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Molecular Identification and Diversity of Yeasts Associated with Apis cerana Foraging on Flowers of Jatropha integerrima ADI BASUKRIADI; WELLYZAR SJAMSURIDZAL; BANGGA BERISTAMA PUTRA
Microbiology Indonesia Vol. 4 No. 1 (2010): April 2010
Publisher : Indonesian Society for microbiology

There are only a few reports from tropical countries, and none from Indonesia, on yeasts associated with the Asiatic honeybee, Apis cerana. Here we report on yeasts associated with A. cerana foraging on flowers of Jatropha integerrima in the campus of the Universitas Indonesia, Depok, Indonesia. Yeasts were isolated from guts of 30 individual pollen-collecting bees (PCB) and nectar-collecting bees (NCB), and identified by their internal transcribed spacer (ITS) regions of their rDNA sequences. Based on ITS regions sequence data, 14 representative yeast isolates obtained from A. cerana were found to be closely related to Aureobasidium pullulans, Dothioraceae sp., Candida cf. apicola, C. cf. azyma, C. cellae, Metschnikowia sp., Kodamaea ohmeri and Yarrowia lipolytica. Undescribed yeast of the genus of Metschnikowia was also discovered in this study. At present, we assume there is association between C. cf. apicola and species closely related to C. cellae with A. cerana. Yeasts species associated with PCB differ from those found in NCB, indicating that PCB and NCB possess different and specific yeasts communities. Some yeasts species isolated from A. cerana show a low degree of similarity to their closest related species. Our study sheds light on the detection of several new taxa of yeasts associated with A. cerana.

Insecticidal Activities of Ethyl Acetate Extract of Indonesian Mangrove Fungus Emericella nidulans BPPTCC 6038 on Spodoptera litura SILVA ABRAHAM; ADI BASUKRIADI; SUYANTO PAWIROHARSONO; WELLYZAR SJAMSURIDZAL
Microbiology Indonesia Vol. 9 No. 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

Mangrove fungi are known as sources of biological active compounds. The study and the report of secondary metabolites of mangrove fungi as insecticides is very limited in Indonesia. This study assess the insecticidal activities of ethyl acetate extract of Indonesian mangrove fungus Emericella nidulans BPPTCC 6038 against Spodoptera litura (Lepidoptera, Noctuidae) neonate larvae and pupae. The fungus E. nidulans BPPTCC 6038 was isolated from leaves of mangrove Rhizophora mucronata and identified based on ITS rDNA sequence data, with the GenBank accession number KP165435, and confirmed with morphological observation. This fungus strain was grown on malt extract broth for 14 days on rotary shaker at 65 rpm, and incubated at room temperature. Mortalities of S. litura were observed on larvae fed on artificial diet containing ethyl acetate extract of E. nidulans at concentrations of 625 â€“ 5000 ppm. The lethal concentration of the extract which causes 50% mortality of larvae (LC50 value) was 1102.27 ppm. The other effects of fungus extract on S. litura were decrease in growth rate, longer larval period, inhibition on pupal development and absence in adult emergence. The HPLC analysis of extract showed that the crude extract contained three major compounds. This study provides evidence that the extract of E. nidulans possesses insecticidal activities against S. litura.

MODELLING THE POTENTIAL DISTRIBUTIONS OF SAWO KECIK (MANILKARA KAUKI (L.)) DUBARD USING MAXENT TO SUPPORT CONSERVATIONS OF HISTORICAL AND CULTURAL VEGETATIONS IN DAERAH ISTIMEWA YOGYAKARTA PROVINCE Andri Wibowo; Atus Syahbudin; Adi Basukriadi; Erwin Nurdin
Reinwardtia Vol. 22 No. 1 (2023)
Publisher : BRIN Publishing (Penerbit BRIN)

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Modelling the potential distributions of Sawo Kecik (Manilkara kauki (L.)) Dubard using MaxEnt to support conservations of historical and cultural vegeta-tions in Daerah Istimewa Yogyakarta Province. Reinwardtia 22(1): 55‒67. — Sawo kecik or Manilkara kauki (L.) Dubard, of the Sapotaceae family as it is formally known, is one of the species with significant cultural values in Yog-yakarta Province (DIY) culture because it symbolizes social righteousness. In connection with this, Yogyakarta's mu-nicipal and district governments have been encouraged to plant sawo kecik. Despite these efforts, there is still a lack of knowledge regarding the possible range of this species, and this knowledge is essential to promoting the conservation of M. kauki in DIY. With the help of isothermality, precipitation of driest month, precipitation seasonality, precipita-tion of driest quarter, and precipitation of warmest quarter data, this study tries to simulate the probable distributions of M. kauki throughout cities and districts in DIY. The model estimated 1,275 km2 of DIY areas was suitable for M. kauki that concentrated in the central parts, spanning from the west to the east of DIY. Yogyakarta City followed by Sleman District has the largest areas categorized from high to very high suitable for M. kauki. While, Gunung Kidul followed by Kulonprogo Districts have the largest areas categorized as low suitable. To conclude, M. kauki can adapt areas with moderate precipitation as low as 20 mm during driest month and as low as 100 mm during driest quarter. During warmest quarter, M. kauki requires precipitation with value of 700 mm. The conservation effort and M. kauki planting should then concentrate on Yogyakarta City and Sleman District since such locations are thought to have high appropriateness for the species.

MODELLING THE POTENTIAL DISTRIBUTIONS OF SAWO KECIK (MANILKARA KAUKI (L.)) DUBARD USING MAXENT TO SUPPORT CONSERVATIONS OF HISTORICAL AND CULTURAL VEGETATIONS IN DAERAH ISTIMEWA YOGYAKARTA PROVINCE Andri Wibowo; Atus Syahbudin; Adi Basukriadi; Erwin Nurdin
Reinwardtia Vol. 22 No. 1 (2023)
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Modelling the potential distributions of Sawo Kecik (Manilkara kauki (L.)) Dubard using MaxEnt to support conservations of historical and cultural vegeta-tions in Daerah Istimewa Yogyakarta Province. Reinwardtia 22(1): 55‒67. — Sawo kecik or Manilkara kauki (L.) Dubard, of the Sapotaceae family as it is formally known, is one of the species with significant cultural values in Yog-yakarta Province (DIY) culture because it symbolizes social righteousness. In connection with this, Yogyakarta's mu-nicipal and district governments have been encouraged to plant sawo kecik. Despite these efforts, there is still a lack of knowledge regarding the possible range of this species, and this knowledge is essential to promoting the conservation of M. kauki in DIY. With the help of isothermality, precipitation of driest month, precipitation seasonality, precipita-tion of driest quarter, and precipitation of warmest quarter data, this study tries to simulate the probable distributions of M. kauki throughout cities and districts in DIY. The model estimated 1,275 km2 of DIY areas was suitable for M. kauki that concentrated in the central parts, spanning from the west to the east of DIY. Yogyakarta City followed by Sleman District has the largest areas categorized from high to very high suitable for M. kauki. While, Gunung Kidul followed by Kulonprogo Districts have the largest areas categorized as low suitable. To conclude, M. kauki can adapt areas with moderate precipitation as low as 20 mm during driest month and as low as 100 mm during driest quarter. During warmest quarter, M. kauki requires precipitation with value of 700 mm. The conservation effort and M. kauki planting should then concentrate on Yogyakarta City and Sleman District since such locations are thought to have high appropriateness for the species.

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