.v6i1.22594

Abstract

AbstrakInulin adalah salah satu prebiotik yang banyak dipergunakan sebagai sumber nutrisi bagi probiotik. Tujuan penelitian ini ialah untuk mengetahui efek penambahan prebiotik inulin pada aktivitas antibakteri Lactobacillus casei. Metode antibakteri dilakukan dengan metode difusi cakram. Dalam percobaan terpisah bakteri asam laktat yang telah diuji kemudian diinokulasi ke dalam tabung reaksi yang berisi MRS Broth 10 mL dan MRS Broth 10 mL yang telah ditambahkan dengan 5% tepung inulin. Kemudian diinkubasi dengan suhu 37 °C selama 24 jam.  Kemudian dilakukan kultur Bakteri patogen (Staphylococcus aureus) pada Nutrien Agar dengan cara mencelup kapas steril ke dalam tabung reaksi yang berisi bakteri patogen dan kemudian menyebarkannya secara merata pada Nutrien Agar steril dalam cawan petri. Nutrien Agar dalam cawan petri yang sudah diinokulasi pada suhu 37 °C selama 24 jam dengan bakteri patogen diinkubasikan. Hasil isolasi menunjukkan bahwa probiotik L. casei yang diisolasi dari minuman susu komersil memiliki aktivitas antibakteri spektrum luas. Probiotik L. casei ini efektif menghambat pertumbuhan bakteri S. aureus. Penambahan konsentrasi prebiotik inulin 0,5% (b/v) pada MRSB dapat menambah daya hambat bakteri Lactobacillus casei sebesar 8,4 mm terhadap pertumbuhan bakteri S.aureus. Untuk penelitan selanjutnya, perlu dilakukan pengamatan tentang berat molekul dan golongan bakteriosin yang dihasilkan oleh L. casei. Kata Kunci : Prebiotik, Inulin, Antibakteri, Lactobacillus casei
Evaluasi hambatan waktu penyediaan dokumen rekam medis elektronik di RS Murni Teguh Medan Nasution, Sri Lestari Ramadhani; Siregar, Hafiz Hawari; Adnan, Maco Chan; Girsang, Ermi
Jurnal Penelitian Pendidikan IPA Vol 9 No 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.4670

Abstract

The impact of errors in storing medical records causes delays for officers in searching for medical record documents so that services at the polyclinic are hampered. It is necessary to control the incidence of incorrect insertion of medical record documents so that the incidence of incorrect insertion of medical record documents can be reduced. Electronic Medical Record (RME) is a form of health information service that is computerized and recorded. Health service facilities that implement RME in an effort to improve the quality and quality of services. This research is a qualitative research, case study design which aims to evaluate the timing of providing electronic medical record documents for outpatients at Murni Teguh Hospital. Determination of research subjects using purposive sampling technique. The research subjects consisted of the Director of Murni Teguh Hospital, the head of the Medical Record Installation as the main informant, the Patient Registration Officer, and 3 medical record staff as triangulation informants. In collecting data, researchers conducted in-depth interviews, direct observation and document studies. Data analysis was carried out using interactive models, data reduction processes, verification and drawing conclusions. In this study, it was found that the time required to provide electronic medical record documents for outpatients at Murni Teguh Hospital was a minimum of 4 minutes, a maximum time of 9 minutes with an average time needed to provide electronic medical record documents was 6.6 minutes. Obstacles encountered are errors during use, as well as HR who still do not understand the use of electronic medical record systems. By providing electronic medical record documents, the work of the patient medical record department becomes more efficient, makes it easier for officers to search for patient medical records and the benefits that patients get are shorter waiting times.